Search results for "Bacteroides"
showing 3 items of 23 documents
Deoxycholic Acid Methyl Ester — a Novel Bacterial Metabolite of Cholic Acid
1985
Summary Methyl deoxycholate was identified as a novel bacterial metabolite of cholate, produced anaerobically by strains of most saccharolytic Bacteroides species ( B. distasonis, B. eggerthii, B. fragilis, B. incommunis, B. thetaiotaomicron, B. uniformis, B. variabilis, B. vulgatus , and unnamed species). It was also detected with a few strains of Eubacterium and Lactobacillus species. Among 2476 freshly isolated human fecal cultures the frequency of carboxyl group esterification was comparable with that of 7α-dehydroxylation (92 versus 102 cultures). Both activities were, however, lost for unknown reasons after serial transfers. Two of ten mixed fecal cultures tested esterified 3α,7α- or …
Metagenomic Analysis of Milk of Healthy and Mastitis-Suffering Women.
2015
Background: Some studies have been conducted to assess the composition of the bacterial communities inhabiting human milk, but they did not evaluate the presence of other microorganisms, such as fungi, archaea, protozoa, or viruses. Objective: This study aimed to compare the metagenome of human milk samples provided by healthy and mastitis-suffering women. Methods: DNA was isolated from human milk samples collected from 10 healthy women and 10 women with symptoms of lactational mastitis. Shotgun libraries from total extracted DNA were constructed and the libraries were sequenced by 454 pyrosequencing. Results: The amount of human DNA sequences was ≥ 90% in all the samples. Among the bacteri…
T cell specificity and cross reactivity towards enterobacteria,Bacteroides,Bifidobacterium, and antigens from resident intestinal flora in humans
1999
BACKGROUNDT cell responses to normal intestinal bacteria or their products may be important in the immunopathogenesis of chronic enterocolitis.AIMSTo investigate the T cell specificity and cross reactivity towards intestinal bacteria.PATIENTS/METHODST cell clones were isolated with phytohaemagglutinin from peripheral blood and biopsy specimens of inflamed and non-inflamed colon from five patients with inflammatory bowel disease (IBD) and two controls. T cell clones were restimulated with anaerobicBacteroides andBifidobacteria species, enterobacteria, and direct isolates of aerobic intestinal flora. T cell phenotype was analysed by single-cell immunocyte assay.RESULTSAnalysis of 96 T cell cl…