Search results for "Amebocyte"

showing 3 items of 3 documents

The hemolysin-producer coelomocytes in Holothuria polii

1988

Using sodium metrizoate discontinuous gradients, two hemolysin-producer amebocyte populations have been separated from total circulating Holothuria polii coelomocytes. The amebocytes of population 1 are responsible for the production of the calcium-dependent and temperature-labile hemolysin, whereas those of population 2 produce the calcium-independent and temperature-stable one. The intracytoplasmic hemolysins were evidenced also by immunofluorescence. Petaloid and filipodial amebocytes were the only positive cell types.

Cytotoxicity ImmunologicAmebocyteeducation.field_of_studymedicine.diagnostic_testbiologyImmunologyPopulationTemperatureFluorescent Antibody TechniqueHemolysinImmunofluorescencebiology.organism_classificationMicrobiologyHemolysin ProteinsmedicineAnimalsHolothuriaeducationCoelomocyteEchinodermataDevelopmental BiologyDevelopmental & Comparative Immunology
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Endotoxins in ophthalmic viscosurgical devices.

2003

Purpose To measure the endotoxin concentration (EC) of 25 commercially available, hyaluronic acid- and hydroxypropylmethylcellulose-based (HPMC) ophthalmic viscosurgical devices (OVDs). Methods The in vitro Limulus amebocyte lysate (LAL) assay, which indicates the presence of endotoxins originating from gram-negative bacteria, was used to determine the EC. The procedure was performed according to the European Pharmacopoeia/USP. EC including duplicate determinations, negative controls, dilution series with control standard endotoxin, dilution series with sample extract and positive sample control. Results 16 OVDs (Amvisc®, Amvisc® Plus, Biolon®, Coatel®, Healon®, Healon® GV, Healon®5, HPMC O…

LactoseLimulus testOphthalmologic Surgical ProceduresMethylcelluloseSensitivity and Specificity03 medical and health sciences0302 clinical medicineGram-Negative BacteriaOxazinesHumansConcentration factorHyaluronic AcidLimulus TestEndophthalmitisChromatographyPositive sampleChemistryReproducibility of ResultsGeneral MedicineDilutionEndotoxinsOphthalmologyLimulus amebocyte lysateImmunology030221 ophthalmology & optometry030217 neurology & neurosurgery
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Human endothelial cell-based assay for endotoxin as sensitive as the conventional Limulus Amebocyte Lysate assay

2014

AbstractEndotoxin, also known as lipopolysaccharide (LPS) produced by bacteria can be present in any liquid or on any biomaterial even if the material is sterile. Endotoxin in mammals can cause fever, inflammation, cell and tissue damage and irreversible septic shock and death. In the body, endothelial cells making up the blood vasculature and endothelial cells in vitro rapidly react to minute amounts of endotoxin resulting in a rapid induction of the cell adhesion molecule E-selectin. In this study we have used immunofluorescent staining to evaluate the expression of E-selectin on human microvascular endothelial cells from the skin (HDMEC) and human umbilical vein endothelial cells (HUVEC)…

LipopolysaccharideCellBiophysicsLipopolysaccharideBioengineeringBiologyUmbilical veinEndothelialMicrobiologyBiomaterialschemistry.chemical_compoundEndotoxinLimit of DetectionHorseshoe CrabsmedicineAnimalsHumansCell adhesionCells CulturedCell adhesion moleculeIn vitroEndotoxinsEndothelial stem cellmedicine.anatomical_structurechemistryMechanics of MaterialsLimulus amebocyte lysateCeramics and CompositesLimulus amebocyte assayEndothelium VascularBiomaterials
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