6533b7d3fe1ef96bd1261497
RESEARCH PRODUCT
The hemolysin-producer coelomocytes in Holothuria polii
Calogero CanicattìDonatella CiullaElvira Farina‐liparisubject
Cytotoxicity ImmunologicAmebocyteeducation.field_of_studymedicine.diagnostic_testbiologyImmunologyPopulationTemperatureFluorescent Antibody TechniqueHemolysinImmunofluorescencebiology.organism_classificationMicrobiologyHemolysin ProteinsmedicineAnimalsHolothuriaeducationCoelomocyteEchinodermataDevelopmental Biologydescription
Using sodium metrizoate discontinuous gradients, two hemolysin-producer amebocyte populations have been separated from total circulating Holothuria polii coelomocytes. The amebocytes of population 1 are responsible for the production of the calcium-dependent and temperature-labile hemolysin, whereas those of population 2 produce the calcium-independent and temperature-stable one. The intracytoplasmic hemolysins were evidenced also by immunofluorescence. Petaloid and filipodial amebocytes were the only positive cell types.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1988-09-01 | Developmental & Comparative Immunology |