Search results for "Bacterium"

Exiguobacterium pavilionensis str. N139, a halotolerant, UV-B and metal resistant bacteria from a high-altitude Andean lake

2016

We report the genome sequence of Exiguobacterium pavilionensis str. N139, isolated from a high-altitude Andean lake. The 2,952,588-bp genome contains one chromosome and three megaplasmids. The genome analysis suggests the presence of enzymes that confer E. pavilionensis str. N139 the ability to grow under multiple environmental extreme conditions, including high concentrations of different metals and high ultraviolet B radiation. Moreover, the regulation of its tryptophan biosynthesis suggests that novel pathways remain to be discovered, and that these pathways might be fundamental in the amino acid metabolism of the microbial community from Laguna Negra, Argentina .

Relationship between the endangered freshwater pearl mussel Margaritifera margaritifera, its salmonid host and co-infectants

2018

The relationship between the freshwater pearl mussel (hereafter FPM), Margaritifera margaritifera, and its salmonid host — pivotal for the conservation of this endangered bivalve — is characterized by a long parasitic stage (up to 11 months) and excessive host specificity. This thesis focuses on this relationship by experimentally studying the effect of FPM infection on the growth and resistance of salmonid fish and how the infection influences vulnerability of the host to other parasites and diseases, as well as how exposure of the host to other parasites affects vulnerability to FPM. Infection with FPM results in (i) reduced growth of the host, brown trout, during the parasitic period. Gl…

The cockroach Blattella germanica obtains nitrogen from uric acid through a metabolic pathway shared with its bacterial endosymbiont.

2014

Uric acid storedin the fat bodyof cockroaches is a nitrogen reservoirmobilized in times of scarcity. The discovery of urease in Blattabacterium cuenoti, the primary endosymbiont of cockroaches, suggests that the endosymbiont may participate in cockroach nitrogen economy. However, bacterial urease may only be one piece in the entire nitrogen recycling process from insect uric acid. Thus, in addition to the uricolytic pathway to urea, there must be glutamine synthetase assimilating the released ammonia by the urease reaction to enable the stored nitrogen to be metabolically usable. None of the Blattabacterium genomes sequenced to date possess genes encoding for those enzymes. To test the host…

Immunostimulants in prevention of columnaris disease of rainbow trout, Oncorhynchus mykiss (Walbaum).

2009

Chondroitin AC lyase activity is related to virulence of fish pathogenic Flavobacterium columnare.

2006

The virulence of eight Flavobacterium columnare strains was studied to find correlations between several virulence-related factors and virulence. Virulence was tested in vivo using rainbow trout, Oncorhynchus mykiss (Walbaum). Suggested virulence-related factors such as production of the degradative enzyme chondroitin lyase, plasmid occurrence and adhesion capability were studied in vitro. Infection with the four most virulent strains resulted in 95-100% mortality within 114 h. Chondroitin lyase activity was found to be significantly related to the virulence of the strains at 25 degrees C and it was also shown to be temperature-dependent, being higher at 25 degrees C than at 20 degrees C. V…

Carriage of potentially fish-pathogenic bacteria in Sparus aurata cultured in Mediterranean fish farms.

2003

A bacteriological survey of gilthead sea bream Sparus aurata from different fish farms and culture systems on the Spanish Mediterranean coast was conducted. Three different studies were performed. Study A included hatchery-reared larvae; Study B, periodic examination of randomly sampled growing fish; and Study C, growing fish sampled only during mortality/morbidity events. In Studies B and C, sea cages, earth ponds and indoor tanks were surveyed, and in both cases diseased (showing clinical signs) and non-diseased fish were included. In Study A, a shift from Vibrio spp. (30 d after hatching) to oxidative species (60 d after hatching) was detected, and no mortality events were registered. Th…

Influence of rearing conditions on Flavobacterium columnare infection of rainbow trout, Oncorhynchus mykiss (Walbaum).

2005

The influence of rearing conditions on Flavobacterium columnare infection of rainbow trout, Oncorhynchus mykiss (Walbaum), was studied experimentally in the laboratory and at a fish farm. In experiment I, the effect of parasitic infection on columnaris disease was studied using F. columnare carrier fish. The fish were exposed to Diplostomum spathaceum cercariae and a set of other stressors in order to induce clinical columnaris infection. Parasitic infection and other stressors failed to induce the disease. Disease occurred when the fish were challenged with F. columnare, but D. spathaceum infection did not enhance the severity of the infection. In experiment II, the influence of rearing de…

Environment may be the source of Flavobacterium columnare outbreaks at fish farms

2012

Summary Flavobacterium columnare, causing columnaris disease, was isolated for the first time from free water and biofilms in the environment outside fish farms. Fourteen isolates were found from Central Finland from a river by a water intake of a salmonid farm and 400 m upstream of the farm. One isolate was from a lake not under the influence of any fish farming. The bacterium could not be isolated from five other lakes in Central Finland or from three lakes in Eastern Finland, none of them in use for fish farming. Among the environmental isolates there was both genetic variability and difference in virulence, but the isolates were less virulent than the isolates originating from a disease…

The development of species-specific probes for a quantitative estimation of non-cultured bacteria in groundwater samples

2012

The aim of this study was to quantify different bacteria in drinking water production wells that supply the city of Mainz (capital of Rhineland-Palatinate, Germany). Knowledge of the counts of individual microorganisms is important for the appraisal of the hygienic and sensory quality of drinking water. Specific 16S rRNA probes were designed for 14 previously identified but non-cultured bacterial species ( Ferribacterium limneticum , Hydrogenophaga sp., Methylotenera sp., Janthinobacterium sp., Pseudomonas sp., Rhizobium selenireducens , Rhodoferax sp., Nitrosomonadaceae bacterium, Thauera sp. and five strains which could not be identified to species level). With fluorescence in situ hybrid…

Numerical Taxonomy of Vibrionaceae Isolated from Cultured Amberjack ( Seriola dumerili ) and Surrounding Water

2003

A numerical taxonomic study was performed on 148 isolates of Gram-negative, heterotrophic, facultative anaerobic bacteria isolated from amberjack (Seriola dumerili) and its surrounding culture water. The study included 30 type and reference strains belonging to genera Vibrio, Listonella, and Photobacterium. The strains were characterized by 109 morphological, biochemical, physiological, and nutritional tests. Cluster analysis of similarity matrices obtained with S(SM) and S(J) coefficients was carried out. UPGMA (unweighted pair group mathematical average) analysis defined 11 phena at S(SM) values > or = 86%. Nine phena were identified as Vibrio alginolyticus, V. fischeri, V. harveyi, V. ca…