Search results for "Bioengineering"

Evaluation of the use of phase-specific gene promoters for the expression of enological enzymes in an industrial wine yeast strain

1996

Genes as POT1, HSP104 and SSA3, which are late expressed in laboratory culture conditions are expressed only during the first few days in microvinifications in wine yeast cells. This effect is probably due to the different growth conditions and leads to useless levels of enzyme activity for a reporter gene. However the ACT1 promoter, which is constitutively expressed in laboratory conditions, produces sufficient amounts of enzyme activity in late fermentation phases.

Improving baculovirus transduction of mammalian cells by surface display of a RGD-motif

2006

An RGD-containing peptide, comprising 23 amino acids from the foot-and-mouth disease virus (FMDV) VP1 protein was engineered into the envelope of Autographa californica nuclear polyhedrosis virus surface (AcNPV) using two different display strategies. The RGD-motif is a well-described tripeptide, that by binding to cell surface integrins facilitates virus entry into cells. This epitope was displayed, either by directly modifying the native major envelope protein gp64 of AcNPV, or by incorporating a second, modified version of gp64 onto the virus surface. Transduction efficiencies of four mammalian cell lines were compared by detecting the expression of the reporter gene green fluorescent pr…

Three-Dimensional Superconducting Nanohelices Grown by He+-Focused-Ion-Beam Direct Writing

2019

Novel schemes based on the design of complex three-dimensional (3D) nanoscale architectures are required for the development of the next generation of advanced electronic components. He+ focused-ion-beam (FIB) microscopy in combination with a precursor gas allows one to fabricate 3D nanostructures with an extreme resolution and a considerably higher aspect ratio than FIB-based methods, such as Ga+ FIB-induced deposition, or other additive manufacturing technologies. In this work, we report the fabrication of 3D tungsten carbide nanohelices with on-demand geometries via controlling key deposition parameters. Our results show the smallest and highest-densely packed nanohelix ever fabricated s…

In-vitro evaluation of the shear bond strength and fluoride release of a new bioactive dental composite material

2022

Background The aim of this study was (1) to determine and compare the shear bond strength (SBS) of a bioactive composite “Activa Bioactive Restorative” with and without bonding agent and a nanocomposite “Filtek Z350 XT/Z350” and (2) to measure and compare the amount of fluoride release from a bioactive composite “Activa Bioactive Restorative” and a glass ionomer ”Equia forte”. Material and Methods Forty two dentin surfaces from freshly extracted human molars were prepared for shear bond strength testing. The specimens were randomly divided into three equal groups. The restorative materials were applied to all dentin surfaces according to the manufacturer’s instructions, using a special jig …

Cytotoxicity of glass ionomer cements containing silver nanoparticles

2015

Background: Some studies have investigated the possibility of incorporating silver nanoparticles (NAg) into dental materials to improve their antibacterial properties. However, the potential toxic effect of this material on pulp cells should be investigated in order to avoid additional damage to the pulp tissue. This study evaluated the cytotoxicity of conventional and resin-modified glass ionomer cements (GIC) with and without addition of NAg. Material and Methods: NAg were added to the materials at two different concentrations by weight: 0.1% and 0.2%. Specimens with standardized dimensions were prepared, immersed in 400 μL of culture medium and incubated at 37°C and 5% CO2 for 48 h to pr…

Field-evolved resistance to Bt toxins

2008

Molecular cloning of the RPS0 gene from Candida tropicalis.

2001

The Saccharomyces cerevisiae RPS0 A and B genes encode proteins essential for maturation of the 40S ribosomal subunit precursors. We have isolated a homologue of the RPS0 gene from Candida tropicalis, which we named CtRPS0. The C. tropicalisRPS0 encodes a protein of 261 amino acid residues with a predicted molecular weight of 28.65 kDa and an isoelectric point of 4.79. CtRps0p displays significant amino acid sequence homology with Rps0p from C. albicans, S. cerevisiae, Neurospora crassa, Schizosaccharomyces pombe, Pneumocystis carinii and higher organisms, such as human, mouse and rat. CtRPS0 on a high copy number vector can complement the lethal phenotype linked to the disruption of both R…

Assessing supply chain risks in the automotive industry through a modified MCDM-based FMECA

2020

Supply chains are complex networks that receive assiduous attention in the literature. Like any complex network, a supply chain is subject to a wide variety of risks that can result in significant economic losses and negative impacts in terms of image and prestige for companies. In circumstances of aggressive competition among companies, effective management of supply chain risks (SCRs) is crucial, and is currently a very active field of research. Failure Mode, Effects and Criticality Analysis (FMECA) has been recently extended to SCR identification and prioritization, aiming at reducing potential losses caused by lack of risk control. This article has a twofold objective. First, SCR assess…

Self-configuring multipath intra-mesh infrastructure QoS based routing

2016

International audience; Multi-path routing concept was largely exploited in wireless networks to provide benefits such as fault tolerance, load balancing, performance improvement in terms of latency, etc. In this paper, we propose a multi-path routing based protocol named MP-IMRR (Multi-path Intra-Mesh infrastructure Routing protocol) to improve our previously defined QoS based routing protocol for wireless mesh networks (i.e. IMRR). MP-IMRR is defined in order to achieve better reactivity and faster recovery from eventual route failures than the IMRR protocol by adopting the backup routes concept. Besides, given the complexity increase while considering network management systems, we adopt…

Run duration effects on the hydrodynamic properties of a loam soil estimated by steady-state infiltration methods

2020

Steady-state methods for the analysis of single-ring infiltration data are commonly applied. However, the duration of an infiltrometer experiment is often established quite subjectively based on the assumption that in general infiltration stabilizes rather quickly in the field. For a loam soil, the effect of the duration of a beerkan run on sorptivity, S, and saturated hydraulic conductivity, Ks, was tested by using the BEST (Beerkan Estimation of Soil Transfer parameters)-steady method and SSBI (Steady version of the Simplified method based on a Beerkan Infiltration run) method for data analysis. The standard experiment, based on a total of 15 water volumes each establishing an initial pon…