Search results for "Agarose gel electrophoresis"

showing 3 items of 13 documents

Separation of Nanoparticles by Gel Electrophoresis According to Size and Shape

2007

We demonstrate the separation of gold and silver nanoparticles according to their size and shape by agarose gel electrophoresis after coating them with a charged polymer layer. The separation is monitored optically using the size- and shape-dependent plasmon resonance of noble metal particles and confirmed by transmission electron microscopy (TEM). Electrophoretic mobilities are quantitatively explained by a model based on the Henry formula, providing a theoretical framework for predicting gel mobilities of polymer coated nanoparticles.

SilverMaterials scienceAnalytical chemistryNanoparticleBioengineeringengineering.materialSilver nanoparticleNanotechnologyComputer SimulationGeneral Materials ScienceParticle SizeSurface plasmon resonancechemistry.chemical_classificationGel electrophoresisMechanical EngineeringGeneral ChemistryPolymerCondensed Matter PhysicsNanostructuresModels ChemicalchemistryAgarose gel electrophoresisengineeringNoble metalComet AssayGoldParticle sizeNano Letters
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The guanidinium group as a key part of water-soluble polymer carriers for siRNA complexation and protection against degradation.

2014

Here, the preparation of a novel block copolymer consisting of a statistical copolymer N-(2-hydroxypropyl) methacrylamide-s-N-(3-aminopropyl) methacrylamide and a short terminal 3-guanidinopropyl methacrylamide block is reported. This polymer structure forms neutral but water-soluble nanosized complexes with siRNA. The siRNA block copolymer complexes are first analyzed using agarose gel electrophoresis and their size is determined with fluorescence correlation spectroscopy. The protective properties of the polymer against RNA degradation are investigated by treating the siRNA block copolymer complexes with RNase V1. Heparin competition assays confirm the efficient release of the cargo in vi…

chemistry.chemical_classificationAcrylamidesMaterials sciencePolymers and PlasticsMicroscale thermophoresisRNase PPolymersOrganic ChemistryWaterFluorescence correlation spectroscopyPolymerchemistry.chemical_compoundchemistryAgarose gel electrophoresisPolymer chemistryEndoribonucleasesMaterials ChemistryCopolymerMethacrylamideMoleculeRNA Small InterferingGuanidineMacromolecular rapid communications
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Degradable cationic nanohydrogel particles for stimuli-responsive release of siRNA.

2014

Well-defined nanogels have become quite attractive as safe and stable carriers for siRNA delivery. However, to avoid nanoparticle accumulation, they need to provide a stimuli-responsive degradation mechanism that can be activated at the payload's site of action. In this work, the synthetic concept for generating well-defined nanohydrogel particles is extended to incorporate disulfide cross-linkers into a cationic nanonetwork for redox-triggered release of oligonucleotide payload as well as nanoparticle degradation under reductive conditions of the cytoplasm. Therefore, a novel disulfide-modified spermine cross-linker is designed that both allows disassembly of the nanogel as well as removal…

chemistry.chemical_classificationMagnetic Resonance SpectroscopyPolymers and PlasticsChemistryOligonucleotideSpermidineOrganic ChemistryCationic polymerizationNanoparticleNanogelsFluorescence correlation spectroscopyHydrogelsPolymerPolyethylene GlycolsNanotoxicologyCationsAgarose gel electrophoresisMaterials ChemistryBiophysicsPolyethyleneimineDisulfidesRNA Small InterferingNanogelMacromolecular rapid communications
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