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showing 10 items of 953 documents

Translating intracarotid artery transplantation of bone marrow-derived NCS-01 cells for ischemic stroke: Behavioral and histological readouts and mec…

2019

Abstract The present study used in vitro and in vivo stroke models to demonstrate the safety, efficacy, and mechanism of action of adult human bone marrow‐derived NCS‐01 cells. Coculture with NCS‐01 cells protected primary rat cortical cells or human neural progenitor cells from oxygen glucose deprivation. Adult rats that were subjected to middle cerebral artery occlusion, transiently or permanently, and subsequently received intracarotid artery or intravenous transplants of NCS‐01 cells displayed dose‐dependent improvements in motor and neurological behaviors, and reductions in infarct area and peri‐infarct cell loss, much better than intravenous administration. The optimal dose was 7.5 × …

Male0301 basic medicinecell lofunctional recoverymedicine.medical_treatmentBasic fibroblast growth factorCell- and Tissue-Based TherapyPharmacologycerebral ischemia03 medical and health scienceschemistry.chemical_compound0302 clinical medicineBone Marrowmental disordersmedicinecytokineAnimalsHumansinfarctcell losslcsh:QH573-671cell transplantationStrokeIschemic Strokelcsh:R5-920business.industrylcsh:CytologyMesenchymal stem cellCell BiologyGeneral MedicineStem-cell therapymedicine.diseaseNeural stem cellcytokinesRatsTransplantation030104 developmental biologymedicine.anatomical_structurechemistrymotor deficitsEnabling Technologies for Cell‐based Clinical TranslationBone marrowStem cellbusinesslcsh:Medicine (General)030217 neurology & neurosurgeryStem Cell TransplantationDevelopmental Biology
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Evaluation of low dose anaphylatoxic peptides in the pathogenesis of the adult respiratory distress syndrome (ARDS). Monitoring of early C5a effects …

1986

A guinea-pig in vivo model is presented that allows the infusion of purified C5a via a central vein catheter and the monitoring of its effects on granulocytes and platelets, the most important cells in the pathogenesis of several lung disorders, e.g. shock lung. After the infusion of C5a, which was adjusted to a quantity that caused slight and transient alterations of lung physiology, granulocytes disappeared from circulation within 1 min. Simultaneously the granulocyte content of the lung increased about three-fold as judged by histological evaluations. Morphologic destructions were not observed. After the drop a rebound of circulating Polymorpho-nuclear leucocytes (PMN) occurred, which wa…

MaleARDSPathologymedicine.medical_specialtyGuinea PigsClinical BiochemistryComplement C5aGranulocyteBiochemistryPathogenesisLung DisorderLeukocyte CountIn vivomedicineAnimalsInfusions IntravenousLungRespiratory Distress SyndromeLungRespiratory distressPlatelet Countbusiness.industryComplement C5General Medicinerespiratory systemmedicine.diseaseDisease Models Animalmedicine.anatomical_structureFemaleBone marrowbusinessGranulocytesEuropean Journal of Clinical Investigation
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HLA association is different in children and adults with severe acquired aplastic anemia

2007

Background Severe aplastic anemia (SAA) is defined as pancytopenia caused by bone marrow failure. The pathogenesis of SAA is thought to involve autoimmune processes. Increased susceptibility to autoimmunity has been shown to be associated with several different HLA alleles. In SAA, few large studies based on data mainly from adults describe a positive HLA correlation with HLA-DR2 (DRB1*15) and HLA-B14. Procedure This study explored the HLA constitution of 181 children with SAA who were enrolled in the prospective multi-center study SAA94 between January 1994 and January 2002. The control group consisted of 303 healthy individuals of comparable demographic background. Allelic frequencies bet…

MaleAdolescentHuman leukocyte antigenmedicine.disease_causeAutoimmunityPathogenesisHLA-B14 AntigenHLA Antigenshemic and lymphatic diseasesmedicineHumansHLA-DR2 AntigenProspective StudiesChildAllelesbusiness.industryBone marrow failureAnemia AplasticInfantHematologymedicine.diseasePancytopeniaPathophysiologyExact testOncologyHLA-B AntigensChild PreschoolPediatrics Perinatology and Child HealthImmunologyCohortFemalebusinessPediatric Blood & Cancer
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Age-dependent alterations of DNA synthesis. Terminal deoxynucleotidyl transferase and DNA polymerase activities in bone marrow subpopulations from mi…

1980

Abstract The decrease of functional capacity of cellular immunity during ageing seems to be due to cellular changes of stem cells, particularly in the growth properties and the cell density in T-cell subsets. We approached this problem at the molecular biological level by quantifying the key enzymes necessary for DNA synthesis in bone marrow cells from mice: deoxynucleotidyl transferase (TdT) and DNA polymerase α. The bone marrow cells were fractionated on a discontinuous bovine serum albumin density gradient and the extractable enzyme activities (expressed per 10 8 nucleated cells in the respective fraction) were determined. TdT activity was found to decrease markedly during ageing. Mature…

MaleAgingCellular immunitybiologyDNA synthesisDNA polymeraseBone Marrow CellsDNA Polymerase IIDNA-Directed DNA PolymeraseMolecular biologyMicemedicine.anatomical_structureTerminal deoxynucleotidyl transferaseBone MarrowDNA NucleotidylexotransferaseAgeingDNA Nucleotidyltransferasesbiology.proteinmedicineAnimalsBone marrowBovine serum albuminStem cellDevelopmental BiologyMechanisms of Ageing and Development
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Oscillatory Dynamics Underlying Perceptual Narrowing of Native Phoneme Mapping from 6 to 12 Months of Age

2016

During the first months of life, human infants process phonemic elements from all languages similarly. However, by 12 months of age, as language-specific phonemic maps are established, infants respond preferentially to their native language. This process, known as perceptual narrowing, supports neural representation and thus efficient processing of the distinctive phonemes within the sound environment. Although oscillatory mechanisms underlying processing of native and non-native phonemic contrasts were recently delineated in 6-month-old infants, the maturational trajectory of these mechanisms remained unclear. A group of typically developing infants born into monolingual English families, …

MaleAgingmedicine.medical_specialtysource localizationSpeech perceptionFirst languageperceptual narrowingAudiologyAuditory cortexSemanticsLanguage Development050105 experimental psychologyDevelopmental psychology03 medical and health sciencesvärähtelyt0302 clinical medicineBiological ClocksGamma RhythmmedicinePerceptual narrowingGamma RhythmHumans0501 psychology and cognitive sciencesTheta RhythmResearch ArticlesLanguageAuditory Cortextime-frequency analysesinfantsGeneral Neuroscience05 social sciencesInfantSemanticsphonemic mappingLanguage developmentEvoked Potentials AuditorySpeech PerceptionFemaleSyllablePsychology030217 neurology & neurosurgeryThe Journal of Neuroscience
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In activated mast cells, IL-1 up-regulates the production of several Th2-related cytokines including IL-9.

2000

Abstract Mast cells can play detrimental roles in the pathophysiology and mortality observed in anaphylaxis and other Th2-dominated allergic diseases. In contrast, these cells contribute to protective host defense mechanisms against parasitic worm infections. After IgE/Ag activation, mast cells can produce multiple cytokines that may enhance allergic inflammations, while a similar panel of Th2-related cytokines may support immunological strategies against parasites. Here we report that in primary mouse bone marrow-derived mast cells activated by ionomycin or IgE/Ag, the proinflammatory mediator IL-1 (α or β) up-regulated production of IL-3, IL-5, IL-6, and IL-9 as well as TNF, i.e., cytokin…

MaleAllergymedicine.medical_treatmentImmunologyDose-Response Relationship ImmunologicInflammationBone Marrow CellsBiologyImmunoglobulin EProinflammatory cytokineImmunophenotypingchemistry.chemical_compoundMiceTh2 CellsAdjuvants ImmunologicmedicineImmunology and AllergyAnimalsMast CellsRNA MessengerMice Inbred BALB CIonomycinInterleukin-9Cell DifferentiationSerum Albumin BovineImmunoglobulin Emedicine.diseaseUp-RegulationInterleukin 33Autocrine CommunicationKineticsCytokinechemistryIonomycinImmunologybiology.proteinCytokinesTumor necrosis factor alphaFemaleInterleukin-4medicine.symptomDinitrophenolsInterleukin-1Journal of immunology (Baltimore, Md. : 1950)
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Allogeneic Stem Cell Transplantation for Myelodysplastic Syndrome Patients with a 5q Deletion

2018

The deletion (5q) karyotype (del [5q]) in patients with myelodysplastic syndrome (MDS) is the most common karyotypic abnormality in de novo MDS. An increased number of blasts and additional karyotypic abnormalities (del [5q]+) are associated with a poor outcome. We analyzed the outcome of allogeneic hematopoietic cell transplants (HCT) in patients suffering from MDS with only del (5q) or del (5q)+ . A total of 162 patients, of median age 54 years (range, 9 to 73), having MDS and del (5q) abnormalities received HCT from identical siblings (n = 87) or unrelated donors (n = 75). The cumulative incidence of nonrelapse mortality and relapse incidence at 4 years was 29% (95% CI, 22 to 36) and 46%…

MaleBLOODDatabases FactualIMPACTCHROMOSOMECancer development and immune defence Radboud Institute for Molecular Life Sciences [Radboudumc 2]medicine.medical_treatment[SDV]Life Sciences [q-bio]MedizinHematopoietic stem cell transplantationPROGNOSTIC SCORING SYSTEMGastroenterology0302 clinical medicineRecurrencehemic and lymphatic diseasesMDSCumulative incidenceLENALIDOMIDEIncidenceIncidence (epidemiology)Hazard ratioHematopoietic Stem Cell TransplantationHematologyMiddle AgedAllograftsTP53 MUTATIONSEUROPEAN-SOCIETY3. Good healthSurvival Rate030220 oncology & carcinogenesisWORKING PARTYChromosomes Human Pair 5FemaleChromosome DeletionLife Sciences & BiomedicineDEL(5Q)del (5q)medicine.drugAdultmedicine.medical_specialtyImmunology3122 CancersDisease-Free SurvivalSettore MED/01 - Statistica Medica03 medical and health sciencesSex FactorsAll institutes and research themes of the Radboud University Medical CenterInternal medicinemedicineHumansMARROW-TRANSPLANTATIONSurvival rateLenalidomideTransplantationScience & Technologybusiness.industryMyelodysplastic syndromesmedicine.diseaseAllogeneic stem cell transplantationTransplantationMyelodysplastic Syndromesbusiness030215 immunology
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Physiological stress does not increase with urbanization in European blackbirds: Evidence from hormonal, immunological and cellular indicators

2020

Urbanization changes the landscape structure and ecological processes of natural habitats. While urban areas expose animal communities to novel challenges, they may also provide more stable environments in which environmental fluctuations are buffered. Species´ ecology and physiology may determine their capacity to cope with the city life. However, the physiological mechanisms underlying organismal responses to urbanization, and whether different physiological systems are equally affected by urban environments remain poorly understood. This severely limits our capacity to predict the impact of anthropogenic habitats on wild populations. In this study, we measured indicators of physiological…

MaleBLOOD-CELLSEnvironmental Engineering010504 meteorology & atmospheric sciencesEcology (disciplines)URBAN010501 environmental sciencesCellular levelBiologyStress01 natural sciencesSongbirdsBirdsPASSER-DOMESTICUSStress PhysiologicalUrbanizationBODY CONDITION11. SustainabilityAnimalsEnvironmental ChemistryCitiesWaste Management and DisposalEcosystemFEATHER CORTICOSTERONEPhysiological stress0105 earth and related environmental sciencesEcologyLandscape structureTURDUS-MERULAUrbanization15. Life on landPollutionPhysiological responses3. Good healthHabitatH/L ratioHOUSE SPARROWSLEUKOCYTE PROFILESHeat-shock proteins[SDE.BE]Environmental Sciences/Biodiversity and EcologyCorticosteroneFOOD SUPPLEMENTATIONHormoneScience of The Total Environment
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Rituximab with cyclophosphamide, vincristine, non-pegylated liposomal doxorubicin and prednisone as first-line treatment for splenic marginal zone ly…

2015

Rituximab ® provides high response rates and effective disease palliation in patients with splenic marginal zone lymphoma (SMZL). We conducted a phase II trial in patients with SMZL who were either untreated or were splenectomized but had shown disease progression within 1 year after splenectomy. Treatment consisted of six courses of Rituximab with cyclophosphamide, vincristine, non-pegylated liposomal doxorubicin and prednisone (R-COMP). Fifty-one patients were eligible for the analysis. The overall response rate was 84%. The 6-year progression-free survival and overall survival were 54% and 72%, respectively. Toxicity was substantial (grade ≥ 3 neutropenia: 26%; grade ≥ 3 infections: 8%).…

MaleCancer ResearchBiopsymedicine.medical_treatmentfirst lineKaplan-Meier EstimateSplenic marginal zone lymphoma; first line; rituximabPolyethylene GlycolGastroenterologyPolyethylene GlycolsrituximabBone MarrowPrednisonefirst line; rituximab; splenic marginal zone lymphomaCause of DeathAntineoplastic Combined Chemotherapy ProtocolsSplenic marginal zone lymphomaAged 80 and overHematologyMiddle AgedPrognosisCombined Modality TherapySplenic NeoplasmTreatment OutcomeItalyOncologyVincristineFemaleRituximabHumanmedicine.drugAdultmedicine.medical_specialtyVincristineLymphoma B-CellCyclophosphamidePrognosiSplenectomySplenic NeoplasmNeutropeniaImmunophenotypingfirst line; rituximab; Splenic marginal zone lymphoma; Adult; Aged; Aged 80 and over; Antineoplastic Combined Chemotherapy Protocols; Biomarkers; Biopsy; Bone Marrow; Cause of Death; Combined Modality Therapy; Cyclophosphamide; Doxorubicin; Female; Humans; Immunophenotyping; Italy; Kaplan-Meier Estimate; Lymphoma B-Cell; Male; Middle Aged; Polyethylene Glycols; Prednisone; Prognosis; Rituximab; Splenic Neoplasms; Treatment Outcome; Vincristine; Hematology; Oncology; Cancer ResearchInternal medicinemedicineHumansSplenic marginal zone lymphomaCyclophosphamideAgedAntineoplastic Combined Chemotherapy Protocolbusiness.industrySplenic NeoplasmsBiomarkermedicine.diseaseSurgeryDoxorubicinPrednisonebusinessBiomarkers
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Exosome-mediated crosstalk between chronic myelogenous leukemia cells and human bone marrow stromal cells triggers an Interleukin 8-dependent surviva…

2014

Chronic myelogenous leukemia (CML) is a myeloproliferative disorder characterized by the Bcr-Abl oncoprotein with constitutive tyrosine kinase activity. Exosomes are nanovesicles released by cancer cells that are involved in cell-to-cell communication thus potentially affecting cancer progression. It is well known that bone marrow stromal microenvironment contributes to disease progression through the establishment of a bi-directional crosstalk with cancer cells. Our hypothesis is that exosomes could have a functional role in this crosstalk. Interleukin-8 (IL 8) is a proinflammatory chemokine that activates multiple signalling pathways downstream of two receptors (CXCR1 and CXCR2). We demon…

MaleCancer ResearchChemokineStromal cellCell SurvivalMice SCIDExosomesChronic myelogenous leukemia Bone marrow stromal cells Tumour microenvironment Exosomes Interleukin 8ExosomeMiceCell MovementMice Inbred NODSettore BIO/13 - Biologia ApplicataCell Line TumorLeukemia Myelogenous Chronic BCR-ABL Positivehemic and lymphatic diseasesParacrine CommunicationCell AdhesionTumor MicroenvironmentmedicineAnimalsHumansCXC chemokine receptorsStem Cell NichebiologyInterleukin-8Mesenchymal Stem Cellsmedicine.diseaseUp-RegulationLeukemiaPhenotypemedicine.anatomical_structureOncologyCancer cellImmunologyCancer researchbiology.proteinHeterograftsBone marrowSignal TransductionChronic myelogenous leukemiaCancer Letters
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