Search results for "Alpha"

showing 10 items of 3228 documents

Gamma/delta T lymphocytes are affected in the elderly

2002

gammadelta T lymphocytes are considered to represent a link between the inflammatory response and adaptive immunity. In the present paper we investigated whether these cells play any role in the remodeling of the immune system described in the elderly. We show that the absolute number of gammadelta T cells in peripheral blood of old and very old subjects is reduced. Moreover, gammadelta T cells from old people and centenarians show enhanced levels of the early activation marker CD69 both after culture in medium alone and in LPS-stimulated cells. Furthermore, they show a basal increased production of tumor necrosis factor (TNF)-alpha as evaluated at the single cell level. Additionally, the r…

AdultLipopolysaccharidesMaleAgingT-LymphocytesT cellchemical and pharmacologic phenomenaBiologyLymphocyte ActivationBiochemistryEndocrinologyImmune systemAntigenGeneticsmedicineHumansCytotoxic T cellLymphocyte CountIL-2 receptorMolecular BiologyCells CulturedAgedAged 80 and overTumor Necrosis Factor-alphaLymphokineReceptors Antigen T-Cell gamma-deltahemic and immune systemsCell BiologyMiddle AgedAcquired immune systemmedicine.anatomical_structureImmunologyFemaleTumor necrosis factor alphaExperimental Gerontology
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The relationship between age and production of tumour necrosis factor-α in healthy volunteers and patients with chronic heart failure

2003

Ageing is associated with an altered immune response. Elevated plasma levels of tumour necrosis factor-alpha (TNF-alpha) are present in patients with advanced chronic heart failure (CHF). However, the relationship between age and the immune response in CHF is unknown.We investigated the relationship between age and the TNF-alpha generating capacity of lipopolysaccharide (LPS) stimulated peripheral blood mononuclear cells (PBMC) in nine healthy control subjects (mean age 51.6+/-3.6 years, age range 39-75 years) and 22 stable patients with CHF (mean age 68.3+/-1.5 years, age range 52-78 years, NYHA class 3.0+/-0.2). We also tested the TNF-alpha generating capacity of all control subjects and …

AdultLipopolysaccharidesMalemedicine.medical_specialtyHeart diseaseEnzyme-Linked Immunosorbent AssayPeripheral blood mononuclear cellMonocytesImmune systemInternal medicinemedicineHumansAgedWhole bloodHeart FailureAnalysis of VarianceTumor Necrosis Factor-alphabusiness.industryAge FactorsGestational ageMiddle Agedmedicine.diseaseLogistic ModelsEndocrinologyAgeingCase-Control StudiesHeart failureChronic DiseaseImmunologyFemaleTumor necrosis factor alphaCardiology and Cardiovascular MedicinebusinessBiomarkersInternational Journal of Cardiology
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Effect of noradrenaline and isoproterenol on lipopolysaccharide-induced tumor necrosis factor-alpha production in whole blood from patients with chro…

2005

Increased levels of tumor necrosis factor-alpha (TNF-alpha) correlate with poor prognoses in chronic heart failure (CHF). This study demonstrated that noradrenaline and isoproterenol inhibit TNF-alpha production in patients with CHF in ex vivo whole blood in a dose-dependent fashion. The beta-blocker bisoprolol abolishes this effect.

AdultLipopolysaccharidesMalemedicine.medical_specialtyLipopolysaccharideAdrenergic receptorAdrenergic beta-AntagonistsNorepinephrinechemistry.chemical_compoundInternal medicineReceptors Adrenergic betamedicineBisoprololHumansReceptorWhole bloodHeart FailureTumor Necrosis Factor-alphabusiness.industryIsoproterenolCardiovascular Agentsmedicine.diseaseEndocrinologychemistryBisoprololHeart failurecardiovascular systemCardiologyFemaleTumor necrosis factor alphaCardiology and Cardiovascular MedicinebusinessEx vivomedicine.drugThe American Journal of Cardiology
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Reduced secretion of proinflammatory cytokines of monosodium urate crystal‐stimulated monocytes in chronic renal failure: an explanation for infreque…

2000

In gouty arthritis, monosodium urate (MSU) crystals interact with monocytes and neutrophils to produce inflammatory reactions associated with acute synovitis. In patients with end-stage renal disease (ESRD), gouty arthritis is a rare condition despite often severe hyperuricaemia. We wondered whether differences in the secretion of proinflammatory cytokines by MSU crystal-stimulated monocytes might be one explanation for the low incidence of gouty arthritis in patients with ESRD compared with healthy controls.Thirteen patients with ESRD on intermittent haemodialysis treatment, six patients with chronic renal failure not yet on dialysis, and 15 age- and sex-matched healthy controls were exami…

AdultLipopolysaccharidesMalemusculoskeletal diseasescongenital hereditary and neonatal diseases and abnormalitiesmedicine.medical_specialtyGoutmedicine.medical_treatmentCytological TechniquesInflammationurologic and male genital diseasesProinflammatory cytokinechemistry.chemical_compoundInternal medicinemedicineHumansAgedAged 80 and overTransplantationInterleukin-6Tumor Necrosis Factor-alphabusiness.industryIncidencenutritional and metabolic diseasesMiddle Agedmedicine.diseasePathophysiologyUric AcidGoutEndocrinologyCytokinechemistryNephrologyAcute SynovitisImmunologyCytokinesKidney Failure ChronicUric acidFemaleInflammation Mediatorsmedicine.symptomCrystallizationbusinessInterleukin-1Kidney diseaseNephrology Dialysis Transplantation
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FibroTest is an independent predictor of virologic response in chronic hepatitis C patients retreated with pegylated interferon alfa-2b and ribavirin…

2010

EPIC-3 is a prospective, international study that has demonstrated the efficacy of PEG-IFN alfa-2b plus weight-based ribavirin in patients with chronic hepatitis C and significant fibrosis who previously failed any interferon-alfa/ribavirin therapy. The aim of the present study was to assess FibroTest (FT), a validated non-invasive marker of fibrosis in treatment-naive patients, as a possible alternative to biopsy as the baseline predictor of subsequent early virologic (EVR) and sustained virologic response (SVR) in previously treated patients.Of 2312 patients enrolled, 1459 had an available baseline FT, biopsy, and complete data. Uni- (UV) and multi-variable (MV) analyses were performed us…

AdultLiver CirrhosisMaleBiopsyFibrotestInterferon-alphaAlanine TransaminaseHepatitis C ChronicInterferon alpha-2Middle AgedPrognosisRecombinant ProteinsPolyethylene GlycolsFatty LiverRibavirinHumansDrug Therapy CombinationFemaleProspective Studies
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Lysosomal cathepsins B and L and Stefin A blood levels in patients with hepatocellular carcinoma and/or liver cirrhosis: potential clinical implicati…

1997

The serum levels of lysosomal cathepsin B and L and Stefin A, an intracellular inhibitor of these proteolytic enzymes, were determined in patients with hepatocellular carcinoma (HCC) and/or liver cirrhosis (LC) and correlated with some clinical and biochemical parameters of these diseases. Cathepsin B serum levels were increased in HCC and in LC patients as compared to normal subjects (p < 0.001). However no difference was observed between HCC and LC groups. Interestingly, a significant relationship was evidenced between cathepsin B serum content and the grade of severity of cirrhosis (r = 0.41; p < 0.001). Cathepsin L was significantly elevated only in sera of cancer patients as comp…

AdultLiver CirrhosisMaleCancer Researchmedicine.medical_specialtyPathologyCirrhosisCarcinoma HepatocellularHepatocellular carcinomaoteinase inhibitorCathepsin LLysosomal proteinaseGastroenterologyCathepsin BLiver cirrhosiCathepsin BCathepsin LInternal medicineEndopeptidasesmedicineCarcinomaHumansCystatin AStefin AAgedTumor progression.Aged 80 and overEnzyme PrecursorsbiologyLiver NeoplasmsProteolytic enzymesCancerGeneral MedicineMiddle Agedmedicine.diseaseCathepsinsCystatinsPrCysteine EndopeptidasesOncologyCystatin AHepatocellular carcinomabiology.proteinFemalealpha-FetoproteinsLysosomesOncology
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HCV viraemia is more important than genotype as a predictor of response to interferon in sicily (Southern Italy)

1996

Abstract Background/Aims: To investigate host- and virus-related factors predictive of early and sustained alanine aminotransferase normalization after interferon therapy for HCV-related chronic liver disease, in an area where genotype 1 is highly prevalent. Methods: We studied 100 patients with HCV-RNA positive chronic liver disease (73 chronic hepatitis and 27 cirrhosis) undergoing alpha-interferon treatment. Thirty-four patients had an early response but relapsed, 15 patients remained into sustained response for at least 12 months after therapy, and 51 patients did not respond. Serum HCV-RNA levels were assessed by bDNA (Chiron), and genotype by LiPA (Innogenetics) and by sequencing of t…

AdultLiver CirrhosisMaleCirrhosisGenotypeHepatitis C virusMolecular Sequence DataAlpha interferonChronic liver diseasemedicine.disease_causeAntiviral AgentsGenotypePrevalenceBDNA testmedicineHumansViremiaSicilyInterferon alfaBase SequenceHepatologybusiness.industryInterferon-alphaAlanine TransaminaseHepatitis CMiddle Agedmedicine.diseaseHepatitis CTreatment OutcomeLiverChronic DiseaseImmunologyNucleic Acid ConformationFemalebusinessmedicine.drugJournal of Hepatology
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Raltegravir Pharmacokinetics in Patients on Asunaprevir-Daclatasvir.

2015

ABSTRACT Raltegravir pharmacokinetics was studied in 20 patients included in the ANRS HC30 QUADRIH Study before and after addition of anti-hepatitis C virus (anti-HCV) quadritherapy, including pegylated-interferon–ribavirin and asunaprevir plus daclatasvir. Raltegravir pharmacokinetic parameters remained unchanged whether administered on or off anti-HCV therapy. In addition, concentrations of raltegravir, asunaprevir, and daclatasvir were not affected by liver cirrhosis. These data suggest that in human immunodeficiency virus (HIV)-HCV-coinfected patients, whether cirrhotic or not, asunaprevir and daclatasvir could be administered safely with raltegravir.

AdultLiver CirrhosisMaleDaclatasvirPyrrolidinesAlpha interferonHIV InfectionsHepacivirusPharmacologyAntiviral AgentsRaltegravir PotassiumPolyethylene Glycolschemistry.chemical_compoundPharmacokineticsRaltegravir PotassiumRibavirinMedicineHumansPharmacology (medical)PharmacologySulfonamidesbusiness.industryCoinfectionRibavirinImidazolesvirus diseasesInterferon-alphaValineHepatitis CHepatitis C ChronicMiddle AgedRaltegravirmedicine.diseaseIsoquinolinesdigestive system diseasesRecombinant ProteinsInfectious DiseaseschemistryLiverHIV-1AsunaprevirDrug Therapy CombinationFemaleCarbamatesbusinessmedicine.drugAntimicrobial agents and chemotherapy
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The impact of insulin resistance, serum adipocytokines and visceral obesity on steatosis and fibrosis in patients with chronic hepatitis C.

2007

Aims To assess whether host metabolic factors influence the degree of hepatic steatosis and fibrosis in patients infected with hepatitis C virus, and to evaluate the impact of anti-viral therapy on insulin resistance and serum levels of adipocytokines. Methods Clinical and biochemical features, anthropometrical characteristics, and levels of fasting insulin, leptin, adiponectin and resistin were measured in ‘naı¨ve’ patients with chronic hepatitis C, before, during and after therapy with Peg-Interferon-alpha 2a plus Ribavirin. Results Forty-eightpatientswereincluded(M/F28/20;meanage50.0 12.6years; 62.5% genotype-1). Body mass index was 26.4 4.0 kg/m2 , and visceral obesity was present in 24…

AdultLiver CirrhosisMaleInterferon-alphahcv steatosisHepatitis C ChronicInterferon alpha-2Intra-Abdominal FatMiddle AgedAntiviral AgentsRecombinant ProteinsFatty LiverRibavirinAdipocytesHumansFemaleObesityInsulin ResistanceAlimentary pharmacologytherapeutics
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Circulating, intercellular adhesion molecule-1 in patients with hepatocellular carcinoma

1997

Intercellular adhesion molecule-1 (ICAM-1) is thought to play an important role in cellular immunological reactions. Expression can be induced by inflammatory cytokines in a wide variety of cells, including hepatocytes.To compare the behaviour of ICAM-1 in liver diseases.We assayed serum ICAM-1 (sICAM-1) in patients with hepatocellular carcinoma-associated liver cirrhosis, and compared them with a group of cirrhotic patients and controls. sICAM-1 values were also correlated with some biochemical parameters of liver function. Moreover, immunohistochemical localization of ICAM-1 was performed on liver tissue sections of patients with hepatocellular carcinoma, liver cirrhosis and a sample of n…

AdultLiver CirrhosisMalePathologymedicine.medical_specialtyCarcinoma HepatocellularCirrhosisIntercellular Adhesion Molecule-1Chronic liver diseaseLiver diseasemedicineCarcinomaHumansEndotheliumAgedHepatologymedicine.diagnostic_testbusiness.industryLiver NeoplasmsGastroenterologyAlanine TransaminaseBilirubingamma-GlutamyltransferaseMiddle AgedAlkaline PhosphataseIntercellular Adhesion Molecule-1medicine.diseaseImmunohistochemistryHepatocellular carcinomaFemalealpha-FetoproteinsLiver functionLiver function testsbusiness
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