Actin in Allomyces arbuscula

6533b854fe1ef96bd12ae067

RESEARCH PRODUCT

Actin in Allomyces arbuscula

D. Van Tuinen B.n. Nguyen Thi Gilbert Turian

subject

[SDE] Environmental Sciences medicine.drug_class Allomyces [SDV]Life Sciences [q-bio]Plant Science macromolecular substances Monoclonal antibody law.invention 03 medical and health sciences Affinity chromatography law Genetics medicine [SDV.BV]Life Sciences [q-bio]/Vegetal Biology [SDV.BV] Life Sciences [q-bio]/Vegetal Biology Polyacrylamide gel electrophoresis Ecology Evolution Behavior and Systematics Actin 030304 developmental biology Gel electrophoresis 0303 health sciences biology 030306 microbiology biology.organism_classification Molecular biology Actina [SDV] Life Sciences [q-bio]Biochemistry [SDE]Environmental Sciences Electron microscope Biotechnology

description

International audience; A 42 kDa protein was isolated by affinity chromatography on DNAse I-Sepharose from 24-h-old mycelia of Allomyces arbuscula. It was identified as actin by immunoblots with monoclonal antibody probe against a chicken gizzard actin. This fungal actin has a pI of 5.9 and separates into two spots on two-dimensional polyacrylamide gel electrophoresis, suggesting its dual nature. It can polymerize into 8-10 nm filaments visualized by electron microscopy.

year	journal	country	edition	language
1991-04-01

https://hal.inrae.fr/hal-02714327

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