6533b7dcfe1ef96bd12731e7
RESEARCH PRODUCT
Evaluation of the use of phase-specific gene promoters for the expression of enological enzymes in an industrial wine yeast strain
Amparo QuerolSergi PuigJosé E. Pérez-ortínJosé E. Pérez-ortínDaniel Ramónsubject
Reporter geneSaccharomyces cerevisiaeBioengineeringPromoterGeneral MedicineBiologybiology.organism_classificationApplied Microbiology and BiotechnologyEnzyme assayYeast in winemakingBiochemistryGene expressionbiology.proteinFermentationGeneBiotechnologydescription
Genes as POT1, HSP104 and SSA3, which are late expressed in laboratory culture conditions are expressed only during the first few days in microvinifications in wine yeast cells. This effect is probably due to the different growth conditions and leads to useless levels of enzyme activity for a reporter gene. However the ACT1 promoter, which is constitutively expressed in laboratory conditions, produces sufficient amounts of enzyme activity in late fermentation phases.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1996-08-01 | Biotechnology Letters |