Search results for "Acquisition"
showing 10 items of 822 documents
Effect of video capture time on sperm kinematic parameters in breeding boars
2019
Abstract Computer Assisted Semen Analysis (CASA) technology is being a common tool in the seminal analysis for seminal doses production in farm animals. The aim of the present work was to define the optimal capturing time for a correct evaluation of boar sperm motility. Six sexually mature boars from the Topigs Lines, were used as semen donors. ISASv1 CASA-Mot system was used with an image acquisition rate of 50 Hz for two seconds capturing. Obtained video frames were fragmented into four segments corresponding to 0.5, 1.0, 1.5 and 2.0 s. ISASD4C20 counting chambers, warmed at 37 °C were used. Total and progressive motility were not affected by capture time. Capture time had a significant e…
Tunable-frequency three-dimensional structured illumination microscopy with reduced data-acquisition
2018
The performance of a tunable three-dimensional (3D) structured illumination microscope (SIM) system and its ability to provide simultaneously super-resolution (SR) and optical-sectioning (OS) capabilities are investigated. Numerical results show that the performance of our 3D-SIM system is comparable with the one provided by a three-wave interference SIM, while requiring 40% fewer images for the reconstruction and providing frequency tunability in a cost-effective implementation. The performance of the system has been validated experimentally with images from test samples, which were also imaged with a commercial SIM based on incoherent-grid projection for comparison. Restored images from d…
Label-free quantification in ion mobility-enhanced data-independent acquisition proteomics.
2016
Unbiased data-independent acquisition (DIA) strategies have gained increased popularity in the field of quantitative proteomics. The integration of ion mobility separation (IMS) into DIA workflows provides an additional dimension of separation to liquid chromatography-mass spectrometry (LC-MS), and it increases the achievable analytical depth of DIA approaches. Here we provide a detailed protocol for a label-free quantitative proteomics workflow based on ion mobility-enhanced DIA, which synchronizes precursor ion drift times with collision energies to improve precursor fragmentation efficiency. The protocol comprises a detailed description of all major steps including instrument setup, filt…
The marine activities performed within the TOMO-ETNA experiment
2016
<p>The TOMO-ETNA experiment was planned in order to obtain a detailed geological and structural model of the continental and oceanic crust beneath Mt. Etna volcano and northeastern Sicily up to the Aeolian Islands (southern Italy), by integrating data from active and passive refraction and reflection seismic methodologies, magnetic and gravity surveys. This paper focuses on the marine activities performed within the experiment, which have been carried out in the Ionian and Tyrrhenian Seas, during three multidisciplinary oceanographic cruises, involving three research vessels (“Sarmiento de Gamboa”, “Galatea” and “Aegaeo”) belonging to different countries and institutions. During the o…
Monitoring of system conditioning after blank injections in untargeted UPLC-MS metabolomic analysis
2019
AbstractUltra-performance liquid chromatography – mass spectrometry (UPLC-MS) is widely used for untargeted metabolomics in biomedical research. To optimize the quality and precision of UPLC-MS metabolomic analysis, evaluation of blank samples for the elimination of background features is required. Although blanks are usually run either at the beginning or at the end of a sequence of samples, a systematic analysis of their effect of the instrument performance has not been properly documented. Using the analysis of two common bio-fluids (plasma and urine), we describe how the injection of blank samples within a sequence of samples may affect both the chromatographic and MS detection performa…
Label-Free Proteomics of Quantity-Limited Samples Using Ion Mobility-Assisted Data-Independent Acquisition Mass Spectrometry
2021
Over the past two decades, unbiased data-independent acquisition (DIA) approaches have gained increasing popularity in the bottom-up proteomics field. Here, we describe an ion mobility separation enhanced DIA workflow for large-scale label-free quantitative proteomics studies where starting material is limited. We set a special focus on the single pot solid-phase-enhanced sample preparation (SP3) protocol, which is well suited for the processing of quantity-limited samples.
Towards multilingual competence: examining beliefs and agency in first year university students’ language learner biographies
2021
As working life across the world is increasingly multilingual, multicultural and multidisciplinary, higher education language teaching is faced with a challenge of how to prepare students for it. Many universities have recently developed multilingual pedagogies but central to their success is learners’ perceptions of these practices. To fill this gap, this article explores first year university students’ language learner biographies to gain insight into how learners construct their linguistic realities. The biographies were studied with discourse analytical methods to examine the participants’ beliefs about language learning and their sense of agency in it. The results reveal that participa…
À la recherche du lexique de la gestion hôtelière : de la magdalena de Proust a la WebQuest
2020
This paper describes the creation and design of a series of activities aimed at learning the hotel management lexicon in French within a professional context. The tool used to frame these activities is the WebQuest, based on the principles of socioconstructivism and focused on promoting learning by discovery, among other aims. Accordingly, the action research methodology and after the Webquest implementation with a group of university students taking the French for Specific Purposes subject in a professional context, the study results are presented. Finally, the following conclusions derived from the results obtained allow: (1) to verify the adequacy and effectiveness of the tool created, …
Why Digital Games Can Be Advantageous in Vocabulary Learning
2021
Vocabulary learning is an integral part of language learning; however, it is difficult. Although there are many techniques proposed for vocabulary learning and teaching, researchers still strive to find effective methods. Recently, digital games have shown potentials in enhancing vocabulary acquisition. A majority of studies in digital game-based vocabulary learning (DGBVL) literature investigate the effectiveness of DGBVL tasks. In other words, there are enough answers to what questions in DGBVL literature whereas why questions are rarely answered. Finding such answers help us learn more about the structure of the DGBVL tasks and their effects on vocabulary learning. Hence, to achieve this…
Drawing conclusions about what co-participants know: Knowledge-probing question–answer sequences in new employee orientation lectures
2019
This study aims to uncover the processes of interaction through which knowledge acquisition in new employee orientation is monitored and controlled. Using video-recordings of orientation lectures as data, the study focuses on question–answer sequences in which the lecturer’s question probes into the state of the employees’ knowledge; in particular, it looks at the third turn of the sequence, in which the lecturer comes to a conclusion concerning the participants’ knowledge. This is shown to be an unavoidably practical accomplishment, which is contingent on both the often ambivalent responses of the participants and the design of the knowledge-probing question. Also, the lecturer orients to…