Search results for "Acetoacetates"
showing 3 items of 3 documents
Metabolomics of Human Amniotic Fluid and Maternal Plasma during Normal Pregnancy
2016
Metabolic profiles of amniotic fluid and maternal blood are sources of valuable information about fetus development and can be potentially useful in diagnosis of pregnancy disorders. In this study, we applied 1H NMR-based metabolic profiling to track metabolic changes occurring in amniotic fluid (AF) and plasma (PL) of healthy mothers over the course of pregnancy. AF and PL samples were collected in the 2nd (T2) and 3rd (T3) trimester, prolonged pregnancy (PP) until time of delivery (TD). A multivariate data analysis of both biofluids reviled a metabolic switch-like transition between 2nd and 3rd trimester, which was followed by metabolic stabilization throughout the rest of pregnancy proba…
Dynamics of ketone body metabolism in diabetic rats.
1972
Steady state blood levels of ketone bodies during infusions of acetoacetate at various rates have been compared in healthy and diabetic rats. The characteristics of the metabolic elimination of ketone bodies from the blood are completely changed in diabetic rats. Whereas steady state levels of ketone bodies increase linearly with the infusion rate in healthy rats, this increase is exponential in diabetic animals. This difference, which is due to an impaired metabolic elimination, becomes evident only above a dosage of 50 μmoles acetoacetate per kg per min. Chronic treatment with insulin for 4–6 days, but not acute insulin injection, restores the capacity of diabetic rats to metabolize keton…
Action of metyrapone on the redox state of free nicotinamide-adenine dinucleotide and on oxygen consumption of perfused rat livers and isolated mitoc…
1971
Metyrapone [2-methyl-1,2-bis-(3-pyridyl)-1-propanone] in a concentration of 5 mM increased the lactate/pyruvate ratio and theΒ-hydroxybutyrate/ acetoacetate ratio in the perfusion fluid of the isolated rat liver by a factor of 6 indicating a considerable shift in the ratio of free [NAD]/[NADH] in both the cytoplasmic and the mitochondrial compartment. Oxygen uptake of the isolated liver was decreased to about one half. The onset of the inhibitory effect on the respiration of the isolated organ was immediate. The inhibition lasted for at least 1 h.