Search results for "Biomedical"
showing 10 items of 2328 documents
Engineering in-plane mechanics of electrospun polyurethane scaffolds for cardiovascular tissue applications
2021
Effective cardiovascular tissue surrogates require high control of scaffold structural and mechanical features to match native tissue properties, which are dependent on tissue-specific mechanics, function heterogenicity, and morphology. Bridging scaffold processing variables with native tissue properties is recognized as a priority for advancing biomechanical performance of biomedical materials and, when translated to the clinical practice, their efficacy. Accordingly, this study selected electrospinning on a rotating cylindrical target as an apparatus of broad application and mapped the relationship between key processing variables and scaffold mechanics and structure. This information was…
Dorsal root ganglia neurite outgrowth measured as a function of changes in microelectrode array resistance
2017
Current research in prosthetic device design aims to mimic natural movements using a feedback system that connects to the patient's own nerves to control the device. The first step in using neurons to control motion is to make and maintain contact between neurons and the feedback sensors. Therefore, the goal of this project was to determine if changes in electrode resistance could be detected when a neuron extended a neurite to contact a sensor. Dorsal root ganglia (DRG) were harvested from chick embryos and cultured on a collagen-coated carbon nanotube microelectrode array for two days. The DRG were seeded along one side of the array so the processes extended across the array, contacting a…
Obstáculos à produtividade científica de professoras universitárias da área de Ciências da Saúde
2013
To discover the features associated with personal and professional lives of university teachers, in the area of health sciences in the “Comunidad Valenciana” for the period 2003-2007, to learn if they contribute to a greater or lesser scientific productivity. Were interviewed in depth 30 teachers aged between 30 and 60 years in order to identify factors that hamper productivity. Have more difficulties to publish scientific papers, teachers less productive. Represent obstacles: the labor problems, the difficulty to obtain science projects and to publish in certain journals, to balance teaching and research, care work in hospitals, the maternity and children. Although the two groups of large …
Isolation of fibroblasts for coating of meshes for reconstructive surgery: differences between mesh types.
2009
Aims: An extensive colonization of surgical meshes with autologous fibroblasts may reduce complications. Therefore, we aimed to establish a technique that allows isolation and propagation of fibroblasts from vaginal biopsies. Using these cells we tested the applicability of several clinically applied meshes for fibroblast coating. Materials & methods: Fibroblasts were isolated from vaginal tissue after digestion with collagenase. Characterization was performed by immunostaining for cytokeratin 5, 6 and 14, smooth muscle actin and vimentin. A semiquantitative technique was applied to determine the degree of mesh coating 5 h and 5 weeks after seeding of fibroblasts. Seven meshes of diffe…
Crossing frontiers in biomaterials and regenerative medicine
2008
From 28 May–1 June 2008, the 8th World Biomaterials Congress (WBC) was held in Amsterdam, The Netherlands. The meeting attracted 3052 participants from 82 countries, which has made this congress the largest meeting ever held in the field of biomaterials and regenerative medicine. More than 650 oral and 1500 poster contributions [1] were presented in 108 scientific sessions, divided into nine parallel sessions, including 48 dedicated symposia that had been proposed by international leaders in the field. The symposia each contained an invited keynote, providing expert opinions on current developments in biomaterials research. Historically, research in the field of biomaterials has focused on …
Synthetic microbiology as a source of new enterprises and job creation: a Mediterranean perspective
2019
Peer reviewed
Dendritic cells, engineered to secrete a T-cell receptor mimic peptide, induce antigen-specific immunosuppression in vivo
2003
A T-cell receptor mimic peptide (TCRpep) consisting of an 8-amino-acid peptide, homologous to the transmembrane region of the T-cell receptor (TCR) alpha chain, blocks T-cell activation after systemic application. When dendritic cells (DCs) were transduced to secrete the TCRpep and injected into mice, evidence of immunosuppression was observed. In a CD8-driven allergy model, the injection of DCs transduced with the TCRpep reduced inflammation markedly and in a CD4+ T cell-dependent model of multiple sclerosis (experimental autoimmune encephalitis, EAE), injection of TCRpep-secreting DCs abrogated EAE symptoms and prolonged survival. These effects were antigen specific, because transduced DC…
Enrichment of Outgrowth Endothelial Cells in High and Low Colony-Forming Cultures from Peripheral Blood Progenitors
2009
An effective isolation protocol for outgrowth endothelial cells (OEC) resulting in higher cell numbers and a reduced expansion time would facilitate the therapeutical application. In this study a standard protocol based on the isolation of mononuclear cells from adult peripheral blood was modified by adding a passaging step 7 days after the isolation. OEC colonies gained by both protocols were evaluated after 28 days and resulted in different frequencies of OEC colonies depending on the donor and culture protocol. Accordingly, we defined two groups, namely, high colony-forming cultures (HCC) and low colony-forming cultures (LCC) for further analysis. LCC revealed no increase in OEC colonies…
VALIDITY, RELIABILITY AND FEASIBILITY OF MEASURING MUSCLE ACTIVITY WITH TEXTILE ELECTRODES EMBEDDED INTO CLOTHING
2007
INTRODUCTION In out-of-laboratory settings the surface EMG measurements are complicated by the requirement of careful electrode placement and skin preparation. The development of washable textile electrodes that can be sowed into sportswear enable muscle activity recordings during normal locomotion without skin preparation and the problem of wires hanging around the body [1]. In this study we performed validity, reliability and feasibility tests of the textile electrodes embedded into shorts against the traditional bipolar surface electrodes.
Increasing understanding and confidence in THM simulations of engineered barrier systems
2020
Previous studies on the modelling of coupled thermo-hydro-mechanical (THM) processes in bentonite-based engineered barrier systems (EBSs) showed the sensitivity of the output quantities to changes in the input parameters. To investigate the effects of uncertainties on the modelling results, to improve the understanding of the coupled processes active in the repository near field and to gain in-depth understanding of model uncertainties of different codes, a sensitivity analysis and code comparison of EBS simulations was performed within the Task Force on Engineered Barrier Systems. The analysis included variations in material parameter values, boundary and initial conditions, considered phy…