Search results for "Amide"

The quality of wheat bread with ultrasonicated and fermented by-products from plant drinks production

2021

During plant-based drinks production a significant amount of valuable by-products (BPs) is obtained. The valorization of BPs is beneficial for both the environment and the food industry. The direct incorporation of the fermented and/or ultrasonicated almond, coconut, and oat drinks production BPs in other food products, such as wheat bread (WB) could lead to the better nutritional value as well as quality of WB. Therefore, in this study, various quantities (5, 10, 15, and 20%) of differently treated [ultrasonicated (37 kHz) or fermented with Lacticaseibacillus casei LUHS210] almond, coconut, and oat drinks preparation BPs were used in wheat bread (WB) formulations. Microbiological and other…

Purification and characterization of geranyl diphosphate synthase from Vitis vinifera L. cv Muscat de Frontignant cell cultures

1993

A geranyl diphosphate synthase (EC 2.5.1.1), which catalyzes the formation of geranyl diphosphate from dimethylallyl diphosphate and isopentenyl diphosphate, was isolated from Vitis vinifera L. cv Muscat de Frontignan cell cultures. Purification of the enzyme was achieved successively by ammonium sulfate precipitation and chromatography on DEAE-Sephacel, hydroxylapatite, Mono Q, Phenyl Superose, Superose 12, and preparative nondenaturing polyacrylamide gels. The enzyme formed only geranyl diphosphate as a product. In all cases, neither neryl diphosphate, the cis isomer, nor farnesyl diphosphate was detected. The enzyme showed a native molecular mass of 68 [plus or minus] 5 kD as determined …

Ergosterol elicits oxidative burst in tobacco cells via phospholipase A2 and protein kinase C signal pathway

2004

Ergosterol, a typical fungal sterol, induced in tobacco (Nicotiana tabacum L. cv. Xanthi) suspension cells the synthesis of reactive oxygen species and alkalization of the external medium that are dependent on the mobilization of calcium from internal stores. We used specific inhibitors to elucidate the signal pathway triggered by ergosterol compared with cryptogein, a proteinaceous elicitor of Phytophthora cryptogea. HerbimycinA and genistein, inhibitors of tyrosine protein kinases, had no effect on the oxidative burst and pH changes induced by bothelicitors.Similarly,H-89,aninhibitorofproteinkinaseA,hadnoeffectontheinductionofthesedefensereactions.However,theresponse to both elicitors was…

Soil Solarization and Calcium Cyanamide Affect Plant Vigor, Yield, Nutritional Traits, and Nutraceutical Compounds of Strawberry Grown in a Protected…

2019

Soil solarization is a hydrothermal procedure of disinfesting soil of soilborne diseases and pests. Solarization can be combined with many other chemical or non-chemical alternatives to afford integrated pest and diseases management or improve plant yield. Calcium cyanamide (CaCN2) is a fertilizer used in agriculture sector and is also effective in suppressing soilborne pathogens. The present study assessed the influences of different pre-plant CaCN2 dosages on strawberry grown on solarized or non-solarized soil. Soil solarization and 500 kg ha&minus

In vivoanalysis of the lumenal binding protein (BiP) reveals multiple functions of its ATPase domain

2007

International audience; The endoplasmic reticulum (ER) chaperone binding protein (BiP) binds exposed hydrophobic regions of misfolded proteins. Cycles of ATP hydrolysis and nucleotide exchange on the ATPase domain were shown to regulate the function of the ligand-binding domain in vitro. Here we show that ATPase mutants of BiP with defective ATP-hydrolysis (T46G) or ATP-binding (G235D) caused permanent association with a model ligand, but also interfered with the production of secretory, but not cytosolic, proteins in vivo. Furthermore, the negative effect of BiP(T46G) on secretory protein synthesis was rescued by increased levels of wild-type BiP, whereas the G235D mutation was dominant. U…

Bleaching efficacy of at home 16% carbamide peroxide. A long-term clinical follow-up study.

2019

OBJECTIVE To assess the whitening efficacy of a 16% carbamide peroxidase (CP) gel after 42 months of clinical follow-up. MATERIALS AND METHODS A retrospective clinical study was carried out involving 95 participants. CP 16% was applied for 90 minutes a day for 4 weeks using individualized trays. Tooth color was assessed using spectrophotometry at baseline, 1 week after the end of treatment, and every 6 months until completing 42 months of follow-up. Color parameters were compared using analysis of variance for repeated measures. The analysis by teeth groups was performed using two-way ANOVA. RESULTS The mean baseline L* was 81.7 ± 5.2 versus 83.6 ± 8.8 after 42 months (P<.01). L* showed a d…

Taste Disturbance with Acetazolamide

1997

Système endocannabinoïde : effets sur le métabolisme glucidique, mais aussi lipidique

2016

Resume Le systeme endocannabinoide (SEC) est compose de recepteurs couples aux proteines G : Cannabinoid type 1-receptor (CB 1 -R) et Cannabinoid type 2-receptor (CB 2 -R), de leurs ligands endogenes (anandamide [AEA], et 2-acylglycerol [2-AG]), et des enzymes responsables de leur synthese et de leur degradation. Bien que les recepteurs CB 1 -R et CB 2 -R furent initialement decrits au niveau du systeme nerveux central, ils sont presents dans de nombreux tissus peripheriques, ou ils sont impliques dans le metabolisme, la croissance, et l’inflammation. Apres le retrait commercial du rimonabant, pour des effets secondaires psychiatriques, un agoniste inverse du recepteur CB 1 (CB 1 -R), desti…

Effect of heating method on the microbial levels and acrylamide in corn grits and subsequent use as functional ingredient for bread making

2018

Abstract Cereal by-products represent abundant and low-cost resources of nutrients. The study is dedicated to the development of a rapid method for the treatment of corn grits to obtain value added ingredients for breadmaking. The infrared irradiation (IR) at 76 °C and 90 °C and vacuum microwave (VM) heating at 60 °C have been applied for stabilization of corn grits. The corn grit particles were fractionated by size and it was found that the particle size fraction significantly (p

Bifunctional poly(acrylamide) hydrogels through orthogonal coupling chemistries

2019

Biomaterials for cell culture allowing simple and quantitative presentation of instructive cues enable rationalization of the interplay between cells and their surrounding microenvironment. Poly(acrylamide) (PAAm) hydrogels are popular 2D-model substrates for this purpose. However, quantitative and reproducible biofunctionalization of PAAm hydrogels with multiple ligands in a trustable, controlled, and independent fashion is not trivial. Here, we describe a method for bifunctional modification of PAAm hydrogels with thiol- and amine- containing biomolecules with controlled densities in an independent, orthogonal manner. We developed copolymer networks of AAm with 9% acrylic acid and 2% N-(4…