Search results for "BODY"
showing 10 items of 6956 documents
Evaluation of HBs, HBc, and frCP virus-like particles for expression of human papillomavirus 16 E7 oncoprotein epitopes.
2002
<i>Objectives:</i> In an attempt to develop virus-like particles (VLPs) as experimental vaccine against human papilloma virus (HPV)-induced tumours, the HPV16 E7 oncoprotein epitopes spanning amino acid (aa) residues 35–98 were expressed on three proteins capable of VLP formation: hepatitis B virus (HBV) surface (HBs) and core (HBc) antigens, and RNA phage fr coats (frCP). <i>Methods:</i> The profile of immunoglobulin isotypes induced in Balb/C mice after immunization with purified chimeric proteins was studied. <i>Results:</i> The HBs*-E7(35–54) protein expressing E7 residues 35–54 between residues 139 and 142 of the HBs carrier formed HBs-like particles…
Molecular Basis of SARS-CoV-2 Nsp1-Induced Immune Translational Shutdown as Revealed by All-Atom Simulations.
2021
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic represents the most severe global health crisis in modern human history. One of the major SARS-CoV-2 virulence factors is nonstructural protein 1 (Nsp1), which, outcompeting with the binding of host mRNA to the human ribosome, triggers a translation shutdown of the host immune system. Here, microsecond-long all-atom simulations of the C-terminal portion of the SARS-CoV-2/SARS-CoV Nsp1 in complex with the 40S ribosome disclose that SARS-CoV-2 Nsp1 has evolved from its SARS-CoV ortholog to more effectively hijack the ribosome by undergoing a critical switch of Q/E158 and E/Q159 residues that perfects Nsp1's interactions…
Lipid-related thiamine deficiency cause mortality of river lampreys (Lampetra fluviatilis) during pre-spawning fasting
2023
Publisher Copyright: © 2023 The Authors River lampreys (Lampetra fluviatilis) were caught in the fall 2014 on entering the River Perhonjoki for spawning and kept at a hatchery until spawning in late spring 2015 to produce larvae for compensatory stockings. Since the lampreys died massively from early February onwards, they were investigated in March and May to clarify the cause of the deaths. The symptoms in lampreys resembled those of lipid-related thiamine (vitamin B1) deficiency of salmonines, called the M74 syndrome in the Baltic Sea area. Because the lipid content of lampreys was known to be high, thiamine concentrations were analyzed in the liver and ovulated unfertilized eggs, and th…
Does using a visual-representation tool foster students’ ability to identify forces and construct free-body diagrams?
2013
Earlier research has shown that after physics instruction, many students have difficulties with the force concept, and with constructing free-body diagrams (FBDs). It has been suggested that treating forces as interactions could help students to identify forces as well as to construct the correct FBDs. While there is evidence that identifying interactions helps students in quantitative problem solving, there is no previous research investigating the effect of a visual-representation tool—an interaction diagram (ID)—on students’ ability to identify forces, and to construct the correct FBDs.We present an empirical study conducted in 11 Finnish high schools on students (n ¼ 335, aged 16) takin…
Anticipatory Postural Adjustments and kinematic arm features when postural stability is manipulated
2018
International audience; Beyond the classical paradigm that presents the Anticipatory Postural Adjustments (APAs) as a manner to create forces that counteract disturbances arising from the moving segment during a pointing task, there is a controversial discussion about the role APAs to facilitate the movement and perform a task accurately. In addition, arm kinematics features are classically used to infer the content of motor planning for the execution and the control of arm movements, The present study aimed to disentangle the conflicting role of APAs during an arm-pointing task in which the subjects reach a central diode that suddenly turns on, while their postural stability was manipulate…
Development of an immunochromatographic assay based on carbon nanoparticles for the determination of the phytoregulator forchlorfenuron
2013
Rapid analytical methods enabling the determination of diverse targets are essential in a number of research areas, from clinical diagnostics to feed and food quality and safety. Herein, the development of a quantitative immunochromatographic assay for the detection of the synthetic phytoregulator forchlorfenuron (CPPU) is described. The competitive lateral flow immunoassay (LFIA) was based on the immobilization onto a nitrocellulose membrane of an ovalbumin-CPPU conjugate (test line) and on the use of an immunodetection ligand consisting of carbon nanoparticles labeled with an anti-CPPU monoclonal antibody through interaction with a secondary antibody. The presence of CPPU in horticultural…
The mechanically based non-local elasticity: an overview of main results and future challenges
2013
The mechanically based non-local elasticity has been used, recently, in wider and wider engineering applications involving small-size devices and/or materials with marked microstructures. The key feature of the model involves the presence of non-local effects as additional body forces acting on material masses and depending on their relative displacements. An overview of the main results of the theory is reported in this paper.
Anatomiczny spektakl w dawnej poezji erotycznej
2016
Artykuł stanowi próbę deskrypcji dawnych zabiegów medycznych, takich jak sekcja zwłok, w kontekście kulturowo-literackiego wydarzenia popularnego w XVI- i XVII-wiecznej Europie. W tekście przywołano genezę padewskich spotkań o charakterze theatrum anatomicum, z dokładnym opisem najważniejszych rytuałów, procedur oraz okoliczności w ich organizowaniu. Autorka, korzystając z bogatego studium Anny Wieczorkiewicz Muzeum ludzkich ciał oraz szkiców autorstwa Radosława Grześkowiaka zatytułowanych Amor Curiosus. Szkice o osobliwych tematach dawnej poezji erotycznej, buduje swój wywód na użytej w tych pozycjach naukowych nazwie „spektakl anatomiczny”. Pozwala to na zgłębienie dychotomii sensu tychże…
Wolność pracy jako przesłanka ograniczająca swobodę kontraktowania - wybrane problemy
2022
Zgodnie z art. 65 ust. 1 Konstytucji RP każdemu zapewnia się wolność wyboru i wykonywania zawodu oraz wyboru miejsca pracy. Wpływ wyrażonej we wskazanym przepisie wolności pracy na swobodę kształtowania umownych stosunków pracy jest wciąż otwartym polem do dyskusji prawniczej. W prezentowanym tekście autorzy zmierzają do rozstrzygnięcia, czy zasada wolności pracy może zostać uznana za przesłankę ograniczającą swobodę kontraktowania. Zagadnienie to, mające charakter dogmatyczny, poddane zostało analizie systemowej. Autorzy przyjmują, że z perspektywy stosunków prywatnoprawnych wolność pracy należy ujmować jako element obiektywnego, preferowanego porządku wartości społeczno-gospodarczych (zas…
Longitudinal physical activity patterns and the development of cardiometabolic risk factors during adolescence
2023
Purpose To examine the associations between longitudinal physical activity (PA) patterns and the development of cardiometabolic risk factors from adolescence to young adulthood. Methods This cohort study encompassed 250 participants recruited from sports clubs and schools, and examined at mean age 15 and 19. Device-measured moderate-to-vigorous PA was grouped into five patterns (via a data-driven method, using inactivity maintainers as a reference). The outcomes were: glucose, insulin, homeostasis model assessment for insulin resistance (HOMA-IR), total cholesterol, HDL and LDL cholesterol, triglycerides, blood pressure, and body mass index (BMI). Linear growth curve models were applied wit…