Search results for "Acinetobacter"
showing 10 items of 44 documents
Biofilmas un metallo-β-laktamāzes producēšanas noteikšana Pseudomonas aeruginosa un Acinetobacter baumannii baktēriju almos
2020
Pseudomonas aeruginosa un Acinetobacter baumannii metallo-β-laktamāžu producēšana ir kļuvusi par pieaugošu terapeitisko problēmu visā pasaulē, kā arī antibiotiku rezistentiem celmiem ir spēja veidot noturīgākas biofilmas, kas rada problēmas šo patogēnu izraisīto infekciju ārstēšanā. Darba mērķis: noteikt Pseudomonas aeruginosa un Acinetobacter baumannii spēju veidot biofilmas, producēt metallo- β-laktamāzes, kā arī statistiski apkopot datus par jutību pret antibakteriālajiem līdzekļiem starp 2017. gadu un 2019. gadu, un veikt rezistences izmaiņas salīdzinājumu. Uzdevumi: identificēt no elpceļiem iegūtos izolātus ar BBL™ Crystal™ sistēmu, kā arī ar GenoType® BC gramnegative testu, veikt anti…
Rapid, cost-effective, sensitive and quantitative detection of Acinetobacter baumannii from pneumonia patients
2011
Background and Objectives: Pneumonia with Acinetobacter baumannii has a major therapeutic problem in health care settings. Decision to initiate correct antibiotic therapy requires rapid identification and quantification of organism. The aim of this study was to develop a rapid and sensitive method for direct detection of A. baumannii from respiratory specimens. Materials and Methods: A Taqman real time PCR based on the sequence of blaoxa-51 was designed and used for direct detection of A. baumannii from 361 respiratory specimens of patients with pneumonia. All specimens were checked by conventional bacteriology in parallel. Results: The new real time PCR could detect less than 200 cfu per m…
Drug resistance patterns of bacteria isolated from patients with nosocomial pneumonia at Tehran hospitals during 2009-2011
2013
Introduction: Nosocomial pneumonia remains an important cause of mortality and morbidity worldwide. Surveillance programs play an important role in the identification of common etiologic agents and local patterns of antimicrobial resistance. Methodology: In this study we determined the frequency and antimicrobial susceptibility of pathogens isolated from patients with nosocomial pneumonia during 2009 to 2011. Results: A total of 642 bacteria were isolated from 516 suspected samples. Acinetobacter baumannii (21.1%, n = 136), was the commonest isolated pathogen followed by Pseudomonas aeruginosa (17.4%, n = 112) , Staphylococcus aureus (15.8%, n = 102) and enterococci (8.4% n = 54). The most …
Cladosins L-O, new hybrid polyketides from the endophytic fungus Cladosporium sphaerospermum WBS017
2019
Abstract The endophytic fungus Cladosporium sphaerospermum WBS017 was obtained from healthy bulbs of Fritillaria unibracteata var. wabuensis. Fermentation of C. sphaerospermum on solid rice medium yielded three new hybrid polyketides, cladosins L−N (1–3), and a known derivative cladodionen (4). Further cultivation of this fungus on white bean medium afforded an additional new hybrid polyketide, cladosin O (5) along with three known analogues (6–8). The structures of the new compounds were elucidated using a combination of NMR and HRESIMS data. The absolute configurations of compounds 2 and 3 were determined by Mosher’s method and TDDFT-ECD calculations. All isolated compounds were evaluated…
High susceptibility of MDR and XDR Gram-negative pathogens to biphenyl-diacetylene-based difluoromethyl-allo-threonyl-hydroxamate LpxC inhibitors
2016
International audience; Inhibitors of uridine diphosphate-3-O-(R-3-hydroxymyristoyl)-N-acetylglucosamine deacetylase (LpxC, which catalyses the first, irreversible step in lipid A biosynthesis) are a promising new class of antibiotics against Gram-negative bacteria. The objectives of the present study were to: (i) compare the antibiotic activities of three LpxC inhibitors (LPC-058, LPC-011 and LPC-087) and the reference inhibitor CHIR-090 against Gram-negative bacilli (including MDR and XDR isolates); and (ii) investigate the effect of combining these inhibitors with conventional antibiotics.MethodsMICs were determined for 369 clinical isolates (234 Enterobacteriaceae and 135 non-fermentati…
Pseudomonas aeruginosa un Acinetobacter baumanii jutība pret antibakteriālajiem līdzekļiem
2018
Pseudomonas aeruginosa un Acinetobacter baumannii ir galvenie ar veselības aprūpes saistīto infekciju (VASI) izraisītāji. Šo patogēnu rezistence pret antibakteriālajiem līdzekļiem pēdējo gadu laikā ir strauji palielinājusies, un ir viena no aktuālākajām problēmām mūsdienās. Darba mērķis: izpētīt gramnegatīvo baktēriju P. aeruginosa un A. baumannii jutību pret antibakteriālajiem līdzekļiem, salīdzināt antibakteriālās rezistences izmaiņas divu gadu laikā. Uzdevumi: identificēt no elpceļiem iegūtos izolātus ar BBL™ Crystal™ sistēmu, veikt antibakteriālo līdzekļu jutības testus, izmantojot Bauer – Kirby disku difūzijas testu un E – testu, fenotipēšanu veikt pēc antibiogrammām. Rezultātus apko…
Acinetobacter baumannii un Pseudomonas aeruginosa antibakteriālās rezistences noteikšana, lietojot fenotipiskās un molekulāri bioloģiskās metodes
2020
Acinetobacter baumannii un Pseudomonas aeruginosa ir gan Latvijā, gan visā Eiropā bieži sastopami intrahospitāli patogēni, pret kuriem antibiotiku rezistence pieaug ik gadu. Visbiežāk tie skar imūnsupresētus cilvēkus, apdraudot veselību un dzīvību. Darba mērķis: Noteikt A.baumannii un P. aeruginosa jutību pret antibakteriālajiem līdzekļiem 2019. gadā un salīdzināt rezistences izmaiņas ar 2017. gadu, kā arī ar molekulāri bioloģisko metodi identificēt baktēriju sugas. Uzdevumi: 1.No elpceļu paraugiem ar BBL CrystalTM identifikācijas sistēmām identificēt izolātus. 2. Noteikt antimikrobiālo jutību, izmantojot Bauer-KirbyTM disku difūzijas testu un MIC, lietojot ETM-testu. 3. Tipēt izdalīto bakt…
Dissemination of a Carbapenem-Resistant Acinetobacter baumannii Strain Belonging to International Clone II/Sequence Type 2 and Harboring a Novel AbaR…
2013
ABSTRACT An outbreak of hospital-acquired Acinetobacter baumannii infections, caused by a bla OXA-23 -positive carbapenem-resistant strain belonging to international clone II/ST2, was detected in Latvia. The strain was partially equipped with the armA gene and the intI1-aacA4-catB8-aadA1-qacE Δ 1 class 1 integron. In addition, the strain carried AbaR25, a novel AbaR4-like resistance island of ∼46,500 bp containing structures similar to the previously described AbaR22 and Tn 6167 islands. AbaR25 was characterized by the occurrence of a second copy of Tn 6022a interrupted by Tn 2006 carrying the bla OXA-23 gene.
Bacterial Community Structure of an IFAS-MBRs Wastewater Treatment Plant
2017
TIn this work, the bacterial community putatively involved in BNR events of a UCT-MBMBR pilot plant was elucidated by both culture-dependent and metagenomics DNA analyses. The presence of bacterial isolates belonging to Bacillus (in the anoxic compartment) and to Acinetobacter, Stenotrophomonas, Rhodococcus, Escherichia and Aeromonas (in the aerobic compartment) is in agreement with the nitrification/denitrification processes observed in the plant. Moreover, the study of bacterial community structure by NGS revealed a microbial diversity suggesting a biochemical complexity which can be further explored and exploited to improve UCT-MBMBR plant performance.
Presence of pathogenic bacteria in ice cubes and evaluation of their survival in different systems
2017
In this study, 60 samples of ice cubes produced at different levels (domestic, restaurant and industrial facilities), within a restricted geographical area, were investigated for their general microbiological characteristics through the analysis of populations other than enteric bacteria. Total mesophilic bacteria were in the range 1.01 Ã 102â9.55 Ã 103, 3.12 Ã 102â6.31 Ã 103and 1.30 Ã 102â3.99 Ã 103CFU/100Â mL of thawed ice from domestic freezer (DF), stock boxes (SB) for self-production performed with ice machines in bars and pubs, and from sales packages (SP) of industrial productions, respectively. Some DF and SP samples were negative for the presence of total psychrotrophic…