6533b861fe1ef96bd12c4fd5
RESEARCH PRODUCT
Development of an Efficient In Vivo System (P-junc-TpaseIS(1223)) for Random Transposon Mutagenesis of Lactobacillus casei
Hélène LicandroHélène Licandro-serautPascale SerrorPascale SerrorSophie BrinsterSophie BrinsterJean-françois CavinEmmanuelle MaguinEmmanuelle MaguinMaarten Van De GuchteMaarten Van De GuchteHélène ScornecPhilippe J. Sansonettisubject
Transposable element[SDV.SA]Life Sciences [q-bio]/Agricultural sciencesTn3 transposonLactobacillus casei[SDV]Life Sciences [q-bio]TransposasesVECTORGenetics and Molecular BiologyDELBRUECKII SUBSP BULGARICUSApplied Microbiology and BiotechnologyBACILLUS-SUBTILIS03 medical and health sciencesPlasmidEscherichia coliSTREPTOCOCCUS[ SDV.SA ] Life Sciences [q-bio]/Agricultural sciencesTransposaseDNA Primers030304 developmental biologyGenetics0303 health sciencesEcologybiologyRandom030306 microbiologyINSERTION SEQUENCESElectroporationbiology.organism_classificationSleeping Beauty transposon systemMolecular biologyGENETRANSFORMATIONGROUP-BBlotting SouthernLacticaseibacillus caseiLactobacillusMutagenesisDNA Transposable ElementsbacteriaTransposon mutagenesisELECTROPORATIONPLASMIDPlasmidsFood ScienceBiotechnologydescription
ABSTRACT The random transposon mutagenesis system P junc -TpaseIS 1223 is composed of plasmids pVI129, expressing IS 1223 transposase, and pVI110, a suicide transposon plasmid carrying the P junc sequence, the substrate of the IS 1223 transposase. This system is particularly efficient in Lactobacillus casei , as more than 10,000 stable, random mutants were routinely obtained via electroporation.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2012-01-01 |