6533b850fe1ef96bd12a850e
RESEARCH PRODUCT
Image analysis methods for assessment of H2O2 production and Plasmopara viticola development in grapevine leaves: application to the evaluation of resistance to downy mildew.
Charles SchneiderMarielle AdrianMarie-claire HéloirXavier DaireIan Li Kim KhiookBenjamin EboisSophie Trouvelotsubject
0106 biological sciencesMicrobiology (medical)Antifungalmedicine.drug_class[SDV]Life Sciences [q-bio]H2O2Context (language use)01 natural sciencesMicrobiologyImage analysis03 medical and health sciencesPlasmopara viticolamedicinePlant defense against herbivoryImage Processing Computer-Assisted[SDV.BV]Life Sciences [q-bio]/Vegetal BiologyVitisimage analysis;Plasmopara viticola;downy mildew;grapevine;H2O2;resistance testsMolecular Biology[ SDV.MP.MYC ] Life Sciences [q-bio]/Microbiology and Parasitology/MycologyAnalysis method[SDV.MP.MYC]Life Sciences [q-bio]/Microbiology and Parasitology/Mycology030304 developmental biologyDisease ResistancePlant Diseases2. Zero hunger0303 health sciencesResistance (ecology)biologyResistance testsReproducibility of Resultsfood and beveragesHydrogen Peroxidebiology.organism_classificationFungicidePlant LeavesHorticultureAgronomyOomycetesPlasmopara viticola[SDE]Environmental SciencesDowny mildewGrapevine010606 plant biology & botanyDowny mildewdescription
article i nfo The grapevine downy mildew (Plasmopara viticola) provokes severe damages and destroys the harvest in the absence of an effective protection. Numerous fungicide treatments are thus generally necessary. To promote a sustainable production, alternative strategies of protection including new antifungal molecules, resistant geno- types or elicitor-induced resistance are under trial. To evaluate the relevance of these strategies, resistance tests are required. In this context, three image analysis methods were developed to read the results of tests performed to assessP.viticolasporulation and mycelial development, and H 2 O 2 production in leaves. They have been validated using elicitors of plant defenses. These methods are reliable, innovative, rapid, and their modular concept allows their further adaptation to other host-pathogen systems. © 2013 Elsevier B.V. All rights reserved.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2013-11-01 |