Search results for "Bioengineering"
showing 10 items of 1963 documents
Identification ofCandida albicanswall mannoproteins covalently linked by disulphide and/or alkali-sensitive bridges
2014
This paper describes the results obtained by analysing the human pathogen Candida albicans cell wall subproteome by mass spectrometry, using extraction procedures aimed at releasing proteins bound by disulphide bridges (RAE-CWP) or alkali-labile ester linkages (ALS-CWP). Ten of the total proteins released from the wall by β-ME and/or NaOH contained a potential signal peptide, lacked a GPI cell wall hydrophobic C-terminal domain and were identified as true wall proteins by in silico analysis, whereas four additional proteins were identified as bound to the plasma membrane. The results surprisingly demonstrated that, in addition to the expected RAE-CWP and ALS-CWP proteins, 16 GPI proteins we…
Information Processing Schemes Based on Monolayer Protected Metallic Nanoclusters
2011
Nanostructures are potentially useful as building blocks to complement future electronics because of their high versatility and packing densities. The fabrication and characterization of particular nanostructures and the use of new theoretical tools to describe their properties are receiving much attention. However, the integration of these individual systems into general schemes that could perform simple tasks is also necessary because modern electronics operation relies on the concerted action of many basic units. We review here new conceptual schemes that can allow information processing with ligand or monolayer protected metallic nanoclusters (MPCs) on the basis of the experimentally de…
Effect of micro-aeration and leachate replacement on COD solubilization and VFA production during mono-digestion of grass-silage in one-stage leach-b…
2010
The effect of micro-aeration and leachate replacement with fresh water on chemical oxygen demand (COD) solubilization and volatile fatty acid (VFA) production during the mono-digestion of grass-silage in one-stage leach-bed reactors (LBRs) was investigated in four LBRs, L0 (control), L1, L2 and L3 in batch mode at 35 + or - 1 degrees Celsius for 57 days. Results showed that leachate replacement without pH adjustment (L3) resulted in 2.7 and 1.3 times more SCOD in the leachate compared to control (L0) or leachate replacement with initial pH adjustment (L1), respectively. Micro-aeration at flow rate of 1 L min(-1) (2.5 L of air) in L2 resulted in 4-fold increase in VFA production (from 2.2 to…
Optical tweezing using tunable optical lattices along a few-mode silicon waveguide
2018
International audience; Fourteen years ago, optical lattices and holographic tweezers were considered as a revolution, allowing for trapping andmanipulating multiple particles at the same time using laser light. Since then, near-field optical forces have arousedtremendous interest as they enable efficient trapping of a wide range of objects, from living cells to atoms, in integrateddevices. Yet, handling at will multiple objects using a guided light beam remains a challenging task for current on-chipoptical trapping techniques. We demonstrate here on-chip optical trapping of dielectric microbeads and bacteria usingone-dimensional optical lattices created by near-field mode beating along a f…
Neural cell pattern formation on glass and oxidized silicon surfaces modified with poly(N-isopropylacrylamide)
1996
Control over the adsorption of proteins and over the adsorption and spatial orientation of mammalian cells onto surfaces has been achieved by modification of glass and other silicon oxide substrates with poly(N-isopropylacrylamide) (PNIPAM). The functionalization of the substrates was achieved either by a polymer-analogous reaction of aminosilanes with reactive N-(isopropylacrylamide) (NIPAM)-copolymers and by copolymerization of NIPAM with surface-bound methacrylsilane. The obtained coatings were characterized by FT-1R, ellipsometry, and surface plasmon resonance measurements. The adsorption of two proteins-fibrinogen and ribonuclease A-on these surfaces was studied in situ by real time su…
Ultrasensitive Silicon Nanowire for Real-World Gas Sensing: Noninvasive Diagnosis of Cancer from Breath Volatolome
2014
We report on an ultrasensitive, molecularly modified silicon nanowire field effect transistor that brings together the lock-and-key and cross-reactive sensing worlds for the diagnosis of (gastric) cancer from exhaled volatolome. The sensor is able to selectively detect volatile organic compounds (VOCs) that are linked with gastric cancer conditions in exhaled breath and to discriminate them from environmental VOCs that exist in exhaled breath samples but do not relate to the gastric cancer per se. Using breath samples collected from actual patients with gastric cancer and from volunteers who do not have cancer, blind analysis validated the ability of the reported sensor to discriminate betw…
Green synthesis of silver nanoparticles mediated by Pulicaria glutinosa extract
2013
Mujeeb Khan,1 Merajuddin Khan,1 Syed Farooq Adil,1 Muhammad Nawaz Tahir,2 Wolfgang Tremel,2 Hamad Z Alkhathlan,1 Abdulrahman Al-Warthan,1 Mohammed Rafiq H Siddiqui1 1Department of Chemistry, College of Science, King Saud University, Riyadh, Kingdom of Saudi Arabia; 2Institute of Inorganic and Analytical Chemistry, Johannes Gutenberg-University of Mainz, Mainz, Germany Abstract: The green synthesis of metallic nanoparticles (NPs) has attracted tremendous attention in recent years because these protocols are low cost and more environmentally friendly than standard methods of synthesis. In this article, we report a simple and eco-friendly method for the synthesis of silver NPs using an aqueous…
Separation of Nanoparticles by Gel Electrophoresis According to Size and Shape
2007
We demonstrate the separation of gold and silver nanoparticles according to their size and shape by agarose gel electrophoresis after coating them with a charged polymer layer. The separation is monitored optically using the size- and shape-dependent plasmon resonance of noble metal particles and confirmed by transmission electron microscopy (TEM). Electrophoretic mobilities are quantitatively explained by a model based on the Henry formula, providing a theoretical framework for predicting gel mobilities of polymer coated nanoparticles.
Plasmonic Focusing Reduces Ensemble Linewidth of Silver-Coated Gold Nanorods
2008
Silver coating gold nanorods reduces the ensemble plasmon line width by changing the relation connecting particle shape and plasmon resonance wavelength. This change, we term "plasmonic focusing", leads to less variation of resonance wavelengths for the same particle size distribution. We also find smaller single particle linewidth comparing resonances at the same wavelength but show that this does not contribute to the ensemble linewidth narrowing.
Nylon-Supported Plasmonic Assay Based on the Aggregation of Silver Nanoparticles: In Situ Determination of Hydrogen Sulfide-like Compounds in Breath …
2019
A procedure for supporting silver nanoparticles (AgNPs) on nylon is proposed. Besides, the membrane has been developed as a solid-phase colorimetric plasmonic sensor for volatile sulfide compounds (VSCs) like H2S, CH3SH, and (CH3)2S. AgNP behavior in the membrane has been studied by UV-vis diffuse reflectance spectrometry, Raman spectrometry, High-resolution transmission electron microscopy (HR-TEM), and Scanning electron microscopy (SEM). The sensor responded by changing its color from yellow in absence of VSCs to several orange/brown colors in the function of VSC concentration as occurs in solution; an increase in the hydrodynamic diameter, estimated by both asymmetrical flow field-flow f…