Search results for "Asparagine"

Synthesis of C1-[18F]fluoroethylamnino asparagine for imaging cancer

2001

C1-[18F]fluoroethylamino asparagine was synthesised from N1-t-boc C1-p-nitrophenol asparagine and the radiolabelled precursor [18F]flouroethylamine in a one-pot-synthesis. The yield of this synthesis was 22% referring to [18F]fluoride, including the removal of the protection groups. The in vivo tests are in progress.

N-glycosylation efficiency is determined by the distance to the C-terminus and the amino acid preceding an Asn-Ser-Thr sequon

2010

N-glycosylation is the most common and versatile protein modification. In eukaryotic cells, this modification is catalyzed cotranslationally by the enzyme oligosaccharyltransferase, which targets the β-amide of the asparagine in an Asn-Xaa-Ser/Thr consensus sequon (where Xaa is any amino acid but proline) in nascent proteins as they enter the endoplasmic reticulum. Because modification of the glycosylation acceptor site on membrane proteins occurs in a compartment-specific manner, the presence of glycosylation is used to indicate membrane protein topology. Moreover, glycosylation sites can be added to gain topological information. In this study, we explored the determinants of N-glycosylati…

Studies on the Content of Free Amino Acids in Needles of Undamaged and Damaged Spruce Trees at a Natural Habitat

1990

Summary Measurements of the amino acids in needles of undamaged and damaged 90-year-old spruce trees ( Picea abies ) showed different patterns. During the summer months a reduction in total amino acid content of about 60 % was found. In autumn the content of total amino acids rose again. The courses of the single amino acids behave similarly to that of the total amino acids. Quantitatively, the main amino acids were GLU, ASP and GLN with about 37 %, 21 % and 10 %, respectively, followed by PRO, ALA and SER with about 3–4 % each. In the needles of healthy trees large variations in ARG content could be observed. These variations correlate neither with the damage degree of the trees nor with t…

Reducing of acrylamide formation in wheat biscuits supplemented with flaxseed and lupine

2016

Abstract The use of pseudo-cereals for wheat products making is to fortify the deficiency of nutritional value in wheat flour. However rich in proteins plant additives could increase acrylamide content in baked products. The present study was focused on acrylamide reduction in wheat flour biscuits supplemented with lupine and defatted flaxseed flour treated by solid state (SSF) and submerged (SMF) fermentations by Lactobacillus sakei , Pediococcus pentosaceus and Pediococcus acidilactici strains. After fermentation the decrease in asparagine was on average of 67.6 and 80.6%, and reducing sugar contents were reduced by 18 and 79.4% in flaxseed and lupine, respectively. The most effective acr…

ChemInform Abstract: A Sialyl-Lewis A-Asparagine Building Block for Glycopeptide-Synthesis.

2000

Developments in the Synthesis of Glycopeptides Containing Glycosyl l-Asparagine, l-Serine, and l-Threonine

1994

Novel Mutations in the Asparagine Synthetase Gene (ASNS) Associated With Microcephaly

2018

Microcephaly is a devastating condition defined by a small head and small brain compared to the age- and sex-matched population. Mutations in a number of different genes causative for microcephaly have been identified, e.g., MCPH1, WDR62, and ASPM. Recently, mutations in the gene encoding the enzyme asparagine synthetase (ASNS) were associated to microcephaly and so far 24 different mutations in ASNS causing microcephaly have been described. In a family with two affected girls, we identified novel compound heterozygous variants in ASNS (c.1165G > C, p.E389Q and c.601delA, p.M201Wfs∗28). The first mutation (E389Q) is a missense mutation resulting in the replacement of a glutamate residue …