Search results for "AUV"
showing 10 items of 724 documents
La mauvaise foi entre discours, éthique et éristique
2022
In the field of critical discourse analysis "bad faith" is hardly a concept and yet stands as an intuitive dimension of speech conventions. It spans across rhetorics, ethics, semantics, reasoning and argumentation. This article and the overall volume which it introduces is interested in trying to develop formal criteria to distinguish bad faith from denial, lying, or manipulation. The aim is to provide linguistic evidence of its inner workings in relation to pragmatical and psychological factors. As it appears in eristic contexts, it takes part in the global agonistic approach to interactions, in particular in arguments about politics, in media coverage and epistemological debates. Our clai…
L'azote pour contrôler la croissance des mauvaises herbes
2018
Utilizing genetically engineered bacteria to produce plant-specific glucosides
2001
Plant-derived glucosides have attracted much attention due to their widespread applications. This class of products is difficult to isolate or to synthesize in pure form because of the resulting low yields. Thus, simple approaches for the generation of such glucosides would be highly beneficial. We purified and characterized a novel glucosyltransferase from plant cell suspension cultures of Rauvolfia serpentina, which showed rather low substrate specificity. We obtained its cDNA and expressed the active recombinant protein in bacteria (Escherichia coli) with excellent plant-specific glucosylation efficiencies. Compared with the plant system, the bacteria delivered the new enzyme, which was …
Conclusions and Suggestions for Improving European Education Policies
2020
In the concluding chapter, we draw together the main arguments and results of the research, highlighting the differences and similarities between the education policy documents produced by the European Union and the Council of Europe. We also provide suggestions for improving future education policies to better encompass the concerns about the lack of dialogue in a diversified but also polarized Europe. Education policy documents dealing with intercultural dialogue act as important guidelines for tackling racism, chauvinism, xenophobia, homophobia, and other forms of prejudice in Europe. As policies function as ‘actants’ that create webs of meanings and action, it is crucial that policy doc…
High speed X-ray analysis of plant enzymes at room temperature.
2013
X-ray measurements at room temperature (295 K) deliver high quality data sets with unprecedented speed (2 min), as shown for crystallized raucaffricine-O-β-D-glucosidase (RG), its mutant RG-Glu186Gln and several ligand complexes of the enzyme which participates in alkaloid biosynthesis in the plant Rauvolfia. The data obtained are compared with data sets measured under typical cryo conditions (100K). Under both conditions, density maps are highly comparable and favor the described protocol for room temperature measurements, potentially paving the way for future crystallographic studies capturing biosynthetic pathway intermediates.
Natural products and enzymes from plant cell cultures
1995
Plants represent an unlimited source of natural products. Many of the recently detected phytochemicals exhibit remarkable bioactivities, ranging from anticancer activity, phosphodiesterase inhibition to cytotoxicity against HIV-infected cells. Cultivated plant cells produce at their unorganized, dedifferentiated stage secondary metabolites, but in very different amounts in so far as new compounds are concerned. In fact, more than 140 novel natural products are presently known from plant cell cultures, which also include new metabolites formed by biotransformation. The biotransformation capacity of suspended cells is described and recent high yielding transformations, like the formation of a…
The gene encoding polyneuridine aldehyde esterase of monoterpenoid indole alkaloid biosynthesis in plants is an ortholog of theα/β hydrolase super fa…
2000
The biosynthesis of the anti-arrhythmic alkaloid ajmaline is catalysed by more than 10 specific enzymes. In this multistep process polyneuridine aldehyde esterase (PNAE) catalyses a central reaction by transforming polyneuridine aldehyde into epi-vellosimine, which is the immediate precursor for the synthesis of the ajmalane skeleton. PNAE was purified from cell suspension cultures of Rauvolfia serpentina. The N-terminal sequence and endoproteinase LysC fragments of the purified protein were used for primer design and for the amplification of specific PCR products leading to the isolation of PNAE-encoding cDNA from a R. serpentina library. The PNAE cDNA was fused with a C-terminal His-tag, …
Oxidative damages in cho-k1 cells treated with beauvericin
2013
Risk assessment of beauvericin, enniatins and fusaproliferin present in follow-up infant formula by in vitro evaluation of the duodenal and colonic b…
2014
Abstract In this study, 72 samples of follow-up infant formula of Spanish origin were analyzed for the presence of the mycotoxins beauvericin (BEA), enniatins (ENs) (A, A1, B, B1), and fusaproliferin (FUS). The samples analyzed were extracted three times with ethyl acetate and then the mycotoxins were identified and quantified using a liquid chromatography (LC) coupled to a diode array detector (DAD). The positive samples analyzed in this study were digested through a simulated gastrointestinal digestion model, which permit to simulate the physiological condition of the human gastrointestinal tract (duodenal and colonic compartments) in order to assess the bioaccessibility of the bioactive …