Search results for "BLAST"
showing 10 items of 2136 documents
Engineering a morphogenetically active hydrogel for bioprinting of bioartificial tissue derived from human osteoblast-like SaOS-2 cells.
2014
Abstract Sodium alginate hydrogel, stabilized with gelatin, is a suitable, biologically inert matrix that can be used for encapsulating and 3D bioprinting of bone-related SaOS-2 cells. However, the cells, embedded in this matrix, remain in a non-proliferating state. Here we show that addition of an overlay onto the bioprinted alginate/gelatine/SaOS-2 cell scaffold, consisting of agarose and the calcium salt of polyphosphate [polyP·Ca 2+ -complex], resulted in a marked increase in cell proliferation . In the presence of 100 μ m polyP·Ca2+ -complex, the cells proliferate with a generation time of approximately 47–55 h. In addition, the hardness of the alginate/gelatin hydrogel substantially i…
Porous Gelatin Hydrogels: 2. In Vitro Cell Interaction Study
2007
We report on the feasibility of applying porous gelatin hydrogels, prepared by a novel and controlled cryogenic treatment, as cell-interactive scaffolds for tissue engineering applications. Despite the large number of publications on gelatin as a biomaterial, a detailed study of screening a limited number of gelatin scaffolds for their interaction with a panel of human cells has, to the best of our knowledge, not yet been published. In the present work, we have evaluated two types of porous gelatin scaffolds that differ in their pore geometry and pore size. Type I hydrogels contained top-to-bottom transverse channels (i.e. cones) with a decreasing diameter from the top (330 microm) to the b…
A morphometric comparison of dissimilar early development in sibling species of Platynereis (Annelida, Polychaeta)
1992
Early development of Platynereis massiliensis was studied in serial sections of fixed embryos and in living or fixed embryos whose nuclei had been made visible with a fluorescent label. The unfertilized egg is an ellipsoid with three axes of differing length. The longest axis corresponds to the dorsoventral axis of the developing embryo. Egg volume is ten times that in the sibling species, P. dumerilii, mainly due to increased yolk content. The timing and spatial pattern of cleavage were observed from first cleavage to the 62-cell stage. Volumes of the blastomeres, their nuclei, their yolk-free cytoplasm and their yolk were determined from serial sections up to the 29-cell stage. In the P. …
Development of antibiotic loaded biodegradable matrices to prevent superficial infections associated to total knee arthroplasty.
2019
Abstract Development of a pharmaceutical form for the superficial infections related with arthroplasties would be helpful for clinical practice. In this context, we set out to evaluate ciprofloxacin and gentamicin elution from systems based on chitosan. Films and semisolid hydrogels containing chitosan alone (2%) or in combination with gelatin (6%) or different proportions (from 12% to 36%) of tetrakis-(hydroxymethyl)-phosphonium-chloride (THPC) were tested as delivery systems. Different antibiotic doses were assayed (0.5 mg/cm2,1 mg/cm2 and 2 mg/cm2). Antibiotic release was studied for each formulation. In vitro cytocompatibility studies and a simulation exercise for bioactivity evaluation…
Spatial and temporal pattern of neuroblasts, proliferation, and Engrailed expression during early brain development in Tenebrio molitor L. (Coleopter…
2003
Abstract In insects, the knowledge of embryonic brain development is still fragmentary, and comparative data are scarce. In this study, we explored aspects of embryonic brain development in the coleopteran Tenebrio molitor . A detailed description is provided of the spatial and temporal pattern of the embryonic brain neuroblasts during 18–60% of embryonic development. Approximately 125 brain NBs have been identified in each hemisphere of the brain at about 40% of embryonic development. A subset of five neuroblasts, among them the two progenitors of the mushroom bodies and two progenitors of the larval antennal lobe, are morphologically identifiable by their larger size. As revealed by incor…
Probing the self-assembly and anti-glioblastoma efficacy of a cinnamoyl-capped dipeptide hydrogelator
2022
Herein, we introduce the first diphenylalanine dipeptide hydrogelator capped with the cinnamoyl functional group (Cin-L-F-L-F). We evaluate the effects of the cinnamoyl moiety on molecular self-assembly events and resultant physical properties of the hydrogel formed. In addition, we report our preliminary results of this dipeptide's cytotoxicity against glioblastoma (GBM) cancer cells. peerReviewed
Gene expression in TGFbeta-induced epithelial cell differentiation in a three-dimensional intestinal epithelial cell differentiation model
2006
Abstract Background The TGFβ1-induced signal transduction processes involved in growth and differentiation are only partly known. The three-dimensional epithelial differentiation model, in which T84 epithelial cells are induced to differentiate either with TGFβ1 or IMR-90 mesenchymal cell-secreted soluble factors, is previously shown to model epithelial cell differentiation seen in intestine. That model has not been used for large scale gene expression studies, such as microarray method. Therefore the gene expression changes were studied in undifferentiated and differentiated three-dimensional T84 cultures with cDNA microarray method in order to study the molecular changes and find new play…
Cytotoxicity of a new hemostatic agent on human pulp fibroblasts in vitro
2011
Objective: The objective of this study was to evaluate the cytotoxicity of the plant extract ankaferd blood stopper (ABS) in vitro. Study Design: ABS was eluted with fresh Dulbecco's Modified Eagle's Medium (DMEM) without serum for 72 h, at 37°C. The cells treated with various dilutions of ABS were seeded into 96-well microplate at 10 4 /well in triplicates. Cells without treatment served as a control group. The number of viable cells after 48 h incubation was determined by a modified 3-(4, 5-dimethyl-thiazol-2-yl)-2, 5-diphenyl-tetrazolium bromide (MTT) assay. The relative viability of pulp cells was expressed as color intensity of the number in the experimental wells relative to that of t…
Enteroparasitosis asociadas a trastornos intestinales crónicos
2021
Las enteroparasitosis afectan a la estructura y ambiente intestinal generando sintomatología en pacientes susceptibles. Éstas podrían llegar a desencadenar, agravar o confundirse con Trastornos Gastrointestinales Crónicos (TGC), cada vez más frecuentes en la población. En el presente estudio, se analiza la prevalencia de parásitos intestinales en 295 pacientes con TGC y/o sintomatología mediante la combinación de métodos diagnósticos. Giardia intestinalis fue el parásito más prevalente (61,7%), seguido de Blastocystis sp. (13,2%), con una prevalencia de parasitación general del 67%. El análisis molecular halló los genotipos A (96%) y B (4%) de G. intestinalis, y cinco subtipos de Blastocyst…
Gēnu ekspresija glioblastomas pacientiem ar atšķirīgu dzīvildzi
2015
Glioblastomu raksturošana molekulārā līmenī ar gēnu ekspresijas mikročipiem ir aktuāla metode mūsdienās, lai identificētu jaunus pretvēža terapijas mērķus. Darba mērķis bija salīdzināt divas pacientu grupas ar atšķirīgu dzīvildzi un noteikt atšķirīgi ekspresētos gēnus. Gēnu ekspresijas analīzi veica ar mikročipu tehnoloģiju, salīdzinot divas grupas. Negatīvā grupā sastāvēja no 13 pacientiem, kam dzīvildze bija īsāka par 6 mēnešiem, un pozitīvā - 15 pacienti ar dzīvildzi virs 6 mēnešiem. Glioblastomu RNS tika izdalīts no formalīnā fiksētiem, parafīnā guldītiem audiem. Iegūtos datus apstrādāja, izmantojot statistiskās analīzes metodes. Rezultātā noteica 30 dažādi ekspresēti gēni, no kuriem iz…