Search results for "B19"

Molecular and structural characterization of fluorescent human parvovirus B19 virus-like particles

2005

Although sharing a T = 1 icosahedral symmetry with other members of the Parvoviridae family, it has been suggested that the fivefold channel of the human parvovirus B19 VP2 capsids is closed at its outside end. To investigate the possibility of placing a relatively large protein moiety at this site of B19, fluorescent virus-like particles (fVLPs) of B19 were developed. The enhanced green fluorescent protein (EGFP) was inserted at the N-terminus of the structural protein VP2 and assembly of fVLPs from this fusion protein was obtained. Electron microscopy revealed that these fluorescent protein complexes were very similar in size when compared to wild-type B19 virus. Further, fluorescence cor…

Human parvovirus B19 infection and antiphospholipid antibodies

2007

Erythema infectiosum is the main manifestation of human parvovirus B19 infections. Further B19-related diseases commonly associated with the acute infection are flue-like symptoms, transient aplastic crisis, transient arthralgias, leukopenia and thrombocytopenia, spontaneous abortion and hydrops fetalis in pregnant women. Hepatitis, myocarditis, meningitis, encephalitis as well as pure red cell anemia may occur occasionally. In addition parvovirus B19 infections have been frequently described as cause or trigger of various forms of autoimmune diseases affecting all blood cell lines, joints, connective tissue, uvea, large and small vessels. Molecular mimicry may be one major contribution to …

Parvovirus B19-induced angiogenesis in fulminant myocarditis

2020

Human parvovirus B19 induced apoptotic bodies contain altered self-antigens that are phagocytosed by antigen presenting cells.

2013

Human parvovirus B19 (B19V) from the erythrovirus genus is known to be a pathogenic virus in humans. Prevalence of B19V infection has been reported worldwide in all seasons, with a high incidence in the spring. B19V is responsible for erythema infectiosum (fifth disease) commonly seen in children. Its other clinical presentations include arthralgia, arthritis, transient aplastic crisis, chronic anemia, congenital anemia, and hydrops fetalis. In addition, B19V infection has been reported to trigger autoimmune diseases such as systemic lupus erythematosus and rheumatoid arthritis. However, the mechanisms of B19V participation in autoimmunity are not fully understood. B19V induced chronic dise…

Cilvēka parvovīrusa B19 infekcijas marķieru un aktivīna B klātbūtne pacientiem ar ME/CF

2021

Mialģiskais encefalomielīts jeb hroniskā noguruma sindroms ir augsti heterogēna slimība ar nezināmu etiopatoģenēzi. Aktuāla ir nepieciešamība pēc ME/CFS biomarķieru atklāšanas un pacientu stratifikācijas. Iespējams, ME/CFS iedalījums varētu būt ar infekciju saistīta saslimšana. Tādēļ šajā darbā tika pārbaudīta B19 infekcijas marķieru klātbūtne un aktivīna B, potenciālā biomarķiera, koncentrācija ME/CFS pacientiem. Pētījuma ietvaros noteica B19 specifisko IgG/IgM klases antivielu klātbūtni, aktivīna B koncentrāciju, lietojot ELISA testus, un B19 NS genoma sekvences nPCR pilnu asiņu un no šūnām brīvas asins plazmas DNS paraugos ME/CFS pacientiem un praktiski veseliem asins donoriem (PVAD). Ie…

The Putative Metal Coordination Motif in the Endonuclease Domain of Human Parvovirus B19 NS1 Is Critical for NS1 Induced S Phase Arrest and DNA Damage

2011

The non-structural proteins (NS) of the parvovirus family are highly conserved multi-functional molecules that have been extensively characterized and shown to be integral to viral replication. Along with NTP-dependent helicase activity, these proteins carry within their sequences domains that allow them to bind DNA and act as nucleases in order to resolve the concatameric intermediates developed during viral replication. The parvovirus B19 NS1 protein contains sequence domains highly similar to those previously implicated in the above-described functions of NS proteins from adeno-associated virus (AAV), minute virus of mice (MVM) and other non-human parvoviruses. Previous studies have show…

Parvovīrusu genoma secību noteikšana indivīdiem ar encefalopātiju

2018

Latvijā B19V, HBoV un hPARV4 ir maz pētīti. Citur pasaulē ir atsevišķi ziņojumi, kas saista parvovīrusus B19V, HBoV, hPARV4 ar encefalopātiju, tomēr precīzi mehānismi, kā šie vīrusi iesaistās slimības etioloģijā, nav zināmi. Parvovīrusi pieder pie plašas mazu vīrusu dzimtas Parvoviridae. Tiem ir vienpavediena DNS, genoms ir aptuveni 5-6 kilobāžu garš. Vīrusam nav ārējais apvalks, bet gan vīrusa kapsīds. Šī darba mērķis bija pētīt parvovīrusu B19V, HBoV un hPARV4 iespējamo iesaisti neprecizētas encefalopātijas patoģenēzē. No DNS paraugiem, kas izolēti no 28 pacientu ar neprecizētu encefalopātiju pilnām asinīm un no šūnām brīvas asins plazmas, 6/28 (21,43%) bija B19V pozitīvi, 5/25 (20%) – HB…

Immune tolerance disruption by human parvovirus B19 viral infection mechanisms

2013

Virusinfektioita pidetään kasvavissa määrin suurimpana ympäristöperäisenä syynä, joka vaikuttaa autoimmuunisairauksien kehitykseen ja autoimmuniteetin muodostukseen. Esimerkiksi parvovirus B19 (B19V) on yhdistetty punahukkaan sekä nivelreumaan. On todettu, että B19V:n ei-rakenteellinen proteiini 1 (NS1) aiheuttaa apoptoosia soluissa, jotka eivät tavallisesti ole B19V infektion kohteena. Lisäksi hiljattain on osoitettu, että apoptoosin seurauksena muodostuvat apoptoottiset kappaleet kykenevät esittelemään antigeenejä niitä esittelemään erikoistuneille soluille, kuten makrofageille tai dendriittisoluille. Tämän tutkimuksen tarkoituksena on löytää B19V infektioon liittyviä autoantigeenejä sekä…

Generation of multifunctional murine monoclonal antibodies specifically directed to the VP1unique region protein of human parvovirus B19.

2007

Little is known about the VP1unique region (VP1u), a part of one major capsid protein of human parvovirus B19 (B19), concerning its involvement in viral replication and infection cycle. Showing a phospholipase A2 (PLA2)-like activity, which is discussed to be necessary for viral release from host cell, its precise function remains unclear. The purpose of this study was to generate multifunctional monoclonal antibodies (mabs) for different applications that may be useful in investigating VP1u's relevance. To establish antiVP1u antibodies, spleen cells from Balb/c mice immunized with purified recombinant viral protein were used for generating antibody-producing hybridoma cell lines. Usability…

Parvovirus B19-Associated Microvesicular Eruption

2015

We report on a 3-year-old girl with a microvesicular generalized rash in whom primary infection by parvovirus B19 was demonstrated by seroconversion. To our knowledge, this is the first instance of an eruption arising from parvovirus B19 with this peculiar clinical pattern.