Search results for "Bacterium"

Data from: Key roles for freshwater Actinobacteria revealed by deep metagenomic sequencing

2014

Freshwaters ecosystems are critical but fragile environments directly affecting society and its welfare. However, our understanding of genuinely freshwater microbial communities, constrained by our capacity to manipulate its prokaryotic participants in axenic cultures, remains very rudimentary. Even the most abundant components, freshwater Actinobacteria, remain largely unknown. Here, applying deep metagenomic sequencing to the microbial community of a freshwater reservoir, we were able to circumvent this traditional bottleneck and reconstruct de novo seven distinct streamlined actinobacterial genomes. These genomes represent three new groups of photoheterotrophic, planktonic Actinobacteria…

Research data of an article: "Application of high resolution melting assay (HMR) to study temperature dependent infraspecific competition in an patho…

2017

Studies on species’ responses to climate change have focused largely on the direct effect of abiotic factors and in particular temperature, neglecting the effects of biotic interactions in determining the outcome of climate change projections. Many microbes rely on strong interference competition; hence the fitness of many pathogenic bacteria could be a function of both their growth properties and intraspecific competition. However, due to technical challenges in distinguishing and tracking individual strains, experimental evidence on intraspecific competition has been limited so far. Here, we developed a robust application of the high-resolution melting (HRM) assay to study head-to-head co…

Detection of Mycobacterium tuberculosis complex in cattle by PCR on milk, lymphnode and nasal swabs.

1998

The PCR technique was applied to the diagnosis of tuberculosis in live cattle, and both skin-test-negative and skin-test-positive animals were studied. DNA was taken from various sources including specimens of lymph node aspirates, milk, and nasal swabs. After slaughter and visual inspection, tissues such as lymph nodes, lungs, and udders from tuberculin reactors were tested by the same technique. Specific oligonucleotide primers internal to the IS6110 insertion element were used to amplify a 580-bp fragment. A 182-bp fragment was obtained by designating a nested PCR from the first amplification product. This fragment was cloned and sequenced, and after being labeled it was employed in dot …

Mycobacterium tuberculosis multirezistences noteikšana

2018

Latvijā un pasaulē joprojām mirst no ārstējamas slimības – tuberkuloze ir devītais nāves cēlonis pasaulē. Nozīmīga sabiedrības veselības problēma ir M. tuberculosis multirezistence pret antibakteriālajiem līdzekļiem. Rezistence pret antibakteriālajiem līdzekļiem galvenokārt saistīta ar to, ka daļa tuberkulozes slimnieku netiek savlaicīgi atklāti, tādējādi turpinot slimības aktīvu transmisiju sabiedrībā; antibakteriālo līdzekļu nepareizu pielietošanu – priekšlaicīgi pārtrauc terapiju vai lieto zāles neregulāri, kā rezultātā rodas multirezistentās tuberkulozes formas. Darba mērķis: Noteikt no klīniskā materiāla izdalīto M. tuberculosis celmu sastopamības biežumu un to rezistenci pret antibakt…

Bifidobacterium pseudocatenulatum CECT 7765 supplementation restores altered vascular function in an experimental model of obese mice

2017

Aims. Bifidobacterium pseudocatenulatum CECT 7765 improves metabolic and immunological altered functions in high fat fed mice, however little is known about the effects of potential probiotics on vascular reactivity. The aim of the present study was to investigate the effects of a potential probiotic strain, Bifidobacterium pseudocatenulatum CECT 7765, on vascular response in obese mice. Methods. Aorta samples were obtained from mice, which were divided into three groups: a control group, receiving a standard diet; an obese group, receiving a high-fat diet; and an obese group receiving high-fat diet and a daily dose of B. pseudocatenulatum CECT 7765 by oral gavage. Aortic rings were suspend…

Antibacterial Efficiency of Surface-Immobilized Flavobacterium-Infecting Bacteriophage

2019

Control of bacterial diseases by bacteriophages (phages) is gaining more interest due to increasing antibiotic resistance. This has led to technologies to attach phages on surfaces to form a biomaterial that can functionally display phages that interact with bacteria, to carry out successful infection cycles. Such a material could be applied in many environments, where the target pathogens are expected. Although this approach has been applied successfully in a few studies already, the basis of the antibacterial effect by the immobilized phages is unclear, and the interpretation of the results depends on the study. Here, we studied the phage attachment density, their detachment rate and infe…

Exploring phage-bacterium interactions : new ways to combat a fish pathogen

2014

Sex and sexual ornamentation associated with survival of the cyprinid fish, Rutilus rutilus, under disease stress

2013

By conveying information of disease resistance, sexual signals may be used as cues for adaptive mate choice. Here we report observations on survival of laboratory-maintained, wild-collected, sexually mature, ready-to-spawn cyprinid fish, Rutilus rutilus (roach), under accidental epidemic attributed to Flavobacterium psychrophilum, diagnosed using species-specific PCR. The fish were maintained in a single tank. During the 27 days observation period, both the proportion of fish surviving the infection as well as the length-adjusted mean survival time of the fish that died was the highest among the high-ornamented males with large breeding tubercles, intermediary among the low-ornamented males…

Latgales reģionā cirkulējošo Mycobacterium tuberculosis genotipu raksturojums

2019

Tuberkuloze ir pasaulē izplatīta infekcijas slimība, kuras izraisītājas ir M. tuberculosis kompleksā ietilpstošās mikobaktērijas. Darbs tika veikts, lai noteiktu un raksturotu Latgales reģionā cirkulējošo M. tuberculosis genotipus. Darbā ir izmantotas molekulāras genotipēšanas metodes: starpfragmentu oligonukleotīdu genotipēšanas metode, kas ir balstīta uz polimerāzes ķēdes reakciju un tiešā atkārtojuma (DR) reģiona amplificēšanu; restrikcijas fragmentu garuma polimorfisma analīzes metode, kur M. tuberculosis genotips tiek noteikts pēc insercijas sekvenču IS6110 kopiju skaita un izvietojuma atšķirībām hromosomā. Rezultātos tiek atspoguļots, kādas līnijas un apakšlīnijas atrastas Latgales re…

Starvation can diversify the population structure and virulence strategies of an environmentally transmitting fish pathogen

2014

Background. Generalist bacterial pathogens, with the ability for environmental survival and growth, often face variable conditions during their outside-host period. Abiotic factors (such as nutrient deprivation) act as selection pressures for bacterial characteristics, but their effect on virulence is not entirely understood. “Sit and wait” hypothesis expects that long outside-host survival selects for increased virulence, but maintaining virulence in the absence of hosts is generally expected to be costly if active investments are needed. We analysed how long term starvation influences bacterial population structure and virulence of an environmentally transmitting fish pathogen Flavobacter…