Search results for "Biochemical"
showing 10 items of 870 documents
Safety Assessment and Migration Tests
2018
This chapter outlines (i) the advances to reduce the uncertainty in nanomaterials (NMs) determination; (ii) the results from migration experiments conducted with nano-clay, nanosilver, and other nanomaterial-containing polymers; and (iii) the different established protocols for risk assessment that ensure consumer's safety. The text starts highlighting the need of an understanding of potential exposure via migration into food and a deeper knowledge of the interaction of NMs with food constituents and their effects upon ingestion. The chapter covers the ability of the analytical techniques utilized to detect nanoparticles per se in food simulants. In addition, the results of the application …
Camellia japonica : A phytochemical perspective and current applications facing its industrial exploitation
2022
In response to the increased popularity of medicinal plants, a number of conservation groups are recommending the investigation on poorly characterized and widely distributed species, as it is the case of camellias. In particular, Camellia japonica L. is a widespread species found in Galicia (NW Spain), where it has been largely exploited with ornamental purposes. Recent findings on its phytochemical characterization showed thousands of bioactive ingredients, mostly represented by phenolic compounds, together with terpenoids, and fatty acids. These molecules present associated biological activities, acting as antioxidant, antimicrobial, anti-inflammatory, and anticancer agents. This review …
Applications of fast field cycling NMR relaxometry
2021
Abstract Fast field cycling (FFC) NMR relaxometry is emerging as a powerful tool to investigate physical chemistry properties of many systems in a number of different scientific fields. As an example, it is used to investigate environmental issues such as soil erosion, water, and nutrient dynamics in environmentally relevant porous systems, to discriminate among different kinds of foodstuff in order to understand possible source of adulteration and fraud, to evaluate the properties of new materials, and much more. In the present study, an overview about the possible applications of FFC NMR relaxometry is given. The paper is not intended to be exhaustive. Rather, it is thought to provide an …
Chromatographic fingerprint of the volatile fraction of rare Hedera helix honey and biomarkers identification
2018
Hedera helix (Irish ivy) honey is a very rare food product used in Ireland. The composition of the volatile fraction of this unique honey was studied for the first time using three different extraction procedures (SPE, USE and HS–SPME) and analyzed with gas chromatography and mass spectrometry. As expected, the use of combined techniques resulted in the identification of the detailed composition of honey volatiles. Identified constitutes belongs to the different chemical classes. The presence of 4(1H)-quinolinone, myrtenal and phenylacetonitrile was proposed as biomarkers of the botanical origin of ivy honey. Moreover, they are not widely widespread among honeys of different botanical origi…
PHENOLOGICAL ADAPTATION OF ANT-DISPERSED PLANTS TO SEASONAL VARIATION IN ANT ACTIVITY
2002
We studied a temperate plant community to examine whether the reproductive phenology of ant-dispersed plants is correlated with seasonal variation in seed dispersal activity of ants. We documented flowering and fruiting peak of 24 ant-dispersed and 251 non-ant-dispersed plant species. To characterize the activity of ants, we quantified the seasonal removal rates of greater celandine (Chelidonium majus) seeds. Ant-dispersed plants flowered on average 5.6 wk and fruited 7.1 wk earlier than those with other dispersal modes. This difference was not caused by variation in growth form or habitat. Mean fruiting peak of ant-dispersed plants was early July. Ant activity was especially high between M…
Computing Metal-Binding Proteins for Therapeutic Benefit
2021
Over one third of biomolecules rely on metal ions to exert their cellular functions. Metal ions can play a structural role by stabilizing the structure of biomolecules, a functional role by promoting a wide variety of biochemical reactions, and a regulatory role by acting as messengers upon binding to proteins regulating cellular metal-homeostasis. These diverse roles in biology ascribe critical implications to metal-binding proteins in the onset of many diseases. Hence, it is of utmost importance to exhaustively unlock the different mechanistic facets of metal-binding proteins and to harness this knowledge to rationally devise novel therapeutic strategies to prevent or cure pathological st…
Synthesis and Pharmacological Evaluation of [11C]4-Methoxy-N-[2-(thiophen-2-yl)imidazo[1,2-a]pyridin-3-yl]benzamide as a Brain Penetrant PET Ligand S…
2019
The α4/6βδ-containing GABAA receptors are involved in a number of brain diseases. Despite the potential of a δ-selective imaging agent, no PET radioligand is currently available for in vivo imaging...
Analytical procedures for short chain chlorinated paraffins determination - How to make them greener?
2019
Abstract The aim of the following paper was to gather current scientific information about the analytical protocols dedicated to measuring the content level of short-chain chlorinated paraffins (SCCPs) in various types of environmental samples. Moreover, the data about the basic validation parameters of applied procedures for SCCPs determination are listed. The main issue which is highlighted in the paper is the possibility of the application of green analytical chemistry (GAC) principals in the SCCPs measuring process to reduce the environmental impact of the applied methodology. Analytical methods dedicated to SCCPs determination contain a significant number of steps and require advanced …
Bacteriophage GIL01 gp7 interacts with host LexA repressor to enhance DNA binding and inhibit RecA-mediated auto-cleavage
2015
The SOS response in Eubacteria is a global response to DNA damage and its activation is increasingly associated with the movement of mobile genetic elements. The temperate phage GIL01 is induced into lytic growth using the host's SOS response to genomic stress. LexA, the SOS transcription factor, represses bacteriophage transcription by binding to a set of SOS boxes in the lysogenic promoter P1. However, LexA is unable to efficiently repress GIL01 transcription unless the small phage-encoded protein gp7 is also present. We found that gp7 forms a stable complex with LexA that enhances LexA binding to phage and cellular SOS sites and interferes with RecA-mediated auto-cleavage of LexA, the ke…
Phage-borne factors and host LexA regulate the lytic switch in phage GIL01.
2011
ABSTRACT The Bacillus thuringiensis temperate phage GIL01 does not integrate into the host chromosome but exists stably as an independent linear replicon within the cell. Similar to that of the lambdoid prophages, the lytic cycle of GIL01 is induced as part of the cellular SOS response to DNA damage. However, no CI-like maintenance repressor has been detected in the phage genome, suggesting that GIL01 uses a novel mechanism to maintain lysogeny. To gain insights into the GIL01 regulatory circuit, we isolated and characterized a set of 17 clear plaque ( cp ) mutants that are unable to lysogenize. Two phage-encoded proteins, gp1 and gp7, are required for stable lysogen formation. Analysis of …