Search results for "BBP"
showing 10 items of 16 documents
MAB2.0 project: Integrating algae production into wastewater treatment
2018
Abstract Different species of microalgae are highly efficient in removing nutrients from wastewater streams and are able to grow using flue gas as a CO2 source. These features indicate that application of microalgae has a promising outlook in wastewater treatment. However, practical aspects and process of integration of algae cultivation into an existing wastewater treatment line have not been investigated. The Climate-KIC co-funded Microalgae Biorefinery 2.0 project developed and demonstrated this integration process through a case study. The purpose of this paper is to introduce this process by phases and protocols, as well as report on the challenges and bottlenecks identified in the cas…
Opposing Effects of CREBBP Mutations Govern the Phenotype of Rubinstein-Taybi Syndrome and Adult SHH Medulloblastoma
2018
Recurrent mutations in chromatin modifiers are specifically prevalent in adolescent or adult patients with Sonic hedgehog-associated medulloblastoma (SHH MB). Here, we report that mutations in the acetyltransferase CREBBP have opposing effects during the development of the cerebellum, the primary site of origin of SHH MB. Our data reveal that loss of Crebbp in cerebellar granule neuron progenitors (GNPs) during embryonic development of mice compromises GNP development, in part by downregulation of brain-derived neurotrophic factor (Bdnf). Interestingly, concomitant cerebellar hypoplasia was also observed in patients with Rubinstein-Taybi syndrome, a congenital disorder caused by germline mu…
Tetrabromobisphenol A (TBBPA)-stimulated reactive oxygen species (ROS) production in cell-free model using the 2′,7′-dichlorodihydrofluorescein diace…
2016
t Tetrabromobisphenol A (TBBPA) is a widely used brominated flame retardant, applied in a variety of commercial and household products, mainly electronic ones. Since the production of reactive oxygen species (ROS) is considered one of the principal cytotoxicity mechanisms, numerous studies undertake that aspect of TBBPA’s mechanism of action. The present study verifies if the fluorogenic substrate 2′,7′- dichlorodihydrofluorescein diacetate (H2DCFDA) should be used to detect ROS production induced by TBBPA. To determine the ability of TBBPA alone to stimulate the conversion of H2DCFDA to its fluorescent product 2’, 7’- dichlorofluorescein (DCF), we used a cell-free model. In the experiments…
Histone acetylation deficits in lymphoblastoid cell lines from patients with Rubinstein-Taybi syndrome.
2012
Background: Rubinstein-Taybi syndrome (RSTS) is a congenital neurodevelopmental disorder defined by postnatal growth deficiency, characteristic skeletal abnormalities and mental retardation and caused by mutations in the genes encoding for the transcriptional co-activators with intrinsic lysine acetyltransferase (KAT) activity CBP and p300. Previous studies have shown that neuronal histone acetylation is reduced in mouse models of RSTS. Methods: The authors identified different mutations at the CREBBP locus and generated lymphoblastoid cell lines derived from nine patients with RSTS carrying distinct CREBBP mutations that illustrate different grades of the clinical severity in the spectrum …
Baktērijas Borrelia burgdorferi proteīna BBP28 strukturālie pētījumi
2017
Laima slimība ir visizplatītākā ar vektoriem pārnēsātā slimība Amerikas Savienotajās Valstīs. Neraugoties uz tās plašo izplatību un smagajiem slimības simptomiem, pret to vēl aizvien nav pieejama vakcīna. Darba mērķis bija ekspresēt Laima slimības izraisītājas, baktērijas (Borrelia burgdorferi), ar membrānu saistīto lipoproteīnu BBP28 un noteikt tā struktūru. Izmantojot KMR datus, tika aprēķinātas divas BBP28 proteīna struktūras - viena pilna garuma un otra saīsinātai proteīna sekvencei, kas nesatur nestrukturēto 27 aa garo N gala segmentu. Strukturēto BBP28 daļu veido piecas α-spirāles un 14 aa gara virkne C-galā, kas kovalenti piesaistīta ar disulfīda saiti pie 4. un 5. α-spirāli savienoj…
GUTTUSO E BBPR A PALERMO.
2009
PPAR-γ Agonist GW1929 But Not Antagonist GW9662 Reduces TBBPA-Induced Neurotoxicity in Primary Neocortical Cells
2013
Tetrabromobisphenol A (2,2-bis(4-hydroxy-3,5-dibromophenyl)propane; TBBPA) is a widely used brominated flame retardant. TBBPA induces neuronal damage, but the mechanism by which this occurs is largely unknown. We studied the possible involvement of peroxisome proliferator-activated receptor gamma (PPAR-γ) in TBBPA-induced apoptosis and toxicity in mouse primary neuronal cell cultures. TBBPA enhanced both, caspase-3 activity and lactate dehydrogenase (LDH) release in neocortical cells after 6 and 24 h of exposition. These data were supported at the cellular level with Hoechst 33342 staining. Immunoblot analyses showed that, compared with control cells, 10 μM TBBPA decreased the expression of…
I BBPR a Palermo tra innesti di modelli esogeni e influenze endogene
2021
Se vi è qualcosa di profondamente e visceralmente connaturato alla stessa dimensione esistenziale della città di Palermo, sin dalle sue molteplici e stratificate origini fenicio-puniche, questo è certamente il concetto di ‘innesto’. Ed in analogia con l’innesto agrario, cioè con la pratica del far concrescere in una pianta esistente una parte di un altro vegetale, al fine di rafforzare il primo soggetto ma modificandolo verso un genere diverso da quello iniziale, l’intera storia millenaria della città potrà essere riguardata come il frutto di continui, ciclici introduzioni di modelli architettonici e urbani esogeni, declinati rispetto alle contingenze culturali autoctone dei diversi esempi …
Venticinquesima Strada, Quarantuno Ovest. Dialogo ul Ventunesimo Blocco.
2012
Intorno a Glossa 21, e grazie alla visibilità data da Peter Eisenman, è iniziato un percorso condiviso e collettivo, che vuole superare diatribe politiche e che, oltre a salvare il Memoriale, permetta di arricchirlo di nuovi contributi. Percorso che si è avvalso, man mano, di nuovi appoggi, come la CGIL, l’Arcigay, il CNJ e un sempre maggior numero di cittadini che ora arrderiscono e appoggiano ufficialmente il Progetto Glossa 21. Una cittadinanza attiva che vede in questa battaglia qualcosa che va oltre la semplice salvaguardia di un Memoriale e cioè la difesa dei valori contenuti in un’opera laicamente sacra in un terreno intoccabile come quello di Auschwitz-Birkenau. Una battaglia che va…