Search results for "BS"
showing 10 items of 20952 documents
Characterization of Purified Green Bell Pepper Hydroperoxide Lyase Expressed by Yarrowia Lipolytica: Radicals Detection during Catalysis
2007
International audience; The optimization of the expression of recombinant 6-His-tagged HPO lyase in Yarrowia lipolytica is described: 1800U/L of culture was detected at 24h of culture on a medium containing olive oil as the sole carbon source. The enzyme was purified by IMAC and showed an optimal pH at 5.5, an optimal temperature at 20^\circC and a Km value of 9μM with 13-HPOD substrate. The participation of radicals during the catalysis of purified bell pepper fruit hydroperoxide lyase has been observed by electron paramagnetic resonance spectroscopy and the yet unidentified radical species might be an alkyl or alkoxyl radical linked to the enzyme.
Effect of Enzyme Amounts Used in Gastrointestinal Digestion Upon Solubility and Caco-2 Cell Uptake Assays of Minerals from Infant Formulas
2005
The effect of enzyme amounts used in gastrointestinal in vitro digestion upon the solubility and Caco-2 cell uptake of calcium, iron and zinc from infant formulas (IFs) was studied. Different amounts of enzymes (g enzyme/g IF), pepsin (0.002 and 0.048), pancreatin (0.0005, 0.002 and 0.01) and bile extract (0.003, 0.125 and 0.0625) were assayed. Mineral soluble contents and mineral uptakes by Caco-2 cells were affected by the enzyme amounts used in digestion. Although the highest mineral solubility (Ca 98.6 vs 46.2%; Fe 98.1 vs 83.9%; Zn 98.4 vs 83%) was obtained when the lowest enzyme (pepsin 0.002 vs 0.048; pancreatin 0.0005 vs 0.01g/g IF) and bile extract (0.003 vs 0.0625g/g IF) amounts …
Genetic Structure of the Limecola Balthica Population in the Gulf of Riga, Baltic Sea
2020
Abstract Samples of Limecola balthica with normal and deformed shells were collected from ten sites throughout the Gulf of Riga. Genetic diversity was evaluated by the retrotransposon-based iPBS method. Samples had close mutual genetic distances, which showed that all of them belong to one wider population of the Gulf of Rīga. No direct relationship between the activity of retrotransposons and deformation of shells was found.
Effects of photoperiod on life-history and thermal stress resistance traits across populations of Drosophila subobscura
2019
Introduction Organisms use environmental cues to match their phenotype with the future availability of resources and environmental conditions. Changes in the magnitude and frequency of environmental cues such as photoperiod and temperature along latitudes can be used by organisms to predict seasonal changes. While the role of temperature variation on the induction of plastic and seasonal responses is well established, the importance of photoperiod for predicting seasonal changes is less explored. Materials and methods Here we studied changes in life‐history and thermal stress resistance traits in Drosophila subobscura in response to variation in photoperiod (6:18, 12:12 and 18:6 light:dark …
Mixed company : a framework for understanding the composition and organization of mixed‐species animal groups
2020
Mixed‐species animal groups (MSGs) are widely acknowledged to increase predator avoidance and foraging efficiency, among other benefits, and thereby increase participants' fitness. Diversity in MSG composition ranges from two to 70 species of very similar or completely different phenotypes. Yet consistency in organization is also observable in that one or a few species usually have disproportionate importance for MSG formation and/or maintenance. We propose a two‐dimensional framework for understanding this diversity and consistency, concentrating on the types of interactions possible between two individuals, usually of different species. One axis represents the similarity of benefit types …
Were climatic changes a driving force in hominid evolution?
2000
International audience; A comparison of externalist and internalist approaches in hominid evolution shows that the externalist approach, with its claim that climate was responsible for the appearance of bipedalism and hominization, now seems to be ruled out by the biological, palaeogeographical, palaeontological and palaeoclimatic data on which it was based. Biological data support the embryonic origin of cranio-facial contraction, which determined the increase in cranial capacity and the shift in the position of the foramen magnum implying bipedalism. In the internalist approach, developmental biology appears as the driving force of hominid evolution, although climate exerts a significant …
Lake restoration influences nutritional quality of algae and consequently Daphnia biomass
2020
AbstractFood quality is one of the key factors influencing zooplankton population dynamics. Eutrophication drives phytoplankton communities toward the dominance of cyanobacteria, which means a decrease in the availability of sterols and long-chain polyunsaturated fatty acids (EPA and DHA). The effects of different restoration measures on the nutritional quality of the phytoplankton community and subsequent impacts on zooplankton biomass have rarely been considered. We analyzed the nutritional quality of phytoplankton in the eutrophic Lake Vesijärvi in southern Finland over a 37-year period, and studied the impacts of two restoration measures, biomanipulation and hypolimnetic aeration, on th…
Physiological Responses of Wetland Species Rumex Hydrolapathum to Increased Concentration of Biogenous Heavy Metals Zn and Mn in Substrate
2020
Abstract The aim of the present study was to determine if individuals of Rumex hydrolapathum Huds native to saline wetlands are able to tolerate high concentration of biogenous heavy metals Zn and Mn in substrate and to accumulate high concentration of these metals in aboveground parts. Plant physiological status was monitored by using non-destructive analysis of chlorophyll and chlorophyll a fluorescence. R. hydrolapathum plants accumulated up to 1840 mg·kg−1 Zn and 6400 mg·kg−1 Mn in older leaves. The usefulness of monitoring changes in chlorophyll concentration and chlorophyll a fluorescence parameters to predict physiological response of R. hydrolapathum plants to excess Zn and Mn was n…
Relationship Between Functional Traits, Functional Types, and Habitat in Boreonemoral Bryophytes
2020
Abstract The aim of the present study was to determine a relationship between physiological traits and functional types of bryophytes from five boreonemoral habitats with a particular emphasis on discriminative ability of these traits. Sampling of 25 species was performed four times during one season. Water content, chlorophyll a fluorescence and photosynthetic pigment concentration were measured in field and water-equilibrated samples. Principal component analysis indicated the existence of an inverse relationship between concentration of pigments and water content. Linear discriminant analysis showed that relatively high mean predicted posterior probabilities of correct classification of …
OMICfpp: a fuzzy approach for paired RNA-Seq counts
2019
© The Author(s) 2019.