Search results for "Amplification"
showing 10 items of 258 documents
Experimental parabolic pulse generation with an active dispersion decreasing fiber
2008
We experimentally demonstrate the use of an hybrid configuration to generate parabolic pulses. We combine dispersion decrease with distributed gain. This leads to several benefits on the parabolic generated pulses compared with a passive configuration.
AMPLIFICATION ET CONTROLE TOUT-OPTIQUE DE LA POLARISATION D'UN SIGNAL TELECOM CADENCE A 10 GB/S AU SEIN D'UNE FIBRE OPTIQUE
2012
National audience; Nous démontrons la possibilité de combiner l'amplification Raman et l'attraction de polarisation d'un signal retour-à-zéro cadencé à 10 Gb/s au moyen d'une onde pompe laser contra-propagative dans un unique segment de fibre
Ondes scélérates optiques
2008
International audience; Nous montrons que les systèmes fibrés présentent des caractéristiques remarquables pour la génération et le contrôle d'impulsions rares et intenses similaires aux vagues scélérates océaniques. En particulier, l'amplificateur Raman faiblement dispersif pompé par une onde incohérente a permis de mettre en évidence l'apparition d'ondes scélérates optiques. Un bon accord théorie - expérience est observé.
DNA Extraction From Orthoptera Museum Specimens
2011
We describe a procedure for rapid purification of high quality DNA from either fresh or dry Orthoptera, suitable for the PCR amplification of DNA regions more than 800 bp long (even from oldest specimens), which allows genetic analyses on animals from collections without the complete specimen disruption.
DNA amplification in sea urchin oocytes.
1978
In situ hybridization of ribosomal RNA withParacentrotus lividus ovaries suggests that ribosomal DNA undergoes amplification in the mononucleolate oocytes of this sea urchin.
Coherent Amplification of the Short Optical Pulse in a Non-linear Birefringent Medium
2006
International audience; The propagation of the solitary waves in the resonant birefringent amplifier with linear losses is considered. The birefringent optical linear medium contains two-level atoms with the upper state degenerated over projection of angular moment. It is assumed that population of resonance levels of atoms is inverted. The steady state pulse of polarized radiation that is vectorial generalization of the known pi-pulse was analytically found. Numerical simulations demonstrate the formation dynamics solitary waves originated in birefringent amplifier.
The Status of EGFR Modulates the Effect of miRNA-200c on ZEB1 Expression and Cell Migration in Glioblastoma Cells
2020
Migration of glioblastoma cells into surrounding tissue is one of the main features that makes this tumor incurable. We evaluated whole-genome miRNA expression profiling associated with different EGFR amplification patterns in 30 cases of primary glioblastoma. From the 64 miRNAs that showed differential expression between tumors with a high level of EGFR amplification and tumors without EGFR amplification, 40% were related with cell migration, being miR-200c the most differentially expressed between these two groups. We investigated the effect of miR-200c on ZEB1 expression and cell migration in an in vitro transfection model with a miR-200c mimic, a miR-200c inhibitor and siRNA targeting E…
Validation and application of a PCR primer set to quantify fungal communities in the soil environment by real-time quantitative PCR
2011
Fungi constitute an important group in soil biological diversity and functioning. However, characterization and knowledge of fungal communities is hampered because few primer sets are available to quantify fungal abundance by real-time quantitative PCR (real-time Q-PCR). The aim in this study was to quantify fungal abundance in soils by incorporating, into a real-time Q-PCR using the SYBRGreen (R) method, a primer set already used to study the genetic structure of soil fungal communities. To satisfy the real-time Q-PCR requirements to enhance the accuracy and reproducibility of the detection technique, this study focused on the 18S rRNA gene conserved regions. These regions are little affec…
In-Field LAMP Detection of Flavescence Dorée Phytoplasma in Crude Extracts of the Scaphoideus titanus Vector
2022
One of the most destructive diseases affecting grapevine in Europe is caused by Flavescence Dorée phytoplasma (FDp), which belongs to the 16Sr-V group and is a European Union quarantine pathogen. Although many molecular techniques such as loop-mediated isothermal amplification (LAMP) are widely used for the rapid detection of FDp in infected grapevine plants, there is no developed isothermal amplification assay for FDp detection in the insect vectors that are fundamental for the spread of the disease. For this reason, a simple in-field real-time LAMP protocol was optimized and developed for the specific detection of FDp in the insect vector Scaphoideus titanus. The LAMP assay was optimized …
Multiplex Amplicon Quantification (MAQ), a fast and efficient method for the simultaneous detection of copy number alterations in neuroblastoma
2010
Abstract Background Cancer genomes display characteristic patterns of chromosomal imbalances, often with diagnostic and prognostic relevance. Therefore assays for genome-wide copy number screening and simultaneous detection of copy number alterations in specific chromosomal regions are of increasing importance in the diagnostic work-up of tumors. Results We tested the performance of Multiplex Amplicon Quantification, a newly developed low-cost, closed-tube and high-throughput PCR-based technique for detection of copy number alterations in regions with prognostic relevance for neuroblastoma. Comparison with array CGH and the established Multiplex Ligation-dependent Probe Amplification method…