Search results for "ATP"

Autovadītāju psiholoģiskā skatpunkta skalas veidošana un sākotnējā validitātes pārbaude

2022

Pētījuma mērķis bija izveidot Autovadītāju psiholoģiskā skatpunkta skalu un veikt tās pirmreizējās validitātes pārbaudes. Pētījuma rezultātā izveidota Autovadītāju psiholoģiskā skatpunkta skala ar četrām apakšskalām, kas raksturo autovadītāju psiholoģiskā skatpunkta dimensijas – piesardzību, sadarbību, telpu un spriešanu. Pētījumā identificēti turpmākie skalas pilnveidošanas un izstrādes virzieni. Pētījumā piedalījās 648 autovadītāji. Autovadītāju psiholoģiskā skatpunkta skalas validitātes pārbaudēs izmantota Autovadītāju uzvedības aptauja (Reason et al., 1990), Starppersonu reakciju aptaujas (Davis, 1980) Psiholoģiskā skatpunkta skala un Empātisko rūpju skala, kā arī Pozitīvas autovadītāju…

The inhibition of Ca2+-ATPases of human erythrocyte membranes by covalent binding of ATP derivatives.

1982

The Low-Affinity ATP Binding Site of the Escherichia coli SecA Dimer Is Localized at the Subunit Interface

1997

The homodimeric SecA protein is the ATP-dependent force generator in the Escherichia coli precursor protein translocation cascade. SecA contains two essential nucleotide binding sites (NBSs), i.e., NBS1 and NBS2 that hind ATP with high and low affinity, respectively. The photoactivatable bifunctional cross-linking agent 3'-arylazido-8-azidoadenosine 5'-triphosphate (diN(3)ATP) was used to investigate the spatial arrangement of the nucleotide binding sites of SecA, DiN(3)ATP is an authentic ATP analogue as it supports SecA-dependent precursor protein translocation and translocation ATPase, UV-induced photo-cross-linking of the diN(3)ATP-bound SecA results in the formation of stable dimeric s…

Identification of Novel Anthracycline Resistance Genes and Their Inhibitors

2021

Differentially expressed genes have been previously identified by us in multidrug-resistant tumor cells mainly resistant to doxorubicin. In the present study, we exemplarily focused on some of these genes to investigate their causative relationship with drug resistance. HMOX1, NEIL2, and PRKCA were overexpressed by lentiviral-plasmid-based transfection of HEK293 cells. An in silico drug repurposing approach was applied using virtual screening and molecular docking of FDA-approved drugs to identify inhibitors of these new drug-resistant genes. Overexpression of the selected genes conferred resistance to doxorubicin and daunorubicin but not to vincristine, docetaxel, and cisplatin, indicating…

Latviešu valodas apguves sekmēšana pirmsskolas vecuma bērniem bilingvālā vidē mazākumtautību pirmsskolas izglītības iestādē

2021

Bakalaura darba tēma “Latviešu valodas apguves sekmēšana pirmsskolas vecuma bērniem bilingvālā vidē mazākumtautību pirmsskolas izglītības iestādē” Darba autore: Arita Tirne Pētījuma mērķis: teorētiski pētīt divvalodīgu bērnu valodas apguves sekmēšanu pirmsskolas vecumā un apkopot ieteikumus. Darbs sastāv no divām nodaļām – teorētiskās un empīriskās. Teorētiskajā nodaļā tiek analizēta zinatniskā literatūra. Kā arī izpētīts bilingvālās izglītības mērķis bērnu attīstībā un bilingvālas izglītības īpatnības pirmsskolas izglītības līmenī. Tiek pamatots, cik bilingvālā izglītība ir nozīmīga runas un intelekta attīstībai. Kā arī atspoguļota bilingvālā attīstība pirmsskolas vecuma bērniem, faktori, …

Is ATP Hydrolysis the Power Stroke in ABC Transporters?

2018

Lack of Cry1Fa binding to the midgut brush border membrane in a resistant colony of Plutella xylostella moths with a mutaton in the ABCC2 locus

2012

ABSTRACT Previous studies reported “mode 1” Bacillus thuringiensis resistance in a colony of diamondback moths (NO-QA), and recently, this resistance has been mapped to an ABC transporter ( ABCC2 ) locus. We report the lack of binding of Cry1Fa to insects derived from this colony and compare our data with those from other insects with ABCC2 -associated resistance.

Insulin resistance and endogenous digoxin-like factor in obese hypertensive patients with glucose intolerance

1992

Hypertensive obese subjects with glucose intolerance have hyperinsulinaemia, insulin resistance and intracellular cation imbalance resulting in increased sodium content. The aim of our study was to assess in these patients plasma levels of endogenous digoxin-like factor (EDLF), an inhibitor of the sodium-pump mechanism. We studied 14 hypertensive and 12 normotensive subjects with obesity and glucose intolerance for fasting blood glucose, and plasma insulin, C-peptide and EDLF levels: the two groups were matched for age and BMI and were studied after a 2-week wash-out period from hypotensive drugs. Compared with normotensives, hypertensive subjects had higher plasma insulin levels, a greater…

Experimental diabetic neuropathy: role of oxidative stress and mechanisms involved.

1998

Oxidative stress has been related to the development of diabetic neuropathy. Experimental diabetes (alloxan injection to mice) promotes early biochemical changes in peripheral nervous tissue, e.g., decrease in Na,K-ATPase activity and glutathione (GSH) peroxidase (GSHPx) activity. The former decrease can be reverted by inhibiting protein kinase C (PKC), since it has been reported that PKC is activated in these experimental conditions. Here we present data demonstrating that the inhibition of PKC, as early as 4 days after alloxan administration, is not able to return to normal values GSHPx activity in sciatic nerve of diabetic mice. Thus, it would fit with our previous proposal of the possib…

Decreased glutathione peroxidase activity in sciatic nerve of alloxan-induced diabetic mice and its correlation with blood glucose levels.

1993

The effect of alloxan-induced diabetes on glutathione peroxidase (GSH-Px) activity in sciatic nerve of mice has been studied. We have found, 7 days after alloxan treatment, a significant decrease in this enzymatic activity in the cytosol of sciatic nerve of diabetic mice, and moreover, that these changes remained unaltered up to 21 days after alloxan injection. No modification in the glutathione content of sciatic nerve of diabetic mice was observed throughout the experiment when compared with controls. The decrease in GSH-Px activity in this tissue shows a good correlation with the increase of blood glucose levels throughout the experiment. It is hypothesized whether a combination of mecha…