Search results for "Anatomia"

CK2β-regulated signaling controls B cell differentiation and function

2023

Serine-Threonine kinase CK2 supports malignant B-lymphocyte growth but its role in B-cell development and activation is largely unknown. Here, we describe the first B-cell specific knockout (KO) mouse model of the β regulatory subunit of CK2. CK2βKO mice present an increase in marginal zone (MZ) and a reduction in follicular B cells, suggesting a role for CK2 in the regulation of the B cell receptor (BCR) and NOTCH2 signaling pathways. Biochemical analyses demonstrate an increased activation of the NOTCH2 pathway in CK2βKO animals, which sustains MZ B-cell development. Transcriptomic analyses indicate alterations in biological processes involved in immune response and B-cell activation. Upo…

SVILUPPO DI METODICHE PER LA DIAGNOSI DELLA BABESIOSI BOVINA E RICERCA DI CANDIDATI VACCINALI IN BABESIA BIGEMINA

2012

Cattle babesiosis is a tick-borne disease transmitted by haemoparasites belonging to the phylum of Apicomplexa, such as Babesia bovis and B.bigemina. The pathology affects cattle mainly in tropical and subtropical areas, but also in Europe, strongly reducing meat and milk production. The study performed during these three years of PhD was addressed to the molecular characterization of Italian B.bigemina strains isolated from infected animals, with attention to the genes codifying for surface antigens, putative candidates for vaccine and diagnostic tools development. One of the molecules potentially involved in the erythrocyte invasion by B.bigemina is the Apical Membrane Antigen-1 (AMA-1), …

Production of high value added nutraceuticals in a multi-trophic aquaculture system within a closed circuit marine hatchery (NUTRAQUA)

2022

Estudio anatómico-histológico de las flores del endemismo Lamottea diania (Asteraceae) y de los efectos del ozono troposférico sobre su desarrollo.

2016

"Estudio anatómico-histológico de las flores del endemismo Lamottea diania (Asteraceae) y de los efectos del ozono troposférico sobre su desarrollo". Lilloa 53 (2). Plantas del endemismo Lamottea diania se expusieron en cámaras OTC (Open Top Chambers) a aire ambiente filtrado y a aire ambiente no filtrado más 30 ppb de ozono para observar el efecto de este contaminante sobre el desarrollo de sus flores, particularmente sobre su androceo. Hemos comprobado, mediante estudios de microscopía óptica y electrónica, que el ozono afecta el proceso de desarrollo y maduración de los estambres y del polen. Las anteras se ven afectadas, en unos casos al abortar algunos estambres en su desarrollo y, en …

Lever Press Duration as a Measure of Frustration Motivated Response Variation in Rat Self-administration to Investigate the Effects of Frustration on…

2023

In human studies, frustration has been identified as a potential risk factor for substance use disorders. Currently, there is little research into the role of frustration in substance use disorders despite research showing that frustration tolerance in humans is associated with a lower likelihood of developing substance use problems, better outcomes in recovery, and fewer relapses. To address this need, our studies use rat self-administration models to focus on frustration-related behavior in natural reward and addiction-related behavioral procedures. First, to study frustration in operant responding, there is a need to establish a real-time objective measure and validate its use in predict…

Effect of conditioned media from osteo- and adipo-differentiating mesenchymal stem cells on triple negative MDA-MB231 breast cancer cells

2015

It is know that mesenchymal stem cells (MSCs) actively secretemultiple biologically-active factors during their process of differentiation which gives rise to a variey of cytotypes including bone and fatcells. It is also acknowledged that the chemokines secreted throughoutMSC differentiation may play an important role in the development and growth of tumor cells, although literature data appear somewhat indeterminate due to the contradictory evidence often found.

Gene ontology-based annotation and comparative analysis of proteins extracted from proteomics of 100 breast cancer patients.

2009

Background: Current clinical parameters for breast cancer diagnosis and therapy are: tumour size, axillary lymph node status, histological grading and presence or absence of metastases. Prognostic/predictive properties, such as oestrogen and progesterone receptor status, and human epidermal growth factor receptor (HER-2/neu) status are currently used for therapeutic decision. Conversely, it is now emerging that the number of genetic mutations and epigenetic deregulations in cancer is far more higher than previously thought. Therefore, proteomic screening for differential protein expression in subsets of tumor samples is an essential tool for generating data bases and biomarker discovery. Th…

Introduzione a Biologia dello Sviluppo. Principi generali dello Sviluppo.

2010

Con il termine “sviluppo” ci riferiamo fondamentalmente all’insieme degli eventi attraverso cui da un uovo fecondato si genera un organismo pluricellulare, composto da differenti tipi cellulari, organizzati in tessuti ed organi, ciascuno deputato a svolgere una specifica funzione (di sostegno, di protezione, di nutrizione, di locomozione, sensoriale, riproduttiva, ecc.) e con una sua ben precisa forma tridimensionale e localizzazione spaziale rispetto alle altre strutture che compongono l’organismo.

Interactive effects of increased temperature and gadolinium pollution in Paracentrotus lividus sea urchin embryos: a climate change perspectiveIntera…

2021

Gradual ocean warming and marine heatwaves represent major threats for marine organisms already facing other anthropogenic-derived hazards, such as chemical contamination in coastal areas. In this study, the combined effects of thermal stress and exposure to gadolinium (Gd), a metal used as a contrasting agent in medical imaging which enters the aquatic environment, were investigated in the embryos and larvae of the sea urchin Paracentrotus lividus. Embryos were exposed to six treatments of three temperatures (18 °C, 21 °C, 24 °C) and two Gd concentrations (control: 0 μM; treated: 20 μM). With respect to developmental progression, increased temperature accelerated development and achievemen…

Effects of BDE-47 exposure on immune-related parameters of Mytilus galloprovincialis.

2019

Abstract The persistent pollutants polybrominated diphenyl ethers (PBDEs) have been demonstrated to produce several negative effects on marine organisms. Although Mytilus galloprovincialis was extensively studied as model system, the effects of PBDEs on the innate immune system of mussels remains unclear. In this study, except for the control treatment, specimens of M. galloprovincialis were fed with microalgae treated with increasing concentrations of PBDEs (maximum level 100 ng L−1 of BDE-47 per day). BDE-47 treatment was maintained for 15 days and then the animals were fed with the same control diet, without contaminants, for 15 days. Samples of haemolymph (HL) were obtained at T0, T15 a…