Search results for "BT"

Regulation of stress response in Oenococcus oeni as a function of environmental changes and growth phase

2000

International audience; Oenococcus oeni is a lactic acid bacterium which is able to grow in wine and perform malolactic fermentation. To survive and grow in such a harsh environment as wine, O. oeni uses several mechanisms of resistance including stress protein synthesis. The molecular characterisation of three stress genes hsp18, clpX, trxA encoding for a small heat shock protein, an ATPase regulation component of ClpP protease and a thioredoxin, respectively, allow us to suggest the existence in O. oeni of multiple regulation mechanisms as is the case in Bacillus subtilis. One common feature of these genes is that they are expressed under the control of housekeeping promoters. The express…

A Trans-amplifying RNA Vaccine Strategy for Induction of Potent Protective Immunity

2019

Here, we present a potent RNA vaccine approach based on a novel bipartite vector system using trans-amplifying RNA (taRNA). The vector cassette encoding the vaccine antigen originates from an alphaviral self-amplifying RNA (saRNA), from which the replicase was deleted to form a transreplicon. Replicase activity is provided in trans by a second molecule, either by a standard saRNA or an optimized non-replicating mRNA (nrRNA). The latter delivered 10- to 100-fold higher transreplicon expression than the former. Moreover, expression driven by the nrRNA-encoded replicase in the taRNA system was as efficient as in a conventional monopartite saRNA system. We show that the superiority of nrRNA- ov…

Efficacy and quality of life after 10 years of transobturator tape surgery for stress urinary incontinence

2015

Development of an Efficient In Vivo System (P-junc-TpaseIS(1223)) for Random Transposon Mutagenesis of Lactobacillus casei

2012

ABSTRACT The random transposon mutagenesis system P junc -TpaseIS 1223 is composed of plasmids pVI129, expressing IS 1223 transposase, and pVI110, a suicide transposon plasmid carrying the P junc sequence, the substrate of the IS 1223 transposase. This system is particularly efficient in Lactobacillus casei , as more than 10,000 stable, random mutants were routinely obtained via electroporation.

Soil erosion assessment on tillage and alternative soil managements in a Sicilian vineyard

2011

Mediterranean crops favour high erosion rates. Vineyards use to reach the highest soil and water losses due to the lack of vegetation cover. A topographical approach by means of the use of vineyards poles as fixed reference point as erosion markers allowed to quantify high and non-sustainable soil erosion rates on the Sicilian vineyards during 9 years. In order to develop strategies to control the soil losses, seven land management were selected and applied in a typical blanc wine grape vineyard located in southwestern Sicily. Comparable plots were managed traditionally using conventional tillage and alternatively using various cover crops: 1) Vicia faba; 2) Vicia faba and Vicia sativa; 3) …

Capturing colorectal cancer inter-tumor heterogeneity in patient-derived xenograft (PDX) models

2019

Patient‐derived xenograft (PDX) models have become an important asset in translational cancer research. However, to provide a robust preclinical platform, PDXs need to accommodate the tumor heterogeneity that is observed in patients. Colorectal cancer (CRC) can be stratified into four consensus molecular subtypes (CMS) with distinct biological and clinical features. Surprisingly, using a set of CRC patients, we revealed the partial representation of tumor heterogeneity in PDX models. The epithelial subtypes, the largest subgroups of CRC subtype, were very ineffective in establishing PDXs, indicating the need for further optimization to develop an effective personalized therapeutic approach …

The role of the economic crisis on the competitiveness of the agri-food sector in the main Mediterranean countries

2014

Th e world economic crisis that, since 2008 has also struck the real economy, cannot be attributed only to the United States bubble which in 2007 involved the mortgage credit market, but it is the result of a series of factors among which the imbalance of the fi nancial market, of the public accounts of the main economies and the real sector. Also agricul- ture, which has always been considered an anti-cyclic sector, has seen a strong slowdown with a plunge in the trade fl ows. Th is paper analyses the changes which happened to the competitive position in the world market of some Mediterranean countries and of France, Italy, Spain and Turkey in particular trying, moreover, to understand the…

Schlüssel zum systematischen Catalog der Dorpater Universitäts-Bibliothek [Schlüssel zum systematischen Catalog der Dorpater Universitätsbibliothek]

1873

El subtitulado en tanto que modalidad de traducción fílmica dentro del marco teórico de los Estudios sobre Traducción. (Misterioso asesinato en Manha…

1997

Esta tesis tiene como finalidad rescatar al fenómeno traductor del ostracismo, para ello enfoca la realidad subtituladora desde dos ángulos, su posicionamiento cultural en el polisistema español y analizar los datos empíricos que se materializan en MLM.

La traducción audiovisual en España e Hispanoamérica: aspectos históricos y estudio de caso sobre técnicas de traducción

2023

La presente tesis doctoral pretende profundizar en una serie de cuestiones que, en nuestra opinión, resultan de interés en el ámbito de los estudios en traducción audiovisual. Por un lado, se presenta un estudio histórico y de archivo acerca de la traducción audiovisual que presta especial atención al desarrollo de esta modalidad general de traducción desde los años del cine mudo hasta la llegada del cine sonoro y a las razones detrás de la adopción del doblaje y el subtitulado en España e Hispanoamérica mediante la lectura de bibliografía especializada y la lectura y análisis de fuentes primarias, así como al origen y las características del español neutro y el español europeo en el ámbito…