Search results for "Affinity labeling"

showing 3 items of 23 documents

Photoaffinity labeling of the coupling factor 1 from the thermophilic bacterum PS3 by 8-azido ATP

1984

AbstractTo localize the nucleotide binding sites of the F1ATPase (TF1) from the thermophilic bacterium PS3 we have used 14C-labeled 8-azido ATP (8-N3ATP) as photoaffmity label. 8-N3ATP is hydrolyzed by the F,ATPase in the absence of ultraviolet light. Irradiation by ultraviolet light of the enzyme in the presence of 8-N3ATP results in reduction of ATPase activity and in preferential nucleotide specific labeling of the α subunits (0.8–0.9 mol 8-N3ATP/TF1,α:β = 4:1). Inactivation and labeling do not depend on the presence of Mg2+. Both effects decrease upon addition of various nucleotide di- or triphosphates.

chemistry.chemical_classificationPhotoaffinity labelingStereochemistryNoncatalytic nucleotide binding siteThermophileBiophysicsCell BiologyBiochemistryCoupling (electronics)HydrolysisEnzymechemistryStructural BiologyPhotoaffinity labelingMoleBacterial F1ATPaseGeneticsUltraviolet lightCatalytic nucleotide binding siteNucleotideThermophilic bacterium PS3Molecular BiologyFEBS Letters
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Photo-DHEA--a functional photoreactive dehydroepiandrosterone (DHEA) analog.

2011

Abstract The steroid hormone dehydroepiandrosterone (DHEA) has beneficial effects on vascular function, survival of neurons, and fatty acid metabolism. However, a specific receptor for DHEA has not been identified to date. Here, we describe the synthesis of a photoreactive DHEA derivative (Photo-DHEA). In Photo-DHEA, typical characteristics of DHEA are conserved: (i) a “planar” tetracyclic ring system with a Δ 5 double bond, (ii) a 3β-hydroxyl group, and (iii) a keto group at C17. In cell-based assays, Photo-DHEA showed the same properties as DHEA. We conclude that Photo-DHEA is suitable for radioiodination to yield a tool for the identification of the elusive DHEA receptor.

endocrine systemmedicine.medical_specialtyReceptors SteroidDouble bondPhotochemistrymedicine.medical_treatmentClinical BiochemistryDehydroepiandrosteroneBiochemistrychemistry.chemical_compoundEndocrinologyInternal medicinepolycyclic compoundsmedicineHumansskin and connective tissue diseasesReceptorMolecular BiologyG protein-coupled receptorPharmacologychemistry.chemical_classificationFatty acid metabolismPhotoaffinity labelingOrganic ChemistryDHEA receptorDehydroepiandrosteroneSteroid hormoneEndocrinologychemistryMolecular Probeshuman activitieshormones hormone substitutes and hormone antagonistsSteroids
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Insulin-like growth factors in chick embryo retina during development.

1996

Evidence exists supporting an important role for insulin-like growth factors (IGFs) during fetal growth. In the present report we performed studies to define whether developing chick retina contains IGFs and whether IGFs play a role in the growth of this tissue. We have shown that both IGF-I and IGF-II are present in chick embryo retina throughout development (7th-18th day). The highest values, when expressed as ng/g of tissue, were found in the youngest retinas studied (7th-9th day) and at 16th-18th day of development. During whole development the content of IGF-II was about two to three times higher than that ascertained for IGF-I. The tissue also contains cell-surface binding for IGFs. H…

medicine.medical_specialtyPhysiologymedicine.medical_treatmentCellular differentiationClinical BiochemistryChick EmbryoBiologyInsulin-Like Growth Factor ReceptorBiochemistryRetinaInsulin-likeCellular and Molecular NeuroscienceInsulin-like growth factorEndocrinologyInsulin-Like Growth Factor IIInternal medicineCulture TechniquesmedicineAnimalsInsulin-Like Growth Factor IReceptorRetinaAffinity labelingEmbryoCell DifferentiationDNAEndocrinologymedicine.anatomical_structureInsulin-like growth factor 2Culture Media Conditionedbiology.proteinCell DivisionRegulatory peptides
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