Search results for "BLAST"
showing 10 items of 2136 documents
Cancer in the first 18 months of life
2020
Introduction: Oncological-haematological disease continues to be the first cause of non-traumatic mortality in childhood, as well as a significant cause of morbidity. The patient less than 18-months-old has special clinical, diagnostic, and therapeutic features that all paediatricians are interested in determining, with the aim of achieving greater survival and a lower morbidity throughout the lives of their patients. Material and methods: A retrospective, descriptive study was carried out using the clinical, diagnostic, and therapeutic variables in patients less than 18-months-old diagnosed with an oncological-haematological that received chemotherapy in a Paediatric Oncology Unit between …
Gingival fibroblasts ?in vitro? and Down's Syndrome
2012
Gingival fibroblast cultures from four patients with Down's Syndrome (DS) and periodontal disease were compared with four in vitro age-matched fibroblast cultures of handicapped subjects (ND) also affected by periodontitis. The extra copy of cromosome 21 could alter growth regulation and biochemical mechanisms, so we examined quantitatively some DS phenotypical aspects to detect possible differences from those of controls. The growth properties of gingival fibroblast cultures from DS patients were more elevated than their ND age-matched controls. There were no differences in plasma membrane polarization and in neutral endopeptidase activity. The succinate-cytochrome C reductase activity dec…
Regulation of Anti-Apoptotic SOD2 and BIRC3 in Periodontal Cells and Tissues.
2021
Made available in DSpace on 2021-06-25T10:49:07Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-01-02 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Deutscher Akademischer Austauschdienst Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Deutsche Forschungsgemeinschaft The aim of the study was to clarify whether orthodontic forces and periodontitis interact with respect to the anti-apoptotic molecules superoxide dismutase 2 (SOD2) and baculoviral IAP repeat-containing protein 3 (BIRC3). SOD2, BIRC3, and the apoptotic markers caspases 3 (CASP3) and 9 (CASP9) were analyzed in gingiva from periodontally healthy and periodontitis subjects by real-time PCR…
Influence of Agents Used for Topical Wound Treatment on Phagocyte Stimulation and Fibroblast Growth
1995
Wound healing has always been a fascinating phenomenon for physicians and surgeons. Wounds usually close by forming new granulation tissue, contracting, and re-epithelializing. Before granulation begins, polymor-phonuclear leukocytes (PMNs) and monocytes/macrophages (MCs) are chemotactically attracted to sites of injury. Upon contact with various stimuli in the wound including bioactive lipids, complement components, certain cytokines, e.g., tumor necrosis factor-α (TNF-α) [1], granulocyte/ monocyte-colony stimulating factor (GM-CSF), interferon-γ (IFN-γ), and interleukin-8, and particles such as microorganisms, phagocytes respond by a respiratory burst consisting of a markedly enhanced upt…
Multiple Receptors Mediate apoJ-Dependent Clearance of Cellular Debris into Nonprofessional Phagocytes
2001
Phagocytosis of apoptotic, senescent, and dying cells by macrophages is a well characterized process. More recently it has been shown that in addition to macrophages vital neighboring cells in the affected tissue participate in the cellular clearance. While scavenger receptors have been shown to mediate uptake into macrophages, it is poorly understood how cellular debris is internalized by nonprofessional phagocytes. We here analyze the endocytic activity of vital fibroblasts and epithelial cells exposed to cellular debris and membrane remnants. We show a mutual stimulation in the endocytosis of debris and apolipoproteinJ (clusterin) in these cells. Experiments using RAP (receptor-associate…
Ricerche sulla induzione del sistema nervoso nelle larve delle Ascidie
1959
Summary Phallusia mamillata embryos at the 8-, 16-, 32- and 61-cell stages have been deprived of determined vegetative anterior blastomeres (deriving from, the A4.1 cells), with the purpose to sec which influence is exerted by such blastomeres in the induction of the nervous system. The results have been the following. 1 — The nervous system is not yet determined at the 64-cell stage. 2 — A neurali induction can be exerted even by a few blastomeres of the 64-ccll stage, containing cither entodermic or chordal material. 3 — Such inducing blastomeres arc those which give rise to the archenteron roof of the gastrula.
Dis-organizing centrosomal clusters: specific cancer therapy for a generic spread?
2015
Cancer is a leading cause of mortality and the annual incidence of new cancer cases is rising worldwide. Due to the frequent development of resistance and the side effects of established anti-cancer drugs, the quest for new drugs with improved therapeutic features goes on. In contrast to cytotoxic chemotherapy of the past, the concept of targeted chemotherapy attempts to increase specificity of therapy by attacking tumor-related mechanisms. A novel emerging treatment concept represents the inhibition of centrosomal clustering. The centrosome regulates mitotic spindle formation assuring uniform separation of chromosomes to daughter cells. Many tumors contain supernumerary centrosomes, which …
Up-regulation of cholesterol associated genes as novel resistance mechanism in glioblastoma cells in response to archazolid B
2014
Treatment of glioblastoma multiforme (GBM), the most common and aggressive lethal brain tumor, represents a great challenge. Despite decades of research, the survival prognosis of GBM patients is unfavorable and more effective therapeutics are sorely required. Archazolid B, a potent vacuolar H(+)-ATPase inhibitor influencing cellular pH values, is a promising new compound exerting cytotoxicity in the nanomolar range on wild-type U87MG glioblastoma cells and U87MG.∆EGFR cells transfected with a mutant epidermal growth factor receptor (EGFR) gene. Gene expression profiling using microarray technology showed that archazolid B caused drastic disturbances in cholesterol homeostasis. Cholesterol,…
Distribution of protease activity in the blastula and early gastrula ofDiscoglossus pictus
1957
E stata determinata l'attivita proteasica nelle differenti regioni della blastula e della gastrula diDiscoglossus pictus. Nella blastula non esiste una differenza di attivita fra meta animale e vegetativa, quando si prenda come riferimento l'azoto citoplasmatico. Nella giovane gastrula il territorio presuntivo della corda e del sistema nervoso mostra una attivita piu elevata della epidermide presuntiva.
The rates of adult neurogenesis and oligodendrogenesis are linked to cell cycle regulation through p27-dependent gene repression of SOX2
2023
Abstract Cell differentiation involves profound changes in global gene expression that often have to occur in coordination with cell cycle exit. Because cyclin-dependent kinase inhibitor p27 reportedly regulates proliferation of neural progenitor cells in the subependymal neurogenic niche of the adult mouse brain, but can also have effects on gene expression, we decided to molecularly analyze its role in adult neurogenesis and oligodendrogenesis. At the cell level, we show that p27 restricts residual cyclin-dependent kinase activity after mitogen withdrawal to antagonize cycling, but it is not essential for cell cycle exit. By integrating genome-wide gene expression and chromatin accessibil…