Search results for "BED"
showing 10 items of 1605 documents
Miradas oblicuas : infancias, paisajes y comunidades interrumpidas en el cine de Dominga Sotomayor
2020
Dominga Sotomayor propone, en De jueves a domingo (2012), Mar (2014) y Tarde para morir joven (2018), exploraciones de la intimidad de una familia, una pareja y una comunidad. En estos acercamientos, la intimidad se configura en grados variables de tensión con el afuera y lo público. En estas oscilaciones se pone en evidencia una de las características más recurrentes y estudiadas del cine chileno contemporáneo: la conversión de la intimidad en el locus sobre el que se proyectan, de manera indirecta, los conflictos del pasado y el presente. Este artículo considera la forma en que el cine de Sotomayor se inscribe en el panorama actual del cine chileno y, a través de él, en su escenario socio…
PASSENGER SATISFACTION WITH HYBRID ELECTRIC BUS SERVICE IN NORWAY : A PLS Structural Equation Modelling Approach
2019
Master's thesis Business Administration BE501 - University of Agder 2019 The use of hybrid electric buses (HEBs) in public transit is one of the global attention received topics in this 21st century because of its environment friendly nature. This study focusses on effect of bus service quality dimensions such as bus driver's quality, bus tangible and empathy to determine customer satisfaction level of bus service in Kristiansand region. Structural equation model is used for an empirical investigation to find out the association of perceived bus service quality dimensions with word of mouth mediated by perceived value and customer satisfaction. A total of 276 valid university student respon…
Synthesizing on a reconfigurable chip an autonomous robot image processing system
2003
This paper deals with the implementation, in a high density reconfigurable device, of an entire log-polar image processing system. The log-polar vision reduces the amount of data to be stored and processed, simplifying several vision algorithms and making it possible the implementation of a complete processing system on a single chip. This image processing system is specially appropriated for autonomous robotic navigation, since these platforms have typically power consumption, size and weight restrictions. Furthermore, the image processing algorithms involved are time consuming and many times they have also real-time restrictions. A reconfigurable approach on a single chip combines hardwar…
LC-MS/MS metodes izstrāde otrās izvēles prettuberkulozes zāļu vielu un to metabolītu kvantitatīvai noteikšanai asins plazmā
2022
LC-MS/MS metodes izstrāde otrās izvēles prettuberkulozes zāļu vielu un to metabolītu kvantitatīvai noteikšanai asins plazmā. Gukālova B., zinātniskā vadītāja Dr. chem. Grīnberga S. Maģistra darbs, 52 lappuses, 12 attēli, 11 tabulas, 7 pielikumi, 56 literatūras avoti. Latviešu valodā. Darbā apkopota literatūra par LC-MS/MS pielietošanas iespējām otrās izvēles prettuberkulozes zāļu vielu un to metabolītu kvantitatīvai noteikšanai, raksturotas bioloģisko paraugu sagatavošanas metodes. Darba gaitā tika pārbaudītas dažādas paraugu sagatavošanas metodes, veikta hromatogrāfisko un masspektrometrisko apstākļu pielāgošana kvantitatīvai prettuberkulozes zāļu un to metabolītu noteikšanai asins plazmā.…
Diversity of rhizobia nodulating wild shrubs of Sicily and some neighbouring islands
2008
Abstract Legume shrubs have great potential for rehabilitation of semi-arid degraded soils in Mediterranean ecosystems as they establish mutualistic symbiosis with Nfxing rhizobia. Eighty-eight symbiotic rhizobia were isolated from seven wild legume shrubs native of Sicily (Southern Italy) and grouped in operational taxonomic units (OTU) by analysis of the ribosomal internal transcribed spacer (ITS) polymorphism. Partial sequencing of 16S rRNA gene of representative isolates of each OTU revealed that most Genisteae symbionts are related to Bradyrhizobium canariense, B. japonicum and B. elkanii. Teline monspessulana was the only Genistea nodulated by Mesorhizobium strains, and Anagyris foeti…
PCR testing for Treponema pallidum in paraffin-embedded skin biopsy specimens: test design and impact on the diagnosis of syphilis
2007
Background: Syphilis, a chronic infection caused by Treponema pallidum (T. pallidum), is a disease which is increasing in incidence, and thus more and more becoming a differential diagnosis in routine pathology. Aim: Since histological changes are not specific, we sought to develop a polymerase chain reaction (PCR)-based molecular assay for the detection of T. pallidum in formalin-fixed, paraffin-embedded tissues, and evaluate its diagnostic power, especially in comparison with other ancillary methods, i.e. immunohistochemistry and Dieterle staining. Methods: 36 skin biopsies with the clinical and /or serological diagnosis of syphilis were evaluated by morphology, immunohistochemistry and s…
Comparison of two PCR methods for detection of Leptospira interrogans in formalin-fixed and paraffin-embedded tissues
2012
In this study we compared two polymerase chain reaction (PCR) methods using either 16S ribosomal RNA (rRNA) or 23S rRNA gene primers for the detection of different Leptospira interrogans serovars. The performance of these two methods was assessed using DNA extracted from bovine tissues previously inoculated with several bacterial suspensions. PCR was performed on the same tissues before and after the formalin-fixed, paraffin-embedding procedure (FFPE tissues). The 23S rDNA PCR detected all fresh and FFPE positive tissues while the 16S rDNA-based protocol detected primarily the positive fresh tissues. Both methods are specific for pathogenic L. interrogans. The 23S-based PCR method successfu…
Fast protocols for the 5S rDNA and ITS-2 based identification ofOenococcus oeni
2005
To identify specific marker sequences for the rapid identification of Oenococcus oeni, we sequenced the 23S-5S internal transcribed spacer (ITS-2) region and the 5S rDNA of five different O. oeni strains and three phylogenetically related lactic acid bacteria (LAB). Comparative analysis revealed 100% identity among the ITS-2 region of the O. oeni strains and remarkable differences in length and sequence compared to related LAB. These results enabled us to develop a primer set for a rapid PCR-identification of O. oeni within three hours. Moreover, the comparison of the 5S rDNA sequences and the highly conserved secondary structure provided the template for the design of three fluorescence-la…
Differential cycles of range contraction and expansion in European high mountain plants during the Late Quaternary: insights from Pritzelago alpina (…
2003
Nuclear DNA sequence variation of the internal transcribed spacer (ITS) and amplified fragment length polymorphisms (AFLPs) were used to illuminate the evolutionary history of Pritzelago alpina, a herbaceous perennial of (sub)alpine to nival habitats of the European high mountains. Maximum likelihood analysis of ITS sequences of P. alpina, Hornungia petraea and Hymenolobus procumbens (the 'Pritzelago alliance') resolved P. alpina and H. petraea as sister taxa. ITS divergence estimates support an origin for P. alpina in the Late Tertiary, while intraspecific diversification started in the Late Quaternary (0.4-0.9 million years ago). AFLP analysis of 76 individuals of P. alpina, representing …
Nuclear ribosomal DNA (nrDNA) concerted evolution in natural and artificial hybrids of Armeria (Plumbaginaceae)
1999
Nuclear ribosomal DNA (nrDNA) internal transcribed spacer (ITS) sequences from artificial hybrids and backcrosses between Armeria villosa ssp. longiaristata and A. colorata were studied to assess the possible effects of concerted evolution in natural hybrids. F1 artificial hybrids show the expected pattern of additive polymorphisms for five of the six variable sites as estimated from direct sequences. However, homogenization of polymorphism is already observed in the F2, and is biased towards A. colorata except for one site. In backcrosses, an expected tendency towards homogenization of polymorphic sites in the direction of the recurrent parent is observed for five sites, although this does…