Search results for "BPO"
showing 10 items of 43 documents
Morphometric and kinematic sperm subpopulations in split ejaculates of normozoospermic men
2016
This study was designed to analyze the sperm kinematic and morphometric subpopulations in the different fractions of the ejaculate in normozoospermic men. Ejaculates from eight normozoospermic men were collected by masturbation in three fractions after 3-5 days of sexual abstinence. Analyses of sperm motility by computer-assisted sperm analysis (CASA-Mot), and of sperm morphometry by computer-assisted sperm morphometry analysis (CASA-Morph) using fluorescence were performed. Clustering and discriminant procedures were performed to identify sperm subpopulations in the kinematic and morphometric data obtained. Clustering procedures resulted in the classification of spermatozoa into three kine…
Role of T-Cell Polarization and Inflammation and Their Modulation by n-3 Fatty Acids in Gestational Diabetes and Macrosomia.
2015
Th (T helper) cells are differentiated into either Th1 or Th2 phenotype. It is generally considered that Th1 phenotype is proinflammatory, whereas Th2 phenotype exerts anti-inflammatory or protective effects. Gestational diabetes mellitus (GDM) has been associated with a decreased Th1 phenotype, whereas macrosomia is marked with high expression of Th1 cytokines. Besides, these two pathological situations are marked with high concentrations of inflammatory mediators like tumor necrosis factor-α(TNF-α) and interleukin-6 (IL-6), known to play a pivotal role in insulin resistance. Dietary n-3 polyunsaturated fatty acids (n-3 PUFAs) may exert a beneficial effect by shifting Th1/Th2 balance to a …
EV-TRACK: transparent reporting and centralizing knowledge in extracellular vesicle research
2017
We argue that the field of extracellular vesicle (EV) biology needs more transparent reporting to facilitate interpretation and replication of experiments. To achieve this, we describe EV-TRACK, a crowdsourcing knowledgebase (http://evtrack.org) that centralizes EV biology and methodology with the goal of stimulating authors, reviewers, editors and funders to put experimental guidelines into practice.
HLA-B8,DR3 haplotype affects lymphocyte blood levels
1997
The number of lymphocytes in the blood is constant, pointing to an effective control of circulating lymphocyte values. The mechanisms of this regulation are uncertain, although it is likely that the number of blood lymphocytes is conditioned by hormones, homing factors and cytokines whose production is at least partly restrained by genetic factors. Particularly genetic factors linked to major histocompatibility complex (MHC) appear to be involved. In human beings a decreased number of blood lymphocytes has been described in healthy subjects carrying the Human Leucocyte Antigens (HLA) haplotype HLA-B8,DR3. In the present study, to inquire into the mechanisms of this lymphocyte decreased numb…
Less but better: cardioprotective lipid profile of patients with GCK-MODY despite lower HDL cholesterol level
2014
Patients with diabetes caused by single-gene mutations generally exhibit an altered course of diabetes. Those with mutations of the glucokinase gene (GCK-MODY) show good metabolic control and low risk of cardiovascular complications despite paradoxically lowered high-density lipoprotein (HDL) cholesterol levels. In order to investigate the matter, we analyzed the composition of low-density lipoprotein (LDL) and HDL subpopulations in such individuals. The LipoPrint(©) system (Quantimetrix, USA) based on non-denaturing, linear polyacrylamide gel electrophoresis was used to separate and measure LDL and HDL subclasses in fresh-frozen serum samples from patients with mutations of glucokinase or …
A 45-year sub-annual reconstruction of seawater temperature in the Bay of Brest, France, using the shell oxygen isotope composition of the bivalve Gl…
2020
A reconstruction of sea surface temperature (SST) spanning 45 years (1966–2011) was developed from δ18O obtained from the aragonitic shells of Glycymeris glycymeris, collected from the Bay of Brest, France. Bivalve sampling was undertaken monthly between 2014 and 2015 using a dredge. In total, 401 live specimens and 243 articulated paired valves from dead specimens were collected, of which 24 individuals were used to reconstruct SST. Temperatures determined using the palaeotemperature equation of Royer et al. compared well with observed SST during the growing season between 1998 and 2010 (Pearson’s correlation: p = 0.002, r = 0.760). Furthermore, a significant negative correlation was foun…
Dubultspiralizēto RNS modulējošās īpašības perifēro asiņu mononukleārās šūnās un audzēju šūnās
2016
Dubultspiralizētā RNS (dsRNS) darbojas kā iedzimtās un adaptīvās imūnatbildes aktivators. Larifāns ir Latvijā ražots farmaceitisks produkts, kas satur bakteriofāga izcelsmes dsRNS molekulas. PolyI:C ir viens no plaši pētītiem dsRNS sintētiskajiem analogiem. Bakalaura darba mērķis bija noraksturot dsRNS inducēto citokīnu spektru un limfocītu subpopulācijas ex vivo kultivētās mononukleārās šūnās, kā arī izvērtēt dsRNS ietekmi uz vēža šūnu mitotisko aktivitāti. Abas darbā analizētās dsRNS neizraisīja statistiski būtiskas izmaiņas galvenajās limfocītu populācijās, toties palielināja CD95, CD38, IFN-γ ekspresiju. Novēroja 13 dažādu molekulu indukciju, kā arī IL-16 supresiju Larifāna un PolyI:C k…
The Emerging Role of the Endocannabinoid System in Endocrine Regulation and Energy Balance
2005
During the last few years, the endocannabinoid system has emerged as a highly relevant topic in the scientific community. Many different regulatory actions have been attributed to endocannabinoids, and their involvement in several pathophysiological conditions is under intense scrutiny. Cannabinoid receptors, named CB1 receptor and CB2 receptor, first discovered as the molecular targets of the psychotropic component of the plant Cannabis sativa, participate in the physiological modulation of many central and peripheral functions. CB2 receptor is mainly expressed in immune cells, whereas CB1 receptor is the most abundant G protein-coupled receptor expressed in the brain. CB1 receptor is expr…
Dezinformācijas ierobežošana: Baltijas un Polijas valstu gadījumi
2022
Bakalaura darba tēma ir “Dezinformācijas ierobežošana: Baltijas un Polijas valstu gadījumi”. Darba ietvaros tika pētīta Krievijas izplatītās dezinformācijas ierobežošana nacionālā līmenī Baltijas valstīs un Polijā. Tā novērtēšanai tika izmantots Maikla Hovleta (Michael Howlett) rīcībpolitikas instrumentu konceptuālais ietvars, kas plašāk apskatīts darba teorētiskajā daļā. Pētījuma izstrādes laikā autore veica analīzi kvalitatīvajā pētījuma stratēģijā. Pētījuma rezultāti atklāj, ka tiek izmantoti visi rīcībpolitikas instrumenti neatkarīgi no dažādajām perspektīvām, kādas valstis pielieto dezinformatīva satura ierobežošanā laika posmā. Tika secināts, ka valstīs nepieciešams ieviest visaptvero…
Predictive Capacity of Boar Sperm Morphometry and Morphometric Sub-Populations on Reproductive Success after Artificial Insemination
2021
The aim of the study was to compare the morphometric features of sperm head sizeand shape from the Pietrain line and the Duroc×Pietrain boar crossbred terminal lines, and toevaluate their relationship with reproductive success after artificial insemination of sows producedfrom crossbreeding the York, Landrace and Pietrain breeds. Semen samples were collected from 11sexually mature boars. Only ejaculates with greater than 70% motility rate and <15% of abnormalsperm were used for artificial inseminations (AI) and included in the study. Samples were analyzedusing an ISAS®v1 computer-assisted sperm analysis system for eight morphometric parameters ofhead shape and size (CASA-Morph). Sub-populat…