Search results for "BED"

Expression and purification of polyhistidine-tagged firefly luciferase in insect cells

2001

The coleopteran firefly, Photinus pyralis, luciferase was produced in lepidopteran Trichoplusia ni insect cells using a baculovirus expression vector. The recombinant protein was equipped with a polyhistidine affinity tag at the carboxyl terminus and purified by immobilized metal-ion affinity chromatography in combination with an expanded bed adsorption system. This approach enabled an efficient, one-step purification protocol of a genetically modified luciferase with properties similar to those of the authentic counterpart. According to light emission measurements, the final yield of highly purified protein was 23 mg l−1 of cell culture. In addition, no specific interaction of interfering …

Il Bed & Breakfast

2015

bed & breakfast

Nonuniform sediment transport estimation in non-equlibrium situations: study cases

2014

Photogrammetric survey to measure the bed topography of a laboratory large amplitude meandering channel

2010

Temporal Evolution of Scour downstream bed sills

2007

Empirical estimation of maximum scour depth downstream bed sills

2009

Experimental Observation Of Turbulent Structures In A Straight Flume

2010

Experimental and theoretical studies of the structure of turbulence in open-channel flows have shown that the dynamics of the wall layer turbulence is dominated by the formation and growth of turbulent structures which evolve periodically as part of the so-called bursting phenomena. In the present paper experimental results obtained in a straight flume for different roughness conditions of the channel walls are described. The occurrence of turbulent events is verified by applying the conditioned quadrant analysis. The information about the spatial and temporal scales of the events is obtained through the space-time correlations of the conditioned data.

A study on The Factors Affecting Merger and Acquisition Decision In Nepalese Banking Sector

2016

Master's thesis Business Administration BE501 - University of Agder 2016 The main purpose of this study is to find out the factors that would lead to making decision regarding going for merger and acquisition among the banks in Nepalese banking sector. This thesis finds out various factors that would affect merger and acquisition decision in banking sector based upon previous studies, and tries to check whether these are significant in case of Nepalese banking sector or not. Four theories: neo-classical, agency, behavioral and resource dependency; have been taken into consideration for purpose of finding out factors affecting decision to go for merger and acquisition. Based upon the previou…

Assessment of the Trend of Albedo: a Case Study of Palermo

2013

In this paper we propose a case study of urban heat island applied to Palermo. The urban heat island (UHI) is the most studied of the climate effects of settlements. The UHI refers to the generally warm urban temperatures compared to those over surrounding, non-urban, areas. The aim of this paper is to find a connection among the average rise in temperature and the modification of albedo.

The monophyly and evolution of Cynara L. (Asteraceae) sensu lato: evidence from the Internal Transcribed Spacer region of nrDNA

2005

The monophyly and evolution of Cynara was investigated using ITS sequence data. Parsimony analysis supports the monophyly of Cynara sensu lato, i.e. including the distinctive taxa C. humilis and C. tournefortii. This contradicts the recent decision to create a new monotypic genus Arcyna for C. tournefortii. A hypothesised close relationship between C. tournefortii and Silybum Adans. is also refuted. Four of the five species of Cynara, for which multiple accessions were sequenced, were shown to be monophyletic but C. baetica was found to be non-monophyletic. Free energy estimates for ITS1 secondary structure and conservation of the 5.8S region suggest that this is not due to the occurrence o…