Search results for "Andrology"
showing 10 items of 410 documents
Double-application of platelet-rich plasma on bone healing in rabbits
2010
Objective: Platelet-rich plasma (PRP) is considered to enhance bone formation especially at early stages of wound healing, depending on the limited and short life-span of platelets and growth factors. The aim of this study was to evaluate efficacy of double-application of PRP (DA-PRP) on bone healing in a rabbit calvarial defect model. Study design: Twenty-eight rabbits, each had two surgically prepared calvarial bone defects (10mm diameter), were included in this study and randomly divided into six groups. Defects (n=56) were treated with single-application of PRP (SA-PRP)(n=10), SA-PRP and beta-tricalciumphosphate (SA-PRP+TCP)(n=10), DA-PRP (n=8), DA-PRP and beta-tricalciumphosphate (DA-P…
Osmotic and cryoprotective effects of a mixture of DMSO and ethylene glycol on rabbit morulae.
1993
Abstract Comparisons were made of the osmotic and cryoprotective effects on rabbit embryos preserved by vitrification with 2 solutions and by conventional freezing. Embryos obtained from rabbits killed 70 to 72 h after mating were used in the study (n = 948). Initially, toxicity of the 3 cryoprotectants was studied in fresh (unfrozen) embryos (n = 135). Subsequently, embryos placed in ethylene glycol (EG, 40% v/v; n = 88) and ethylene glycol with dimethyl sulfoxide (EG+DMSO, 20% v/v each, respectively; n = 344) were loaded into straws and plunged directly into liquid nitrogen. Embryos placed in 1.5 M DMSO and 20% heat inactivated rabbit serum were subjected to conventional freezing in a pro…
Direct transfer of vitrified rabbit embryos.
1994
In this study we looked at the feasibility of transferring vitrified rabbit embryos directly into recipient does. Compacted morulae were vitrified in a solution of 20% ethylene glycol and 20% dimethyl sulfoxide. After thawing, and without step-wise diluted solution, the vitrified embryos were transferred into the recipient's uterine horns. Survival rate at birth differed from fresh rabbit embryos (40% vs 55%, P0.05). However, the percentage of does that delivered (94%) and the survival rate suggested this method is suitable for both storage and simple transfer of rabbit morulae.
Cytochrome P450 1A- and stress protein-induction in early life stages of medaka (Oryzias latipes) exposed to trichloroethylene (TCE) soot and differe…
2013
It has previously been shown that trichloroethylene (TCE) soot extracts cause dioxinlike toxic effects in medaka fish (Oryzias latipes) and primary liver cell culture of rainbow trout (Oncorhynchus mykiss). This study examines embryonic and larval induction of cytochrome P450 1A and stress proteins after exposure of medaka embryos to extracts and fractions of TCE combustien-generated aerosols. Embryos were exposed to three concentrations of whole soot extract (WE; 2.7, 7.2 and 18 μg l -1 incomplete combustion by-products), TCDD (2,3,7,8-tetrachlorodibenzo-p-dioxin, 3 ng l -1 ) and four TCE fractions with different polarity (Fr 1-4; 18 μg l -1 ) for 8 days. Approximately 50% of the embryos w…
Retinoid dynamics in chicken eye during pre- and postnatal development.
1994
Changes in the steady state level of retinols, retinaldehydes and retinyl esters in the trans and 11-cis forms and trans retinoic acid were measured in whole chicken eye during development from day 6 in ovo to day 3 post-hatch. These retinoids, quantified by different HPLC systems, were detected in this time sequence: trans-retinol and trans-retinyl esters in the first week in ovo, 11-cis-retinol in the second week. The highest level of 11-cis-retinaldehyde and 11-cis-retinyl esters was reached at the end of development in ovo; however, their levels increased further after hatching. The retinoic acid level decreased at the end of the first week, rising again at the end of the second week. T…
Beneficial Effect of Two Culture Systems with Small Groups of Embryos on the Development and Quality of In Vitro-Produced Bovine Embryos
2014
Summary Currently, in vitro-produced embryos derived by ovum pick up (OPU) and in vitro fertilization (IVF) technologies represent approximately one-third of the embryos worldwide in cattle. Nevertheless, the culture of small groups of embryos from an individual egg donor is an issue that OPU-IVF laboratories have to face. In this work, we tested whether the development and quality of the preimplantation embryos in vitro cultured in low numbers (five embryos) could be improved by the addition of epidermal growth factor, insulin, transferrin and selenium (EGF-ITS) or by the WOW system. With this aim, immature oocytes recovered from slaughtered heifers were in vitro matured and in vitro ferti…
Modulation of immune parameters of roach, Rutilus rutilus, exposed to untreated ECF and TCF bleached pulp effluents
2000
Abstract The present study was designed to assess the effects of elemental chlorine free (ECF) and totally chlorine free (TCF) pulp and paper mill effluents on the immune defence of the roach ( Rutilus rutilus ). Fish were exposed for 5 weeks to concentrations of 0, 0.6, 2, 6 and 20% of untreated effluents from ECF or TCF bleaching processes. In order to study the capability to respond to foreign antigens the fish were immunised with bovine γ-globulin (BGG) 3 weeks before sampling. The numbers of anti-BGG antibody-secreting cells and immunoglobulin-secreting cells in the spleen and blood, as well as the levels of the anti-BGG antibodies and concentrations of immunoglobulin in plasma and the…
Knock out der c-Jun N-terminalen Kinase 2 (JNK2) aggraviert die Entwicklung der chronischen DSS-Colitis unabhängig von der intestinalen Zytokin-Expre…
2008
Background The c-Jun N-terminal kinase 2 (JNK2) is involved in signal transduction of inflammatory bowel diseases (IBD) and regulates the expression of pro-inflammatory cytokines. For this reason, JNK2 is considered as novel target for IBD therapy. The aim of this study was 1.) to examine the function of JNK2 applying a low dose Dextran Sulfate Sodium (DSS) model of chronic experimental colitis in JNK2 knock out mice and 2.) to analyze the expression of JNK2 dependent cytokines. Material and Methods: For induction of a mild chronic colitis, female JNK2 knockout mice (JNK2 ko) and their wildtype controls (WT2) received three cycles of DSS treatment, each consisting of 1.0 % DSS for 5 days, f…
Ability of the ISAS3Fun Method to Detect Sperm Acrosome Integrity and Its Potential to Discriminate between High and Low Field Fertility Bulls
2021
The objective of the present study was to investigate whether fertility differences in bulls are reflected in variations of sperm quality when analysing only one ejaculate per male. Two experiments were performed. In the first experiment, frozen semen samples from 20 adult bulls were tested
The use of morphokinetics as a predictor of embryo implantation.
2011
Background Time-lapse observation presents an opportunity for optimizing embryo selection based on morphological grading as well as providing novel kinetic parameters, which may further improve accurate selection of viable embryos. The objective of this retrospective study was to identify the morphokinetic parameters specific to embryos that were capable of implanting. In order to compare a large number of embryos, with minimal variation in culture conditions, we have used an automatic embryo monitoring system. Methods Using a tri-gas IVF incubator with a built-in camera designed to automatically acquire images at defined time points, we have simultaneously monitored up to 72 individual emb…