Search results for "Arid"

New Method of DNA Isolation from Two Food Additives Suitable for Authentication in Polymerase Chain Reaction Assays

2005

Locust bean gum and guar gum are galactomannans used as additives (E 410 and E 412, respectively) in the food industry as stabilizing agents. Analytical discrimination between the two additives in gums and foods is now feasible by molecular techniques. However, only complex and time-consuming DNA isolation protocols are available to date. We have developed simple improved protocols to obtain enough DNA suitable for PCR amplification from a few milligrams of commercial E 410 and E 412 additives (containing more than 75% polysaccharides). The suspension of additives in water or 10 mM Tris-HCl, pH 8.5, efficiently recovers DNA suitable for authentication in PCR assays. However, the Tris method…

Structural behaviour differences in low methoxy pectin solutions in the presence of divalent cations (Ca 2+ and Zn 2+ ): a process driven by the bind…

2014

International audience; In this paper, we compare the interactions between low methoxy pectin (LMP) and either Ca2+ or Zn2+ in semi-dilute solutions. Intrinsic viscosity and turbidity measurements reveal that pectin-calcium solutions are more viscous, but yet less turbid, than pectin-zinc ones. To get a molecular understanding on the origin of this rather unexpected behavior, we further performed isothermal titration calorimetry, small angle neutron scattering experiments, as well as molecular dynamics simulations. Our results suggest that calcium cations induce the formation of a more homogeneous network of pectin than zinc cations do. The molecular dynamics simulations indicate that this …

Retention/release equilibrium of aroma compounds in fat-free dairy gels

2010

BACKGROUND: The replacement of fat by thickeners in fat-free yoghurts leads to an important modification of aroma compound partitioning, with an impact on aroma perception. Investigation of retention/release equilibria allows a good understanding of aroma compound behaviour depending on food composition. RESULTS: Vapour/liquid equilibria of ten aroma compounds (two esters, two ketones, three aldehydes and three alcohols) in several media were studied to investigate the influence of pectin addition to fat-free dairy gel on the retention/release equilibrium. The partition coefficient of each aroma compound was measured by headspace analysis at equilibrium in six media (pure water, low-methoxy…

Biomaterial-Assisted Anastomotic Healing: Serosal Adhesion of Pectin Films

2021

Anastomotic leakage is a frequent complication of intestinal surgery and a major source of surgical morbidity. The timing of anastomotic failures suggests that leaks are the result of inadequate mechanical support during the vulnerable phase of wound healing. To identify a biomaterial with physical and mechanical properties appropriate for assisted anastomotic healing, we studied the adhesive properties of the plant-derived structural heteropolysaccharide called pectin. Specifically, we examined high methoxyl citrus pectin films at water contents between 17–24% for their adhesivity to ex vivo porcine small bowel serosa. In assays of tensile adhesion strength, pectin demonstrated significant…

Transfers of small analytes in a multiphasic stirred fruit yoghurt model

2007

International audience; The transfer of small analytes in a multiphasic stirred fruit yoghurt model, made of a pectin gel aimed to mimic fruit pieces and of a dairy gel done with milk acidified by glucono-δ-lactone hydrolysis, have been studied. The concentration gradients between the pectin gel and the dairy gel were the driving force for the migration of small analytes (i.e. water, protons and colorants). Water migrated from the dairy to the pectin gel, causing modifications in the water content of both gels and an equilibration of their water activity at 0.938±0.003. Inversely, protons migrated from the pectin to the dairy gel. These changes in composition of both gels being likely to ha…

Spent Coffee Grounds Extract, Rich in Mannooligosaccharides, Promotes a Healthier Gut Microbial Community in a Dose-Dependent Manner.

2019

Coffee is one of the most consumed beverages around the world, and as a consequence, spent coffee grounds are a massively produced residue that is causing environmental problems. Reusing them is a major focus of interest presently. We extracted mannooligosaccharides (MOS) from spent coffee grounds and submitted them to an in vitro fermentation with human feces. Results obtained suggest that MOS are able to exert a prebiotic effect on gut microbiota by stimulating the growth of some beneficial genera, such as Barnesiella, Odoribacter, Coprococcus, Butyricicoccus, Intestinimonas, Pseudoflavonifractor, and Veillonella. Moreover, short-chain fatty acids (SCFA) production also increased in a dos…

El aprendizaje servicio: una experiencia de participación y compromiso

2011

El Aprendizaje Servicio es una metodología innovadora que integra el servicio a la comunidad con el aprendizaje de contenidos, habilidades y valores. Desde el aprendizaje servicio se vincula la enseñanza a la vida misma, abriendo las puertas de la escuela a la comunidad. Se trata de aprender ayudando y de ayudar mientras se aprende. Este aprendizaje puesto en práctica tiene unos fines solidarios que a su vez mejoran la calidad de vida de la comunidad. Service Learning is an innovative methodology that integrates service to the community with the learning of content, skills and values. From service learning, teaching is linked to life itself, opening the doors of the school to the community.…

Gamma irradiation of xyloglucans and hydroxypropyl cellulose in dry and humid solid state to tune molecular weight distribution

2022

Soil Salinity Modeling, Approaches, and Key Issues

2012

Salinization is a progressive soil and water degradation process. Soil salinity can be natural or induced by human affecting aquifers and the most productive irrigated agroecosystems in arid and semiarid regions, representing an increasing environmental concern. Root zone soil salinity can be managed using advanced tools and adjusting irrigation application and using the concept of leaching requirement. Modeling the reactive transport in soil uses simplified representations of the reality, but can reveal complex interrelations of properties of the system under study, and is best suited for drawing scenarios for investigating “what-if…” questions. Each modeling effort tries to give answer to…

A multidisciplinary approach to reveal the Sicily Climate and Environment over the last 20 000 years

2010

Abstract We present a thorough review of the knowledge on the climate and environment in Sicily over the last 20 000 years, taking into account results of several studies carried using terrestrial and marine records. We obtain a coherent framework of the most important changes succeeded in the island, even if some points need further investigation. All the reconstructions of surface temperatures of the seas and the air surrounding Sicily point out severe climatic conditions during the last glacial period. The steppe- and semisteppe-like vegetation pattern testifies, together with additional evidence from geochemical data of lacustrine evidence, markedly arid conditions. Fi-nally, significan…