Search results for "B1"

showing 10 items of 1109 documents

Hematinic de?ciencies in patients with recurrent aphthous stomatitis : variations by gender and age

2017

Background The aim of this study was to evaluate the association between hematinic deficiencies and recurrent aphthous stomatitis (RAS). Material and Methods 517 RAS patients and 187 healthy controls were enrolled in the present study. Hematinic deficiencies, including serum ferritin, folic acid, and vitamin B12 deficiencies were assessed for each participant. Gender and age were taken into account and the collected data were statistically analysed. Results Compared with the healthy controls, a significantly higher overall frequency of hematinic deficiencies was found in RAS patients (p<0.001). When gender and age were taken into account, significant differences in hematinic deficiencies we…

AdultMalemedicine.medical_specialtyFolic Acid DeficiencyRecurrent aphthous stomatitisLogistic regressionAge and gender03 medical and health sciencesYoung Adult0302 clinical medicineSex FactorsRecurrenceInternal medicinepolycyclic compoundsMedicineHumansIn patientHematinicVitamin B12Young adultGeneral DentistryStomatitisOral Medicine and Pathologybusiness.industryResearchAge FactorsVitamin B 12 Deficiency030206 dentistryMiddle Agedmedicine.disease:CIENCIAS MÉDICAS [UNESCO]Otorhinolaryngology030220 oncology & carcinogenesisFerritinsUNESCO::CIENCIAS MÉDICASSurgeryFemaleStomatitis Aphthousbusiness
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Evidences of +896 A/G TLR4 Polymorphism as an Indicative of Prevalence of Complications in T2DM Patients

2014

T2DM is today considered as world-wide health problem, with complications responsible of an enhanced mortality and morbidity. Thus, new strategies for its prevention and therapy are necessary. For this reason, the research interest has focused its attention on TLR4 and its polymorphisms, particularly the rs4986790. However, no conclusive findings have been reported until now about the role of this polymorphism in development of T2DM and its complications, even if a recent meta-analysis showed its T2DM association in Caucasians. In this study, we sought to evaluate the weight of rs4986790 polymorphism in the risk of the major T2DM complications, including 367 T2DM patients complicated for th…

AdultMalemedicine.medical_specialtyGenotypeArticle SubjectT2DM TLR4 +896A/G SNP T2DM complicationsImmunologyPolymorphism Single NucleotideLower limbGene FrequencyDiabetes mellitusInternal medicineGenotypelcsh:PathologymedicineHumansSettore MED/05 - Patologia ClinicaGenetic Predisposition to DiseaseAllele frequencyAgedAged 80 and overSettore MED/04 - Patologia Generalebusiness.industryConfoundingTLR4 POLYMORPHISMCell BiologyMiddle Agedmedicine.diseaseSurgeryToll-Like Receptor 4Cumulative riskDiabetes Mellitus Type 2FemaleComplicationbusinessResearch Articlelcsh:RB1-214
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Association of C677T polymorphism in MTHFR gene, high homocysteine and low HDL cholesterol plasma values in heterozygous familial hypercholesterolemi…

2010

Aim: to investigate the association of C677T polymorphism in the methylene tetrahydrofolate reductase (MTHFR) gene, homocysteine plasma values (Hcy), and plasma HDL cholesterol in heterozy-gous familial hypercholesterolemia (hFH).Methods: One hundred and twenty-five hFH subjects were studied. Plasma lipid, lipoprotein, vitamin B12, folic acid and Hcy values were determined. C677T polymorphism in the MTHFR gene was detected by SSCP-PCR. Genetic diagnosis of FH was determined by a three-step protocol using SSCP-PCR, Southern blot, long PCR and automatic sequencing.Results: We found significant differences in plasma HDL-C (CC 1.39±0.34, CT 1.33±0.39 and TT 1.14±0.26 mmol/L, p=0.028) between th…

AdultMalemedicine.medical_specialtyHeterozygoteApolipoprotein BHomocysteineHypercholesterolemiaFamilial hypercholesterolemiaPolymerase Chain Reactionchemistry.chemical_compoundInternal medicineGenotypeInternal MedicinemedicineHumansVitamin B12HomocysteineMethylenetetrahydrofolate Reductase (NADPH2)Polymorphism Single-Stranded ConformationalApolipoproteins BGeneticsPolymorphism GeneticbiologyCholesterolbusiness.industryBiochemistry (medical)Cholesterol HDLMiddle Agedmedicine.diseaseEndocrinologychemistryReceptors LDLMethylenetetrahydrofolate reductaseMutationbiology.proteinFemaleCardiology and Cardiovascular MedicinebusinessLipoproteinJournal of atherosclerosis and thrombosis
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Blood lipids, homocysteine, stress factors, and vitamins in clinically stable multiple sclerosis patients

2009

Abstract Multiple Sclerosis (MS) patients present a decrease of antioxidants and neuroprotective and immunoregulatory vitamins and an increase of total homocysteine (tHcy), cholesterol (CHL), HDL-cholesterol, and of cellular stress markers, variably associated with the different phases of the disease. We compared the blood levels of uric acid, folic acid, vitamins B12, A, and E, tHcy, CHL, HDL-cholesterol, and triglycerides in forty MS patients during a phase of clinical inactivity with those of eighty healthy controls, matched for age and sex. We found higher levels of tHcy (p = 0.032) and of HDL-cholesterol (p = 0.001) and lower levels of vitamin E (p = 0.001) and the ratio vitamin E/CHL …

AdultMalemedicine.medical_specialtyMultiple SclerosisAdolescentHomocysteineEndocrinology Diabetes and Metabolismmedicine.medical_treatmentClinical BiochemistryBlood lipidsClinical nutritionBiologyAntioxidantschemistry.chemical_compoundFolic AcidEndocrinologyHigh-density lipoproteinSettore BIO/10 - BiochimicaInternal medicinemedicineHumansShort PaperVitamin B12Homocysteinelcsh:RC620-627TriglyceridesBiochemistry medicalCholesterolVitamin EBiochemistry (medical)VitaminsMiddle AgedLipidsUric Acidlcsh:Nutritional diseases. Deficiency diseasesEndocrinologychemistryCase-Control StudiesUric acidSettore MED/26 - NeurologiaFemalelipids (amino acids peptides and proteins)Lipids in Health and Disease
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Analysis of thiamine transporter genes in sporadic beriberi

2014

Abstract Objective Thiamine or vitamin B 1 deficiency diminishes thiamine-dependent enzymatic activity, alters mitochondrial function, impairs oxidative metabolism, and causes selective neuronal death. We analyzed for the first time, the role of all known mutations within three specific thiamine carrier genes, SLC19 A2, SLC19 A3 , and SLC25 A19 , in a patient with atrophic beriberi, a multiorgan nutritional disease caused by thiamine deficiency. Methods A 44-year-old male alcoholic patient from Morocco developed massive bilateral leg edema, a subacute sensorimotor neuropathy, and incontinence. Despite normal vitamin B 1 serum levels, his clinical picture was rapidly reverted by high-dose in…

AdultMalemedicine.medical_specialtySLC19 A- SLC25 A19SLC19 AEndocrinology Diabetes and MetabolismGene mutationBeriberimedicine.disease_causeMitochondrial Membrane Transport Proteinslaw.inventionBeriberilawInternal medicineGenotypemedicineThiamine transporterObjective: Thiamine or vitamin B1 deficiency diminishes thiamine-dependent enzymatic activity alters mitochondrial function impairs oxidative metabolism and causes selective neuronal death. We analyzed for the first time the role of all known mutations within three specific thiamine carrier genes SLC19 A2 SLC19 A3 and SLC25 A19 in a patient with atrophic beriberi a multiorgan nutritional disease caused by thiamine deficiency. Methods: A 44-year-old male alcoholic patient from Morocco developed massive bilateral leg edema a subacute sensorimotor neuropathy and incontinence. Despite normal vitamin B1 serum levels his clinical picture was rapidly reverted by high-dose intramuscular thiamine treatment suggesting a possible genetic resistance. We used polymerase chain reaction followed by amplicon sequencing to study all the known thiamine-related gene mutations identified within the Human Gene Mutation Database. Results: Thirty-seven mutations were tested: 29 in SLC19 A2 6 in SLC19 A3 and 2 in SLC25 A19. Mutational analyses showed a wild-type genotype for all sequences investigated. Conclusion: This is the first genetic study in beriberi disease. We did not detect any known mutation in any of the three genes in a sporadic dry beriberi patient. We cannot exclude a role for other known or unknown mutations in the same genes or in other thiamine-associated genes in the occurrence of this nutritional neuropathy.HumansThiamineGenePolymerase chain reactionGeneticsMutationNutrition and DieteticsbiologyMembrane Transport ProteinsThiamine Deficiencymedicine.diseaseAlcoholismEndocrinologyMutationbiology.proteinThiamineMutations
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Left ventricular filling abnormalities and obesity-associated hypertension: relationship with overproduction of circulating transforming growth facto…

2005

This study has been designed to evaluate the relationship among transforming growth factor beta1 (TGFbeta1) and some measurements of diastolic function in a population of hypertensive subjects with normal left ventricular ejection fraction. We studied 67 hypertensive outpatients who according to their BMI levels were subdivided into three groups: lean (L), overweight (OW) and obese (OB) hypertensives (HT). Circulating TGFbeta1 and M- and B-mode echocardiography was determined. All hypertensives were further subgrouped, according to European Society of Cardiology Guidelines, into two subsets of patients with normal diastolic function or with diastolic dysfunction. Prevalence of left ventricu…

AdultMalemedicine.medical_specialtySettore MED/09 - Medicina InternaHeart VentriclesEnzyme-Linked Immunosorbent Assayobesity-associated hypertension; TGFb1; left ventricular hypertrophy; left ventricular diastolic functionLeft ventricular hypertrophyTransforming Growth Factor beta1Ventricular Dysfunction LeftDiastoleRisk FactorsTransforming Growth Factor betaVentricule gaucheInternal medicineInternal MedicinemedicineHumansObesityOverproductionAgedbusiness.industryStroke VolumeNutritional statusMiddle Agedmedicine.diseaseMyocardial ContractionSettore MED/11 - Malattie Dell'Apparato CardiovascolareObesityEndocrinologyEchocardiographyHypertensionAdult Aged Biological Markers/blood Diastole Echocardiography Enzyme-Linked Immunosorbent Assay Female Heart Ventricles/physiopathology Heart Ventricles/ultrasonography Humans Hypertension/blood Hypertension/complications* Hypertension/physiopathology Hypertrophy Left Ventricular/blood Hypertrophy Left Ventricular/complications* Hypertrophy Left Ventricular/physiopathology Male Middle Aged Myocardial Contraction/physiology* Obesity/blood Obesity/complications* Obesity/physiopathology Regression Analysis Risk FactorsCardiologyRegression AnalysisFemaleHypertrophy Left VentricularbusinessVentricular fillingBiomarkersTransforming growth factorJournal of Human Hypertension
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Effects of moderate Sicilian red wine consumption on inflammatory biomarkers of atherosclerosis.

2006

Objective: The aim of the study is to evaluate the effect of moderate Sicilian red wine consumption on cardiovascular risk factors and, in particular, on some inflammatory biomarkers. Methods: A total of 48 subjects of both sexes who were nondrinkers or rare drinkers of moderate red wine were selected and randomly subdivided into two groups assigned to receive with a crossover design a Sicilian red wine (Nero d’Avola or Etna Torrepalino) during meals: Group A (n ¼ 24), in whom the diet was supplemented for 4 weeks with 250 ml/day of red wine, followed by 4 weeks when they returned to their usual wine intake; and Group B (n ¼ 24), in whom the usual wine intake was maintained for 4 weeks, fol…

AdultMalemedicine.medical_specialtySettore MED/09 - Medicina InternaMediterranean dietMedicine (miscellaneous)WineClinical nutritionFibrinogenDiet Mediterraneanchemistry.chemical_compoundRisk FactorsMediterranean dietInternal medicinemedicineHumansRisk factorAgedWineNutrition and DieteticsCross-Over StudiesFactor VIIbusiness.industryVascular diseasefood and beveragesred wineMiddle Agedmedicine.diseaseAtherosclerosisLipid MetabolismSettore MED/11 - Malattie Dell'Apparato CardiovascolareCrossover studySurgeryhs-CRPEndocrinologyC-Reactive ProteinCholesterolrisk factorchemistryItalyTGFb1red wine; risk factors; TGFb1; hs-CRP; Mediterranean dietFemaleInflammation MediatorsbusinessOxidation-ReductionBiomarkersmedicine.drugEuropean journal of clinical nutrition
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Increased seroprevalence of parvovirus B 19 IgG in complex regional pain syndrome is not associated with antiendothelial autoimmunity

2005

The etiology of complex regional pain syndrome (CRPS) is unclear yet. Recently autoantibodies and antecedent viral infections have been discussed to be involved in the pathogenesis of CRPS. We investigated sera from 39 CRPS patients and healthy controls for parvovirus B19 IgG and the occurrence of antiendothelial autoantibodies (AECA). CRPS patients showed a higher seroprevalence of parvovirus B19 IgG than controls (p < 0.01). All CRPS 2 patients were positive. 10.2% of the CRPS patients and 10.0% of the controls had AECA (n.s.) and AECA were not associated with parvovirus B19 seropositivity. Our findings suggest the involvement of parvovirus B19, but not autoantibody-mediated endothelial c…

AdultMalevirusesEnzyme-Linked Immunosorbent AssayAntibodies Viralmedicine.disease_causeAutoimmune DiseasesAutoimmunityParvoviridae InfectionsPathogenesisSeroepidemiologic StudiesParvovirus B19 HumanmedicineHumansSeroprevalenceEndotheliumAgedAutoantibodiesbiologybusiness.industryParvovirusAutoantibodyvirus diseasesMiddle Agedbiology.organism_classificationmedicine.diseaseAnesthesiology and Pain MedicineComplex regional pain syndromeImmunoglobulin GImmunologybiology.proteinEtiologyFemaleAntibodybusinessComplex Regional Pain SyndromesEuropean Journal of Pain
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T helper cell-mediated interferon-gamma expression after human parvovirus B19 infection: persisting VP2-specific and transient VP1u-specific activity.

2005

SummaryHuman parvovirus B19 is a small non-enveloped DNA virus with an icosahedral capsid consisting of proteins of only two species, the major protein VP2 and the minor protein VP1. VP2 is contained within VP1, which has an additional unique portion (VP1u) of 227 amino acids. We determined the ability of eukaryotically expressed parvovirus B19 virus-like particles consisting of VP1 and VP2 in the ratio recommended for vaccine use, or of VP2 alone, to stimulate, in an HLA class II restricted manner, peripheral blood mononuclear cells (PBMC) to proliferate and to secrete interferon gamma (IFN-γ) and interleukin (IL)-10 cytokines among recently and remotely B19 infected subjects. PBMC reactiv…

AdultMalevirusesImmunologyPeripheral blood mononuclear cellVirusParvoviridae Infections03 medical and health sciencesEpitopesInterferon-gammaAntigenPregnancymedicineParvovirus B19 HumanImmunology and AllergyHumansInterferon gammaPregnancy Complications InfectiousCells Cultured030304 developmental biologyParvoviridae0303 health sciencesImmunity Cellularbiology030306 microbiologyParvovirusHistocompatibility Antigens Class IIInterleukinvirus diseasesT helper cellT-Lymphocytes Helper-InducerOriginal ArticlesMiddle Agedbiology.organism_classificationVirology3. Good healthInterleukin-10Endotoxinsmedicine.anatomical_structureImmunologyLeukocytes MononuclearCapsid ProteinsFemaleCell Divisionmedicine.drugClinical and experimental immunology
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Reprogramming of Pericyte-Derived Cells of the Adult Human Brain into Induced Neuronal Cells

2012

SummaryReprogramming of somatic cells into neurons provides a new approach toward cell-based therapy of neurodegenerative diseases. A major challenge for the translation of neuronal reprogramming into therapy is whether the adult human brain contains cell populations amenable to direct somatic cell conversion. Here we show that cells from the adult human cerebral cortex expressing pericyte hallmarks can be reprogrammed into neuronal cells by retrovirus-mediated coexpression of the transcription factors Sox2 and Mash1. These induced neuronal cells acquire the ability of repetitive action potential firing and serve as synaptic targets for other neurons, indicating their capability of integrat…

AdultNeurogenesisCellular differentiationInduced Pluripotent Stem CellsAction PotentialsBiologySynaptic TransmissionMiceNeural Stem CellsSOX2Basic Helix-Loop-Helix Transcription FactorsGeneticsmedicineAnimalsHumansInduced pluripotent stem cellCells CulturedCerebral CortexNeuronsSOXB1 Transcription FactorsNeurogenesisCell DifferentiationNeurodegenerative DiseasesCell BiologyCellular ReprogrammingNeural stem cellCell biologyRetroviridaemedicine.anatomical_structureImmunologyMolecular MedicineNeuronPericyteNerve NetPericytesReprogrammingStem Cell TransplantationCell Stem Cell
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