Search results for "Antigen processing"

showing 10 items of 71 documents

RAPID AND EFFICIENT ANTIGEN PROCESSING AND PRESENTATION OF A PROTECTIVE AND IMMUNODOMINANT HLA-B*27-RESTRICTED HEPATITIS C VIRUS-SPECIFIC CD8+T CELL …

2012

HLA-B*27 exerts protective effects in hepatitis C virus (HCV) and human immunodeficiency virus (HIV) infections. While the immunological and virological features of HLA-B*27-mediated protection are not fully understood, there is growing evidence that the presentation of specific immunodominant HLA-B*27-restricted CD8+ T-cell epitopes contributes to this phenomenon in both infections. Indeed, protection can be linked to single immunodominant CD8+ T-cell epitopes and functional constraints on escape mutations within these epitopes. To better define the immunological mechanisms underlying HLA-B*27-mediated protection in HCV infection, we analyzed the functional avidity, functional profile, ant…

MaleProteasome Endopeptidase ComplexQH301-705.5Immune CellsAntigen presentationImmunologyAntigen-Presenting CellsAntigen Processing and RecognitionHepacivirusBiologyAdaptive ImmunityCD8-Positive T-LymphocytesMicrobiologyEpitopeImmune ActivationMajor Histocompatibility ComplexEpitopesImmune systemVirologyGeneticsCytotoxic T cellHumansAvidityBiology (General)Antigen-presenting cellMolecular BiologyBiologyAntigen PresentationLinear epitopeAntigen processingT CellsImmunityRC581-607VirologyHepatitis CHLA-B AntigensImmunologyProteolysisQR180ParasitologyFemaleImmunologic diseases. AllergyHepatitis C AntigensResearch Article
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Clinical efficacy of blue light full body irradiation as treatment option for severe atopic dermatitis.

2011

Background Therapy of atopic dermatitis (AD) relies on immunosuppression and/or UV irradiation. Here, we assessed clinical efficacy and histopathological alterations induced by blue light-treatment of AD within an observational, non-interventional study. Methodology/Principal Findings 36 patients with severe, chronic AD resisting long term disease control with local corticosteroids were included. Treatment consisted of one cycle of 5 consecutive blue light-irradiations (28.9 J/cm2). Patients were instructed to ask for treatment upon disease exacerbation despite interval therapy with topical corticosteroids. The majority of patients noted first improvements after 2–3 cycles. The EASI score w…

MaleSkin PhysiologyAnatomy and PhysiologyLightmedicine.medical_treatmentlcsh:MedicinePediatric DermatologyAntigen Processing and RecognitionAtopic DermatitisDiseasePediatricsQuality of lifeYoung adultlcsh:ScienceSkinMultidisciplinarymedicine.diagnostic_testT CellsAllergy and HypersensitivityPhysicsElectromagnetic RadiationPhotodermatology and Skin AgingImmunosuppressionAtopic dermatitisMiddle Agedmedicine.anatomical_structureTreatment OutcomePatient SatisfactionObservational StudiesMedicineFemaleWhole-Body IrradiationResearch ArticleAdultmedicine.medical_specialtyLangerhans cellClinical Research DesignImmune CellsImmunologyColorDermatologyDermatitis AtopicYoung AdultUltraviolet RadiationBiopsymedicineHumansSerologic TestsBiologybusiness.industrylcsh:Rmedicine.diseaseDermatologyClinical trialImmune SystemChronic DiseaseQuality of Lifelcsh:QClinical ImmunologybusinessPloS one
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Immunoproteasome LMP2 60HH Variant Alters MBP Epitope Generation and Reduces the Risk to Develop Multiple Sclerosis in Italian Female Population

2010

BackgroundAlbeit several studies pointed out the pivotal role that CD4+T cells have in Multiple Sclerosis, the CD8+ T cells involvement in the pathology is still in its early phases of investigation. Proteasome degradation is the key step in the production of MHC class I-restricted epitopes and therefore its activity could be an important element in the activation and regulation of autoreactive CD8+ T cells in Multiple Sclerosis.Methodology/principal findingsImmunoproteasomes and PA28-alphabeta regulator are present in MS affected brain area and accumulated in plaques. They are expressed in cell types supposed to be involved in MS development such as neurons, endothelial cells, oligodendroc…

MaleT cells proteasomes multiple sclerosis parietal lobeMuscle ProteinsImmunoproteasomeEpitopeEpitopesGene FrequencyRisk FactorsCytotoxic T cellFunding: This work was financed in part by the grant Giovani Ricercatori 2007 from Italian Ministry of Health to MM DG and FMB by a grant from the European Commission Integrated Project PROTEOMAGE (FP6) to CF by the finalized projects of Fondazione Italiana Sclerosi Multipla (FISM) cod. 2003/R26 and BioPharmaNet to CF and 2002/R/40 and 2005/R/10 2008/R/11 (Genoa) to SD'A by the University of Bologna (FRO) to MPF by the Regione Piemonte (Ricerca Sanitaria Finalizzata Project and Ricerca Sanitaria Applicata-CIPE Project) to SD'A by Associazione Amici del Centro Dino Ferrari and IRCCS Ospedale Maggiore Policlinico Milano to DG and by the grants Sonderforschungsbereich (SFB-507 SFB-421) to PMK and US the grants TR43 and Neurocure to PMK. MM benefited from the A.V. Humboldt PostDoc fellowship. The funders had no role in study design data collection and analysis decision to publish or preparation of the manuscript.MultidisciplinaryMicrogliaQRBrainMiddle AgedImmunohistochemistryCysteine EndopeptidasesOligodendrogliamedicine.anatomical_structureItalyImmunoproteasome; multiple sclerosis; italian populationmultiple sclerosiImmunology/Antigen Processing and RecognitionMedicineFemaleMicrogliaNeuroscience/Neurobiology of Disease and RegenerationResearch ArticleProtein BindingAdultProteasome Endopeptidase ComplexMultiple SclerosisGenotypeScienceMolecular Sequence DataImmunology/AutoimmunityBiologySex FactorsMHC class IHLA-A2 AntigenmedicineHumansAmino Acid SequenceAlleleHLA-A AntigensMultiple sclerosisMacrophagesMyelin Basic Proteinmedicine.diseaseMyelin basic proteinImmunologybiology.proteinitalian populationCD8PLoS ONE
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Freshly isolated mouse 4F7+ splenic dendritic cells process and present exogenous antigens to T cells.

1994

The antibody 4F7 was reported to recognize an epitope expressed on dendritic cells (DC) from various tissues. To study the ability of splenic 4F7+ dendritic cells to process antigen for presentation to CD4+ T cells, DC were enriched using a separation procedure avoiding overnight culture which could lead to an altered phenotype. These DC were used as antigen-presenting cells (APC) in stimulation cultures of major histocompatibility complex class II-restricted T cells. It was found that they induce antigen-dependent lymphokine production by T cells and therefore could present exogenous antigens. These processing takes place intracellularly, because fixation abrogates presentation to T cells.…

MaleTime FactorsOvalbuminT cellT-LymphocytesImmunologyAntigen presentationAntigen-Presenting CellsCell SeparationIn Vitro TechniquesMicemedicineImmunology and AllergyCytotoxic T cellAnimalsAntigen-presenting cellCells CulturedMice Inbred BALB CCD40biologyAntigen processingHistocompatibility Antigens Class IIAntibodies MonoclonalDendritic cellDendritic CellsNatural killer T cellMolecular biologyCell biologymedicine.anatomical_structureAntigens Surfacebiology.proteinFemalePeptidesSpleenEuropean journal of immunology
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Processing and MHC class I presentation of human cytomegalovirus pp65-derived peptides persist despite gpUS2–11-mediated immune evasion

2007

Immune control of human cytomegalovirus (HCMV) infection can be mediated by CD8+cytolytic T lymphocytes (CTL). Adoptive transfer of antiviral CTL confers protection against HCMV reactivation and disease. The tegument protein pp65 and the immediate-early 1 protein (IE1) are recognized to be major CTL targets, even though during productive infection the viral immunoevasion proteins gpUS2–11 act to suppress major histocompatibility complex (MHC) class I-restricted antigen presentation. Thus it was not clear how infected cells could be labelled with antigenic peptides in the face of immunoevasion. We show here that the immunodominant peptide pp65NLVwas presented by MHC class I in cells infected…

MalevirusesForeskinAntigen presentationCytomegalovirusMice TransgenicBiologyMajor histocompatibility complexCell LineViral Matrix ProteinsMiceImmune systemVirologyHLA-A2 AntigenMHC class IAnimalsHumansAntigen processingHistocompatibility Antigens Class Ivirus diseasesMHC restrictionPhosphoproteinsVirologyPeptide FragmentsCTL*Gene Expression RegulationCytomegalovirus InfectionsImmunologybiology.proteinCD8T-Lymphocytes CytotoxicJournal of General Virology
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Functional cysteine-less subunits of the transporter associated with antigen processing (TAP1 and TAP2) by de novo gene assembly

2002

AbstractWithin the adaptive immune system the transporter associated with antigen processing (TAP) plays a pivotal role in loading of peptides onto major histocompatibility (MHC) class I molecules. As a central tool to investigate the structure and function of the TAP complex, we created cysteine-less human TAP subunits by de novo gene synthesis, replacing all 19 cysteines in TAP1 and TAP2. After expression in TAP-deficient human fibroblasts, cysteine-less TAP1 and TAP2 are functional with respect to adenosine triphosphate (ATP)-dependent peptide transport and inhibition by ICP47 from herpes simplex virus. Cysteine-less TAP1 and TAP2 restore maturation and intracellular trafficking of MHC c…

Models MolecularBiophysicsBiological Transport ActiveBiologyMajor histocompatibility complexTransfectionBiochemistryCell Linechemistry.chemical_compoundAdenosine TriphosphateStructural BiologyATP Binding Cassette Transporter Subfamily B Member 3Cysteine-scanning mutagenesisMHC class IGeneticsHumansCysteineATP Binding Cassette Transporter Subfamily B Member 2Molecular BiologyAntigen PresentationAntigen processingHistocompatibility Antigens Class ICell BiologyTransporter associated with antigen processingMolecular biologyRecombinant ProteinsCell biologyProtein SubunitschemistryAmino Acid SubstitutionAntigen processingPeptide transportMembrane proteinbiology.proteinAdenosine triphosphate-binding cassette transporterTAP2ATP-Binding Cassette TransportersTAP1Adenosine triphosphateFEBS Letters
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Antigen processing influences HIV-specific cytotoxic T lymphocyte immunodominance

2016

Udgivelsesdato: 2009-May-03 Although cytotoxic T lymphocytes (CTLs) in people infected with human immunodeficiency virus type 1 can potentially target multiple virus epitopes, the same few are recognized repeatedly. We show here that CTL immunodominance in regions of the human immunodeficiency virus type 1 group-associated antigen proteins p17 and p24 correlated with epitope abundance, which was strongly influenced by proteasomal digestion profiles, affinity for the transporter protein TAP, and trimming mediated by the endoplasmatic reticulum aminopeptidase ERAAP, and was moderately influenced by HLA affinity. Structural and functional analyses demonstrated that proteasomal cleavage 'prefer…

Models MolecularProteasome Endopeptidase ComplexHIV AntigensMolecular Sequence DataImmunologyAntigen presentationHIV Core Protein p24HIV InfectionsImmunodominanceMajor histocompatibility complexgag Gene Products Human Immunodeficiency VirusEpitopeEvolution MolecularMajor Histocompatibility ComplexLeucyl Aminopeptidase03 medical and health sciences0302 clinical medicineAntigenHumansImmunology and AllergyCytotoxic T cellAmino Acid Sequence030304 developmental biologyAntigen Presentation0303 health sciencesHLA-A AntigensbiologyImmunodominant EpitopesAntigen processingVirology3. Good healthCTL*MutationHIV-1biology.proteinATP-Binding Cassette TransportersProtein BindingT-Lymphocytes Cytotoxic030215 immunologyRETROVIROLOGY
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Control of murine cytomegalovirus in the lungs: Relative but not absolute immunodominance of the immediate-early 1 nonapeptide during the antiviral c…

1998

Effective control by the immune system is a hallmark of cytomegalovirus (CMV) infection. Accordingly, human CMV disease is a medical problem restricted to the immunologically immature or immunocompromised host (for a review, see reference 21). Murine models have implicated natural killer (NK) cells and CD8 T cells in the control of CMV infection. While NK cells mediate early protection in genetically resistant mouse inbred strains (4, 5, 31, 51), CD8 T cells establish enduring protective memory and function as principal antiviral effectors in susceptible strains (31). Specifically, in the BALB/c strain, major histocompatibility complex (MHC) class I-restricted antiviral CD8 T cells resolve …

MuromegalovirusAdoptive cell transferImmunologyViral Pathogenesis and ImmunityBone Marrow CellsImmunodominanceVirus ReplicationMajor histocompatibility complexMicrobiologyImmediate-Early ProteinsMiceImmune systemAntigenVirologyMHC class IAnimalsCytotoxic T cellLungAntigen PresentationMice Inbred BALB CbiologyImmunodominant EpitopesAntigen processingvirus diseasesHerpesviridae InfectionsVirologyKineticsInsect ScienceImmunologyTrans-Activatorsbiology.proteinFemaleT-Lymphocytes Cytotoxic
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Processing and Presentation of Murine Cytomegalovirus pORFm164-Derived Peptide in Fibroblasts in the Face of All Viral Immunosubversive Early Gene Fu…

2002

ABSTRACTCD8 T cells are the principal effector cells in the resolution of acute murine cytomegalovirus (mCMV) infection in host organs. This undoubted antiviral and protective in vivo function of CD8 T cells appeared to be inconsistent with immunosubversive strategies of the virus effected by early (E)-phase genesm04,m06, andm152. The so-called immune evasion proteins gp34, gp48, and gp37/40, respectively, were found to interfere with peptide presentation at different steps in the major histocompatibility complex (MHC) class I pathway of antigen processing and presentation in fibroblasts. Accordingly, they were proposed to prevent recognition and lysis of infected fibroblasts by cytolytic T…

MuromegalovirusImmunologyAntigen presentationMajor histocompatibility complexMicrobiologyImmediate-Early ProteinsMiceOpen Reading FramesViral ProteinsImmune systemAntigenVirologyMHC class IAnimalsCytotoxic T cellAntigens ViralGenes Immediate-EarlyCells CulturedAntigen PresentationMice Inbred BALB CMembrane GlycoproteinsbiologyAntigen processingFibroblastsVirologyPeptide FragmentsCTL*Insect Sciencebiology.proteinPathogenesis and ImmunityFemaleT-Lymphocytes CytotoxicJournal of Virology
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Cytomegalovirus Encodes a Positive Regulator of Antigen Presentation

2006

ABSTRACT Murine cytomegalovirus encodes three regulators of antigen presentation to antiviral CD8 T cells. According to current paradigms, all three regulators are committed to the inhibition of the presentation of antigenic peptides. Whereas m152/gp40 catalyzes the retention of peptide-loaded major histocompatibility complex (MHC) class I molecules in a cis -Golgi compartment, m06/gp48 binds stably to class I molecules and directs them into the cellular cargo-sorting pathway of lysosomal degradation. Regulator m04/gp34 also binds stably to class I molecules, but unlike m152 and m06, it does not downmodulate MHC class I cell surface expression. It has entered the literature as a direct inhi…

MuromegalovirusImmunologyAntigen presentationRegulatorCD8-Positive T-LymphocytesVirus ReplicationMajor histocompatibility complexMicrobiologyMiceViral ProteinsMuromegalovirusAntigenVirologyMHC class IAnimalsHumansCytotoxic T cellAntigens ViralCells CulturedGlycoproteinsAntigen PresentationMice Inbred BALB CMembrane GlycoproteinsbiologyAntigen processingHistocompatibility Antigens Class IH-2 AntigensFibroblastsEmbryo Mammalianbiology.organism_classificationAdoptive TransferMolecular biologyMice Inbred C57BLInsect ScienceCytomegalovirus Infectionsbiology.proteinPathogenesis and ImmunityFemaleCarrier ProteinsPeptidesT-Lymphocytes CytotoxicJournal of Virology
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