Search results for "Allomyces"

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Actin in Allomyces arbuscula

1991

International audience; A 42 kDa protein was isolated by affinity chromatography on DNAse I-Sepharose from 24-h-old mycelia of Allomyces arbuscula. It was identified as actin by immunoblots with monoclonal antibody probe against a chicken gizzard actin. This fungal actin has a pI of 5.9 and separates into two spots on two-dimensional polyacrylamide gel electrophoresis, suggesting its dual nature. It can polymerize into 8-10 nm filaments visualized by electron microscopy.

[SDE] Environmental Sciencesmedicine.drug_classAllomyces[SDV]Life Sciences [q-bio]Plant Sciencemacromolecular substancesMonoclonal antibodylaw.invention03 medical and health sciencesAffinity chromatographylawGeneticsmedicine[SDV.BV]Life Sciences [q-bio]/Vegetal Biology[SDV.BV] Life Sciences [q-bio]/Vegetal BiologyPolyacrylamide gel electrophoresisEcology Evolution Behavior and SystematicsActin030304 developmental biologyGel electrophoresis0303 health sciencesbiology030306 microbiologybiology.organism_classificationMolecular biologyActina[SDV] Life Sciences [q-bio]Biochemistry[SDE]Environmental SciencesElectron microscopeBiotechnology

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