6533b823fe1ef96bd127f655
RESEARCH PRODUCT
Action of metyrapone on the redox state of free nicotinamide-adenine dinucleotide and on oxygen consumption of perfused rat livers and isolated mitochondria.
G. F. KahlS. Simonsubject
Malemedicine.medical_specialtychemistry.chemical_elementHydroxybutyratesMitochondria LiverNicotinamide adenine dinucleotideIn Vitro TechniquesOxygenRedoxAcetoacetatesElectron Transportchemistry.chemical_compoundHydroxybutyrate DehydrogenaseOxygen ConsumptionInternal medicineRespirationmedicineAnimalsPyruvatesPharmacologyMetyraponeChemistryGeneral MedicineCompartment (chemistry)MetyraponeNADRatsPerfusionKineticsEndocrinologyBiochemistryLiverCytoplasmSpectrophotometryDepression ChemicalLactatesNAD+ kinasemedicine.drugFerrocyanidesPolarographydescription
Metyrapone [2-methyl-1,2-bis-(3-pyridyl)-1-propanone] in a concentration of 5 mM increased the lactate/pyruvate ratio and theΒ-hydroxybutyrate/ acetoacetate ratio in the perfusion fluid of the isolated rat liver by a factor of 6 indicating a considerable shift in the ratio of free [NAD]/[NADH] in both the cytoplasmic and the mitochondrial compartment. Oxygen uptake of the isolated liver was decreased to about one half. The onset of the inhibitory effect on the respiration of the isolated organ was immediate. The inhibition lasted for at least 1 h.
year | journal | country | edition | language |
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1971-01-01 | Naunyn-Schmiedebergs Archiv fur Pharmakologie |