Search results for "Animal Structures"
showing 10 items of 876 documents
L'incostituzionalità della diagnosi genética preimpianto
2015
medically assisted procreation, preimplantation genetic diagnosis, embryo
Two Latent and Two Hyperstable Polymeric Forms of Human Neuroserpin
2010
AbstractHuman neuroserpin (hNS) is a serine protease inhibitor that belongs to the serpin superfamily and is expressed in nervous tissues. The serpin fold is generally characterized by a long exposed loop, termed the reactive center loop, that acts as bait for the target protease. Intramolecular insertion of the reactive center loop into the main serpin β-sheet leads to the serpin latent form. As with other known serpins, hNS pathological mutants have been shown to accumulate as polymers composed of quasi-native protein molecules. Although hNS polymerization has been intensely studied, a general agreement about serpin polymer organization is still lacking. Here we report a biophysical chara…
Isolation and characterization of haemoporin, an abundant haemolymph protein from Aplysia californica.
2003
In the present study, we show the isolation and characterization of the protein haemoporin, which constitutes the second most abundant protein fraction in the haemolymph of the marine gastropod Aplysia californica. Although Aplysia is commonly used to investigate the molecular basis of learning, not much is known about the proteins in its haemolymph, which is in contact with the neurons owing to the open circulatory system of molluscs. In the native state, haemoporin is a macromolecular complex forming a cylinder with a central solvent-filled pore. The native complex most probably is a homopentamer made up from 70 kDa subunits with a molecular mass of 360 kDa and a sedimentation coefficient…
Methylation Reprogramming and Chromosomal Aneuploidy in In Vivo Fertilized and Cloned Rabbit Preimplantation Embryos1
2004
Active demethylation of the paternal genome but not of the maternal genome occurs in fertilized mouse, rat, pig, and bovine zygotes. To study whether this early demethylation wave is important for embryonic development, we have analyzed the global methylation patterns of both in vivo-fertilized and cloned rabbit embryos. Anti-5-methylcytosine immunofluorescence of in vivo-fertilized rabbit embryos revealed that the equally high methylation levels of the paternal and maternal genomes are largely maintained from the zygote up to the 16-cell stage. The lack of detectable methylation changes in rabbit preimplantation embryos suggests that genome-wide demethylation is not an obligatory requireme…
American cockroach control assays in the municipal sewerage system of Valencia (Spain)
2013
ABSTRACT Several insecticides (including different active substances and formulations) were applied to sewer shafts to control Periplaneta americana (LINNAEUS, 1758) populations in Valencia (eastern Spain). Results show that the best products for cockroach control were the paints (with and without insecticide), which provided an optimal reduction of P. americana populations for up to three months (P < 0.001). Therefore, it is important to note that the reduction of cockroach populations as well as the persistence of the insecticide effect in sewers was similar in both types of paints. A product combining two pyrethroids (Tetramethrin and Alfacipermetrin) and piperonyl butoxide also gave …
FlyMove – a new way to look at development of Drosophila
2003
Development of any organism requires a complex interplay of genes to orchestrate the many movements needed to build up an embryo. Previously, work on Drosophila melanogaster has provided important insights that are often applicable in other systems. But developmental processes, which take place in space and time, are difficult to convey in textbooks. Here, we introduce FlyMove (http://flymove.uni-muenster.de), a new database combining movies, animated schemata, interactive "modules" and pictures that will greatly facilitate the understanding of Drosophila development.
Dynamics of thermally induced optical nonlinearity in GaSe thin slabs
1996
A study of the nonlinear effects shown by thin slabs of GaSe metaled with Au is presented.
Functional consequences of prey acclimation to ocean acidification for the prey and its predator
2016
Ocean acidification is the suite of chemical changes to the carbonate system of seawater as a consequence of anthropogenic carbon dioxide (CO2) emissions. Despite a growing body of evidences demonstrating the negative effects of ocean acidification on marine species, the consequences at the ecosystem level are still unclear. One factor limiting our ability to upscale from species to ecosystem is the poor mechanistic understanding of the functional consequences of the observed effects on organisms. This is particularly true in the context of species interactions. The aim of this work was to investigate the functional consequence of the exposure of a prey (the mussel Brachidontes pharaonis) t…
Functional consequences of prey acclimation to ocean acidification for the prey and its predator
2015
Ocean acidification is the suite of chemical changes to the carbonate system of seawater as a consequence of anthropogenic carbon dioxide (CO2) emissions. Despite a growing body of evidences demonstrating the negative effects of ocean acidification on marine species, the consequences at the ecosystem level are still unclear. One factor limiting our ability to upscale from species to ecosystem is the poor mechanistic understanding of the functional consequences of the observed effects on organisms. This is particularly true in the context of species interactions. The aim of this work was to investigate the functional consequence of the exposure of a prey (the mussel Brachidontes pharaonis) t…
Hsp60 Inhibitors and Modulators
2019
In this chapter, we focus on the 60 KDa Heat Shock Protein (Hsp60) and discuss some of its biological, molecular and pathological features. The structural and mechanistic aspect of the Hsp60 folding cycle will be also presented. We further illustrate how Hsp60 may be involved in many diseases and therefore considered as an effective therapeutic or theranostic target. Finally, the state-of-the-art on the development of Hsp60 and bacterial GroEL inhibitors and modulators of their expression will be illustrated. This is discussed in the light of a negative chaperonotherapy, and the consequent development of inhibitors, as well as positive chaperonotherapy, in the event its excessive activity i…