Search results for "Acute"

showing 10 items of 2516 documents

Recombinant human hepatocyte growth factor provides protective effects in cerulein-induced acute pancreatitis in mice.

2018

Acute pancreatitis is a multifactorial disease associated with profound changes of the pancreas induced by release of digestive enzymes that lead to increase in proinflammatory cytokine production, excessive tissue necrosis, edema, and bleeding. Elevated levels of hepatocyte growth factor (HGF) and its receptor c-Met have been observed in different chronic and acute pancreatic diseases including experimental models of acute pancreatitis. In the present study, we investigated the protective effects induced by the recombinant human HGF in a mouse model of cerulein-induced acute pancreatitis. Pancreatitis was induced by 8 hourly administrations of supramaximal cerulein injections (50 µg/kg, ip…

0301 basic medicineMalemedicine.medical_specialtyNecrosisPhysiologyClinical BiochemistryInflammationApoptosismedicine.disease_causeProtective AgentsAntioxidantsProinflammatory cytokine03 medical and health sciencesMiceInternal medicineEdemamedicineAnimalsHumansbusiness.industryHepatocyte Growth FactorCell BiologyProto-Oncogene Proteins c-metmedicine.diseaseGlutathioneSurvival AnalysisRecombinant ProteinsDisease Models AnimalOxidative Stress030104 developmental biologyEndocrinologyPancreatitisAcute pancreatitisPancreatitisHepatocyte growth factormedicine.symptombusinessOxidative stressCeruletidemedicine.drugSignal TransductionJournal of cellular physiology
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Performance evaluation of a newly developed molecular assay for the accurate diagnosis of gastroenteritis associated with norovirus of genogroup II

2018

The performance of a newly proposed fully automated cassette-based sample-to-results solution for norovirus (NoV) detection, InGenius Norovirus ELITe MGB®, was evaluated. A total of 120 selected archival stool samples from children hospitalized for acute gastroenteritis were used to compare the results to a reference real-time RT-PCR. The InGenius NoV assay showed optimal diagnostic accuracy (sensitivity, 100%; specificity, 95.7%) and was able to correctly detect the entire wide panel of epidemiologically relevant genotypes tested. These preliminary results suggest that the InGenius NoV assay can be recommended as a valuable method for accurate diagnosis of NoV GII infection in epidemic and…

0301 basic medicineMalemedicine.medical_specialtySettore MED/07 - Microbiologia E Microbiologia ClinicaAdolescentGenotype030106 microbiologyDiagnostic accuracyBiologymedicine.disease_causeReal-Time Polymerase Chain ReactionSensitivity and SpecificityFeces03 medical and health sciencesMedical microbiologyVirologyGenotypemedicineHumansChildCaliciviridae InfectionsNoroviruCaliciviridae InfectionGastroenteritiReverse Transcriptase Polymerase Chain ReactionNorovirusInfantGeneral MedicineAcute gastroenteritisbacterial infections and mycosesVirologyGastroenteritis030104 developmental biologyCaliciviridae InfectionsFully automatedChild PreschoolNorovirusFeceFemaleHuman
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Inference of SARS-CoV-2 spike-binding neutralizing antibody titers in sera from hospitalized COVID-19 patients by using commercial enzyme and chemilu…

2021

medRxiv: https://doi.org/10.1101/2020.09.07.20188151

0301 basic medicineMalevirusesAntibodies ViralImmunoglobulin Glaw.invention0302 clinical medicineMedical microbiologylawMedicine030212 general & internal medicineNeutralizing antibodyImmunoassaychemistry.chemical_classificationmedicine.diagnostic_testbiologyAntibody titerGeneral MedicineMiddle AgedHospitalizationTiterInfectious DiseasesSpike Glycoprotein CoronavirusOriginal ArticleFemaleAntibodyMicrobiology (medical)Adultmedicine.medical_specialtySevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2)030106 microbiologyNeutralizing antibodiesSensitivity and SpecificityVirusCOVID-19 Serological Testing03 medical and health sciencesEnzyme-linked immunosorbent assayNeutralization TestsHumansAgedChemiluminescenceSARS-CoV-2business.industryfungiCOVID-19Antibodies NeutralizingVirologyChemiluminescent immunoassaysKineticsEnzymechemistryImmunoassayImmunoglobulin Gbiology.proteinbusiness
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Evaluation of Anti-SARS-Cov-2 S-RBD IgG Antibodies after COVID-19 mRNA BNT162b2 Vaccine

2021

(1) Background: The evaluation of anti-spike protein receptor-binding domain (S-RBD) antibodies represents a useful tool to estimate the individual protection against Severe Acute Respiratory Syndrome CoronaVirus 2 (SARS-CoV-2) infection

0301 basic medicineMedicine (General)Coronavirus disease 2019 (COVID-19)Chemiluminescence immunoassayvirusesSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2)Clinical BiochemistryAsymptomaticArticleAntibodies03 medical and health sciencesR5-9200302 clinical medicineMedicineKineticMessenger RNAbiologySARS-CoV-2business.industryVaccinationvirus diseasesCOVID-19spikeVaccination030104 developmental biologyAntibody response030220 oncology & carcinogenesisImmunologybiology.proteinmedicine.symptomAntibodybusinessImmunosurvellianceVaccineS-RBD IgGDiagnostics
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Radioprotection and Radiomitigation: From the Bench to Clinical Practice.

2020

The development of protective agents against harmful radiations has been a subject of investigation for decades. However, effective (ideal) radioprotectors and radiomitigators remain an unsolved problem. Because ionizing radiation-induced cellular damage is primarily attributed to free radicals, radical scavengers are promising as potential radioprotectors. Early development of such agents focused on thiol synthetic compounds, e.g., amifostine (2-(3-aminopropylamino) ethylsulfanylphosphonic acid), approved as a radioprotector by the Food and Drug Administration (FDA, USA) but for limited clinical indications and not for nonclinical uses. To date, no new chemical entity has been approved by …

0301 basic medicineMedicine (miscellaneous)free radicalsReviewPharmacologyFilgrastimGeneral Biochemistry Genetics and Molecular BiologyIonizing radiation03 medical and health sciences0302 clinical medicineSargramostimNew chemical entitymedicinelcsh:QH301-705.5business.industryradioprotectorsAcute Radiation SyndromeAmifostine030104 developmental biologyantioxidantslcsh:Biology (General)Protective Agents030220 oncology & carcinogenesisradiomitigatorsionizing radiationsbusinessPegfilgrastimmedicine.drugBiomedicines
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Time-course of thiol oxidation of protein phosphatases during cerulein-induced acute pancreatitis

2018

Acute pancreatitis (AP) is an acute inflammatory process of the pancreatic gland. The aim of this work was to evaluate the role of thiol oxidation of key proteins that can be involved in the regulation of the inflammatory process during AP. AP was induced in C57BL/6 mice by 7 hourly subcutaneous injections of cerulein (50 ug/kg bw). Animals were sacrificed after 1, 3, 5 and 7 injections of cerulein. One hour after the first injection, hyperoxidation of peroxiredoxin 1–4 was detected coinciding with a H2O2 peak. Three hours later, a marked up-regulation of mRNA and protein expression of sulfiredoxin, partially mediated by Nrf-2, takes place. The up-regulation of sulfiredoxin seems to be resp…

0301 basic medicineMessenger RNA030102 biochemistry & molecular biologyChemistryp38 mitogen-activated protein kinasesThiol oxidationPhosphataseProtein phosphatase 2Pharmacologymedicine.diseaseBiochemistry03 medical and health sciencesSulfiredoxin0302 clinical medicine030220 oncology & carcinogenesisPhysiology (medical)medicineAcute pancreatitisPeroxiredoxinFree Radical Biology and Medicine
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The usefulness of NEWS2 at day 7 of hospitalization in predicting COVID-19 evolution and as an early endpoint in therapeutic trials

2021

Highlights • There is a need for reliable tools to predict the evolution of hospitalized patients suffering from COVID-19 • The likelihood of unfavourable evolution for patients hospitalized for COVID-19 with a National • Early Warning Score 2 (NEWS2) below 7 at Day 7 of hospitalization is nearly non-existent • Such a score could thus be used to allow earlier discharge of the patients and as a judgement criterion in therapeutic trials

0301 basic medicineMicrobiology (medical)2019-20 coronavirus outbreakmedicine.medical_specialtyCoronavirus disease 2019 (COVID-19)Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)030106 microbiologyNEWS2Communicable DiseasesArticle03 medical and health sciences0302 clinical medicineMedicineHumans030212 general & internal medicineIntensive care medicineUnfavourable eventAgedbusiness.industrySARS-CoV-2COVID-19Therapeutic trialCoronavirusHospitalizationInfectious DiseasesItalySARS-CoV2businessPredictionThe Journal of Infection
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Evaluation of a rapid antigen test (Panbio™ COVID-19 Ag rapid test device) for SARS-CoV-2 detection in asymptomatic close contacts of COVID-19 patien…

2021

Objectives There is limited information on the performance of rapid antigen detection (RAD) tests to identify SARS-CoV-2-infected asymptomatic individuals. In this field study, we evaluated the Panbio™ COVID-19 Ag Rapid Test Device (Abbott Diagnostics, Jena, Germany) for this purpose. Methods A total of 634 individuals (355 female; median age, 37 years; range, 9–87) were enrolled. Two nasopharyngeal swabs were collected from household (n = 338) and non-household contacts (n = 296) of COVID-19 cases. RAD testing was carried out at the point of care. The RT-PCR test used was the TaqPath COVID-19 Combo Kit (Thermo Fisher Scientific, MA, USA). Results Household contacts were tested at a median …

0301 basic medicineMicrobiology (medical)AdultMale2019-20 coronavirus outbreakmedicine.medical_specialtyCoronavirus disease 2019 (COVID-19)Rapid antigen detection testAdolescentSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2)Point-of-Care Systems030106 microbiologyTest sensitivityImmunologic TestsAsymptomaticSensitivity and Specificity03 medical and health sciencesYoung Adult0302 clinical medicineCOVID-19 TestingInternal medicineMedicineHumans030212 general & internal medicineChildAntigens ViralAsymptomatic InfectionsAgedAged 80 and overImmunoassayFamily Characteristicsbusiness.industrySARS-CoV-2COVID-19General MedicineMiddle AgedTest (assessment)AsymptomaticResearch NoteInfectious DiseasesRapid antigen testCOVID-19 Nucleic Acid TestingClose contactsFemalemedicine.symptomContact TracingbusinessContact tracingClinical Microbiology and Infection
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One year into the pandemic: Short-term evolution of SARS-CoV-2 and emergence of new lineages.

2021

The COVID-19 pandemic was officially declared on March 11th, 2020. Since the very beginning, the spread of the virus has been tracked nearly in real-time by worldwide genome sequencing efforts. As of March 2021, more than 830,000 SARS-CoV-2 genomes have been uploaded in GISAID and this wealth of data allowed researchers to study the evolution of SARS-CoV-2 during this first pandemic year. In parallel, nomenclatures systems, often with poor consistency among each other, have been developed to designate emerging viral lineages. Despite general fears that the virus might mutate to become more virulent or transmissible, SARS-CoV-2 genetic diversity has remained relatively low during the first ~…

0301 basic medicineMicrobiology (medical)COVID-19 VaccinesSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2)030106 microbiologyReviewBiologyMicrobiologyGenomeVirusDNA sequencing03 medical and health sciencesVACCINESGenetic variationPandemicGeneticsHumansLineagesMolecular BiologyEcology Evolution Behavior and SystematicsTransmission (medicine)SARS-CoV-2VaccinationVariantsGenetic VariationCOVID-19Biological EvolutionVaccination030104 developmental biologyInfectious DiseasesEvolutionary biologyDiagnostic tests
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Ebola Virus Disease Complicated by Late-Onset Encephalitis and Polyarthritis, Sierra Leone

2016

To the Editor: Ebola virus (EBOV) disease is usually an acute illness, but increasing evidence exists of persistent infections and post-Ebola syndromes. We report a case of EBOV encephalitis.

0301 basic medicineMicrobiology (medical)LetterEpidemiologyExpeditedencephalitisviruseslcsh:MedicineEbola virus diseaseLate onsetDiseasemedicine.disease_causepolyarthritislcsh:Infectious and parasitic diseasesSierra LeoneSierra leoneviral persistenceAcute illnessEbola Virus Disease Complicated by Late-Onset Encephalitis and Polyarthritis Sierra LeoneEbola virus03 medical and health sciences0302 clinical medicinemedicinelcsh:RC109-216viruses030212 general & internal medicineLetters to the EditorEbola virusbusiness.industrylcsh:Rvirus diseasesmedicine.diseaseVirology030104 developmental biologyInfectious DiseasesImmunologyPolyarthritisViral persistencebusinessEncephalitisEmerging Infectious Diseases
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