Search results for "Aβ42"

showing 3 items of 3 documents

Immune profiling of Alzheimer patients

2011

Abstract Alzheimer's disease (AD) is characterized by extracellular senile plaques in the brain, containing amyloid-β peptide (Aβ). We identify immunological differences between AD patients and age-matched controls greater than those related to age itself. The biggest differences were in the CD4 + rather than the CD8 + T cell compartment resulting in lower proportions of naive cells, more late-differentiated cells and higher percentages of activated CD4 + CD25 + T cells without a Treg phenotype in AD patients. Changes to CD4 + cells might be the result of chronic stimulation by Aβ present in the blood. These findings have implications for diagnosis and understanding the aetiology of the dis…

AdultCD4-Positive T-LymphocytesMaleImmunosenescenceT cellImmunologyStimulationDiseaseCD8-Positive T-LymphocytesBiologyYoung AdultAlzheimer DiseaseExtracellularmedicineHumansImmunology and AllergySenile plaquesAgedAged 80 and overSettore MED/04 - Patologia GeneraleGene Expression ProfilingAβ42Age FactorsT cellCell DifferentiationImmunosenescenceMiddle AgedAlzheimer's diseasePhenotypeCD4 Lymphocyte Countmedicine.anatomical_structureNeurologyImmunologyEtiologyFemaleNeurology (clinical)BiomarkersJournal of Neuroimmunology
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Systemic Immune Responses in Alzheimer's Disease: In Vitro Mononuclear Cell Activation and Cytokine Production

2010

To investigate the systemic signs of immune-inflammatory responses in Alzheimer's disease (AD), in the present study we have analyzed blood lymphocyte subsets and the expression of activation markers on peripheral blood mononuclear cells (PBMCs) from AD patients and age-matched healthy controls (HC) activated in vitro by recombinant amyloid-beta peptide (rAbeta42). Our study of AD lymphocyte subpopulations confirms the already described decrease of the absolute number and percentage of B cells when compared to HC lymphocytes, whereas the other subsets are not significantly different in patients and controls. We report the increased expression of the activation marker CD69 and of the chemoki…

MaleEotaxinCCR2ChemokineTime Factorsmedicine.medical_treatmentPeripheral blood mononuclear cellChemokine receptorImmune systemAlzheimer’s disease chemokine cytokine PBMC rAβ42Alzheimer DiseasemedicineHumansLymphocytesIL-2 receptorCells CulturedAgedSettore MED/04 - Patologia GeneraleAged 80 and overAnalysis of VarianceAmyloid beta-Peptidesbiologybusiness.industryGeneral NeuroscienceGeneral MedicineMiddle AgedFlow CytometryPeptide FragmentsPsychiatry and Mental healthClinical PsychologyCytokineGene Expression RegulationCase-Control StudiesImmunologyLeukocytes Mononuclearbiology.proteinCytokinesFemaleGeriatrics and GerontologybusinessJournal of Alzheimer's Disease
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The deubiquitinase USP11 is a versatile and conserved regulator of autophagy

2021

Autophagy is a major cellular quality control system responsible for the degradation of proteins and organelles in response to stress and damage to maintain homeostasis. Ubiquitination of autophagy-related proteins or regulatory components is important for the precise control of autophagy pathways. Here, we show that the deubiquitinase ubiquitin-specific protease 11 (USP11) restricts autophagy and that KO of USP11 in mammalian cells results in elevated autophagic flux. We also demonstrate that depletion of the USP11 homolog H34C03.2 in Caenorhabditis elegans triggers hyperactivation of autophagy and protects the animals against human amyloid-β peptide 42 aggregation-induced paralysis. USP11…

autophagyhAβ42 human amyloid-β protein 1 to 42Lipid kinase activityPI(3)P phosphatidylinositol-3-phosphatemTORC1BiochemistryCell LineGene Knockout Techniqueschemistry.chemical_compoundubiquitinAnimalsHumansULK1 unc-51-like autophagy activating kinase 1WIPI WD-repeat domain phosphoinositide-interacting proteinPI3KC3-C1Caenorhabditis elegansCaenorhabditis elegans ProteinsmTORC1Molecular BiologyMechanistic target of rapamycinUSP11 ubiquitin-specific protease 11proteostasisAmyloid beta-PeptidesS6K S6 kinasebiologyPhosphatidylinositol 3-phosphateAutophagyDUB deubiquitinaseLFQ label-free quantificationIP immunoprecipitationNHT nonhuman targetingPI3KC3-C1 class III phosphatidylinositol 3-kinase complex ICell BiologyACN acetonitrileAβ amyloid-βNRBF2 nuclear receptor-binding factor 2Peptide FragmentsCell biologydeubiquitinase (DUB)ProteostasischemistryProteotoxicitymTORC1 mechanistic target of rapamycin complex 1biology.proteinAutophagy-Related Protein-1 HomologBSA bovine serum albuminThiolester HydrolasesResearch ArticleJournal of Biological Chemistry
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