Search results for "BPS"
showing 10 items of 28 documents
Extracellular Vesicles Shed by Melanoma Cells Contain a Modified Form of H1.0 Linker Histone and H1.0 mRNA-binding Proteins
2016
Extracellular vesicles (EVs) are shed in the extracellular environment by both prokaryotes and eukaryotes. Although produced from both normal and cancer cells, malignant cells release a much higher amount of EVs, which also contain tumor-specific proteins and RNAs. We previously found that G26/24 oligodendroglioma cells shed EVs that contain the pro-apoptotic factors FasL and TRAIL1-2. Interestingly, G26/24 release, via EVs, extracellular matrix remodelling proteases3, and H1° histone protein4, and mRNA. To shed further light on the role of EVs in discarding proteins and mRNAs otherwise able to counteract proliferative signals, we studied a melanoma cell line (A375). We found that also thes…
Extracellular Vesicle‐Associated RNA as a Carrier of Epigenetic Information
2017
Post-transcriptional regulation of messenger RNA (mRNA) metabolism and subcellular localization is of the utmost importance both during development and in cell differentiation. Besides carrying genetic information, mRNAs contain cis-acting signals (zip codes), usually present in their 5'- and 3'-untranslated regions (UTRs). By binding to these signals, trans-acting factors, such as RNA-binding proteins (RBPs), and/or non-coding RNAs (ncRNAs), control mRNA localization, translation and stability. RBPs can also form complexes with non-coding RNAs of different sizes. The release of extracellular vesicles (EVs) is a conserved process that allows both normal and cancer cells to horizontally tran…
Retinol oxidation to retinoic acid in human thyroid glandular cells.
2014
Abstract Retinoic acid is regarded as the retinol metabolite that controls proliferation and differentiation of epithelial cells. In the present study, we investigated the potential role of xanthine dehydrogenase (XDH) in retinoic acid biosynthesis in human thyroid glandular cells (HTGC). In particular, we observed that cellular retinoids binding proteins (CRBPs) are also implicated in the biosynthetic pathway leading to retinoic acid formation in primary cultures of HTGC, as we have already reported for human mammary epithelial cells (HMEC). After partial protein purification, the enzyme responsible for retinoic acid biosynthesis was identified and quantified as XDH by immunoassay, by its …
The Impact of COVID-19 Quarantine on Patients With Dementia and Family Caregivers: A Nation-Wide Survey
2021
IntroductionPrevious studies showed that quarantine for pandemic diseases is associated with several psychological and medical effects. The consequences of quarantine for COVID-19 pandemic in patients with dementia are unknown. We investigated the clinical changes in patients with Alzheimer’s disease and other dementias, and evaluated caregivers’ distress during COVID-19 quarantine.MethodsThe study involved 87 Italian Dementia Centers. Patients with Alzheimer’s Disease (AD), Dementia with Lewy Bodies (DLB), Frontotemporal Dementia (FTD), and Vascular Dementia (VD) were eligible for the study. Family caregivers of patients with dementia were interviewed by phone in April 2020, 45 days after …
Caring for People With Dementia Under COVID-19 Restrictions: A Pilot Study on Family Caregivers
2021
IntroductionThe present pilot study examined to what extent the COVID-19 lockdown affected the behavioral and psychological symptoms of dementia (BPSD) in people with dementia and worsened their family caregivers’ distress. The associations between changes in the BPSD of relatives with dementia (RwD) and in their caregivers’ distress, and sense of social and emotional loneliness, and resilience were also investigated.Materials and MethodsThirty-five caregivers of RwD attending formal healthcare services before the COVID-19 lockdown volunteered for the study, and were interviewed by phone during the lockdown. Caregivers completed the NeuroPsychiatric Inventory (NPI) to assess their care reci…
A review on optimization and cost-optimal methodologies in low-energy buildings design and environmental considerations
2019
Abstract The topic of low-energy buildings received a widespread and growing interest in last years, thanks to energy saving policies of developed countries. The design of a low-energy building is addressed with energy saving measures and renewable energy generation, but the correct assessment of phenomena occurring in a building usually requires to perform dynamic simulations and to analyze multiple scenarios to attain the optimal solution. The optimality of a technical solution may be subject to contrasting constraints and objectives. For this reason, designers may employ mathematical optimization techniques, a non-familiar topic to most of building designers. In this paper, a review on o…
Nonlinear dynamical model of Costas loop and an approach to the analysis of its stability in the large
2015
The analysis of the stability and numerical simulation of Costas loop circuits for highfrequency signals is a challenging task. The problem lies in the fact that it is necessary to simultaneously observe very fast time scale of the input signals and slow time scale of phase difference between the input signals. To overcome this difficult situation it is possible, following the approach presented in the classical works of Gardner and Viterbi, to construct a mathematical model of Costas loop, in which only slow time change of signal's phases and frequencies is considered. Such a construction, in turn, requires the computation of phase detector characteristic, depending on the waveforms of the…
Nonlinear dynamical model of Costas loop and an approach to the analysis of its stability in the large
2015
The analysis of the stability and numerical simulation of Costas loop circuits for high-frequency signals is a challenging task. The problem lies in the fact that it is necessary to simultaneously observe very fast time scale of the input signals and slow time scale of phase difference between the input signals. To overcome this difficult situation it is possible, following the approach presented in the classical works of Gardner and Viterbi, to construct a mathematical model of Costas loop, in which only slow time change of signal?s phases and frequencies is considered. Such a construction, in turn, requires the computation of phase detector characteristic, depending on the waveforms of th…
Identification of nuclear proteins which interact with H1° mRNA.
2012
In developing rat brain the synthesis of H1° histone is mainly regulated at posttranscriptional level and probably depends on RNA-binding proteins (RBPs) (1). We previously identified RBPs apparently specific for this messenger (2) and cloned two novel proteins by screening an expression cDNA library by binding to radiolabeled RNA (3-10). Here we report the use of biotinylated H1° RNA as bait to isolate by chromatography nuclear proteins which interact with H1° mRNA. We identified by mass spectrometry abundant RBPs and molecular chaperones. By western blot we also evidenced, among the RNA-bound proteins, the cold shock domain-containing protein 2 (CSD-C2, also know as PIPPin), a brain-enric…
Intersecting Defects and Supergroup Gauge Theory
2021
Journal of physics / A 54(43), 435401 (2021). doi:10.1088/1751-8121/ac2716