Search results for "Analysis"
showing 10 items of 26772 documents
Image analysis methods for assessment of H2O2 production and Plasmopara viticola development in grapevine leaves: application to the evaluation of re…
2013
article i nfo The grapevine downy mildew (Plasmopara viticola) provokes severe damages and destroys the harvest in the absence of an effective protection. Numerous fungicide treatments are thus generally necessary. To promote a sustainable production, alternative strategies of protection including new antifungal molecules, resistant geno- types or elicitor-induced resistance are under trial. To evaluate the relevance of these strategies, resistance tests are required. In this context, three image analysis methods were developed to read the results of tests performed to assessP.viticolasporulation and mycelial development, and H 2 O 2 production in leaves. They have been validated using elic…
Analysis of trematode parasite communities in fish eye lenses by pyrosequencing of naturally pooled DNA.
2011
Infections by multiple parasite species are common in nature and have important consequences for between species interactions and coevolutionary dynamics with the host populations. For example ecological and evolutionary factors underlying the structure of parasite communities determine the range of hosts a parasite can infect and set the basis for both evolution of host defences and parasite virulence as well as management of diseases. Studies investigating these factors have been facilitated in the recent past by genetic methods which surmount difficulties of traditional morphological taxonomy in identifying individual parasite species. Here we take a step further and present a novel meth…
Long slide holders for microscope stages
2018
ABSTRACT The use of automated techniques for image analysis of microscopic wood specimens together with new procedures for the preparation of stained xylem tissue support the use of quantitative wood anatomy. These techniques and procedures are especially useful in the studies of retrospective analysis of xylem phenology, reaction(s) of trees to stressful conditions of growth, or reconstruction of long-term growth trends. The unresolved technical problems during the digitalization of cross sections from entire increment cores were stabilization and precise shifting of long microscopic specimens onto the optical microscope stage. For this reason, we have developed a long slide holder for mic…
Cinderella's helping pigeons of the microbial world: The potential of testate amoebae for identifying cryptotephra
2016
Cryptotephra (particles <125μm) is a key record for monitoring past and current volcanic activity. However, its extraction from the host sediment and analysis is often long and difficult because of its small size. Finding a simple method to extract cryptotephra from environmental samples would therefore make its analysis much easier. We hypothesized that arcellinid testate amoebae may hold such a potential. These free-living shelled protists are among the earliest microorganisms to colonize volcanic tephra, and build their shell by agglutinating minerals from their environment. We analyzed by X-ray Spectrometry the mineral signature of tephra from the 2011 Puyehue-Cordon Caulle Volcanic Com…
Phylogenetic evidence for hybrid origins of asexual lineages in an aphid species
2003
International audience; Understanding the mode of origin of asexuality is central to ongoing debates concerning the evolution and maintenance of sexual reproduction in eukaryotes. This is because it has profound consequences for patterns of genetic diversity and ecological adaptability of asexual lineages, hence on the outcome of competition with sexual relatives both in short and longer terms. Among the possible routes to asexuality, hybridization is a very common mechanism in animals and plants. Aphids present frequent transitions from their ancestral reproductive mode (cyclical parthenogenesis) to permanent asexuality, but the mode of origin of asexual lineages is generally not known bec…
Transcriptome and proteome analysis of Pinctada margaritifera calcifying mantle and shell: focus on biomineralization
2010
Abstract Background The shell of the pearl-producing bivalve Pinctada margaritifera is composed of an organic cell-free matrix that plays a key role in the dynamic process of biologically-controlled biomineralization. In order to increase genomic resources and identify shell matrix proteins implicated in biomineralization in P. margaritifera, high-throughput Expressed Sequence Tag (EST) pyrosequencing was undertaken on the calcifying mantle, combined with a proteomic analysis of the shell. Results We report the functional analysis of 276 738 sequences, leading to the constitution of an unprecedented catalog of 82 P. margaritifera biomineralization-related mantle protein sequences. Component…
The phylogeny of termites (Dictyoptera : Isoptera) based on mitochondrial and nuclear markers : implications for the evolution of the worker and pseu…
2008
A phylogenetic hypothesis of termite relationships was inferred from DNA sequence data. Seven gene fragments (12S rDNA, 16S rDNA, 18S rDNA, 28S rDNA, cytochrome oxidase I, cytochrome oxidase II and cytochrome b) were sequenced for 40 termite exemplars, representing all termite families and 14 outgroups. Termites were found to be monophyletic with Mastotermes darwiniensis (Mastotermitidae) as sister group to the remainder of the termites. In this remainder, the family Kalotermitidae was sister group to other families. The families Kalotermitidae, Hodotermitidae and Termitidae were retrieved as monophyletic whereas the Termopsidae and Rhinotermitidae appeared paraphyletic. All of these result…
Bayesian analysis improves experimental studies about temporal patterning of aggression in fish.
2017
Made available in DSpace on 2018-12-11T17:15:13Z (GMT). No. of bitstreams: 0 Previous issue date: 2017-12-01 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) This study aims to describe a Bayesian Hierarchical Linear Model (HLM) approach for longitudinal designs in fish's experimental aggressive behavior studies as an alternative to classical methods In particular, we discuss the advantages of Bayesian analysis in dealing with combined variables, non-statistically significant results and required sample size using an experiment of angelfish (Pterophyllum scalare) species as case study. Groups of 3 individuals were subjected to daily observations recorded for 10 min durin…
Reappraisal of ‘chronospecies' and the use of Arvicola (Rodentia, Mammalia) for biochronology.
2008
13 pages; International audience; The water vole, genus Arvicola, is characterised by a broad geographic distribution throughout Europe and is widespread during the late Middle and Upper Pleistocene. This genus is used as a major biostratigraphic tool within the Quaternary. Specific determinations using the Schmelzband-Differenzierung-Quotient or SDQ have identified many chronospecies within the fossil species Arvicola cantiana (Hinton, 1910). As SDQ calculation remains limited, this study reappraises the Arvicola genus in terms of morphodiversity and morphospace using outline analysis which takes into account the tooth as a whole. Outline analysis suggests that one single species of Arvico…
Microsatellite analysis of red mullet Mullus barbatus (Perciformes, Mullidae) reveals the isolation of the Adriatic Basin in the Mediterranean Sea
2009
AbstractMaggio, T., Lo Brutto, S., Garoia, F., Tinti, F., and Arculeo, M. 2009. Microsatellite analysis of red mullet Mullus barbatus (Perciformes, Mullidae) reveals the isolation of the Adriatic Basin in the Mediterranean Sea. – ICES Journal of Marine Science, 66: 1883–1891. The red mullet Mullus barbatus is commercially one of the most important demersal fish resources in the Mediterranean. Molecular data on its genetic population structure throughout the Mediterranean are reported. Six microsatellite loci displayed a high degree of expected heterozygosity and a high allele number per locus. The Hardy–Weinberg equilibrium test revealed an overall tendency towards heterozygote deficiency, …