Search results for "Bios"

Stress response in Drosophila subobscura

1988

The pattern of puffing and protein synthesis was determined in individuals of Drosophila subobscura subjected to heat shock. Depending on the extent of the heat treatment, the response at the puffing level varied. Some puffs were expressed at 31°–34°C, and others at 37° C. Considering the response as a whole the depression of gene activity after shock at 31°–34° C in individuals raised at 19° C was greater than with the other treatments. Six major heat shock proteins (Hsps) were found in this species. The properties of the high molecular weight proteins are conserved their electrophoretic characteristics and the range of temperatures over which they are synthesized are close to those in oth…

Chronology for the Cueva Victoria fossil site (SE Spain): Evidence for Early Pleistocene Afro-Iberian dispersals

2014

Cueva Victoria has provided remains of more than 90 species of fossil vertebrates, including a hominin phalanx, and the only specimens of the African cercopithecid Theropithecus oswaldi in Europe. To constrain the age of the vertebrate remains we used paleomagnetism, vertebrate biostratigraphy and (230)Th/U dating. Normal polarity was identified in the non-fossiliferous lowest and highest stratigraphic units (red clay and capping flowstones) while reverse polarity was found in the intermediate stratigraphic unit (fossiliferous breccia). A lower polarity change occurred during the deposition of the decalcification clay, when the cave was closed and karstification was active. A second polarit…

Nano-structured molecular thin films for bio-sensing and plastic-electronics with improved efficiency/cost ratio

2008

Combinatorial Biosynthesis of Polyketides

2003

NG2 regulates directional migration of oligodendrocyte precursor cells via Rho GTPases and polarity complex proteins.

2013

The transmembrane proteoglycan NG2 is expressed by oligodendrocyte precursor cells (OPC), which migrate to axons during developmental myelination and remyelinate in the adult after migration to injured sites. Highly invasive glial tumors also express NG2. Despite the fact that NG2 has been implicated in control of OPC migration, its mode of action remains unknown. Here, we show in vitro and in vivo that NG2 controls migration of OPC through the regulation of cell polarity. In stab wounds in adult mice we show that NG2 controls orientation of OPC toward the wound. NG2 stimulates RhoA activity at the cell periphery via the MUPP1/Syx1 signaling pathway, which favors the bipolar shape of migrat…

A two miRNA classifier differentiates follicular thyroid carcinomas from follicular thyroid adenomas.

2015

The inherent diagnostic limitations of thyroid fine needle aspiration (FNA), especially in the "indeterminate" category, can be partially overcome by molecular analyses. We aimed at the identification of miRNAs that could be used to improve the discrimination of indeterminate FNAs. miRNA expression profiling was performed for 17 follicular carcinomas (FTCs) and 8 follicular adenomas (FAs). The microarray results underwent cross-comparison using three additional microarray data sets. Candidate miRNAs were validated by qPCR in an independent set of 32 FTCs and 46 FAs. Sixty-eight differentially expressed miRNAs were identified. Thirteen miRNAs could be confirmed by cross comparison. A two-miR…

Toward development of optical biosensors based on photoluminescence of TiO2 nanoparticles for the detection of Salmonella

2017

Quality control of food and agriculture production is an inseparable part of human safety and wellbeing. Salmonella infections belong to one of the most monitored pathogens in the world, therefore advanced determination of this pathogen can decrease the risks of human diseases caused by this microorganism. In this research we introduce a novel optical immunosensor for determination of Salmonella typhimurium. The immunosensor is based on Titanium dioxide (TiO2) nanoparticles deposited on glass substrates (glass/TiO2)center dot TiO2 nanoparticles exhibit an intense photoluminescence (PL) in the visible range of spectrum at room temperature. The direct immobilization of antibodies (anti-S-Ab) …

Changes of the hindgut microbiota due to high-starch diet can be associated with behavioral stress response in horses.

2015

6 pages; International audience; The digestive system of horses is adapted to a high-fiber diet consumed in small amounts over a long time. However, during training, high-starch and low-fiber diets are usually fed which may induce hindgut microbial disturbances and intestinal pain. These diets can be described as alimentary stress. The aim of the present study was to investigate to what extent changes in behavior are associated with alimentary stress and microbial composition changes of the cecal or colonic ecosystem. Six fistulated horses were used. The alimentary stress was a modification of diet from a high-fiber diet (100% hay) to a progressive low-fiber and high-starch diet (from 90% h…

Species-specific aggregation factor in sponges V. Influence on programmed syntheses

1976

Isolated cells from the siliceous sponge Geodia cydonium as well as small primary aggregates (diameter: 70 mum) consisting of them show no increase in rates of programmed syntheses and mitotic activity with time. After addition of a highly purified aggregation factor to a culture with primary aggregates which subsequently form secondary aggregates (diameter: larger than 1000 mum), a dramatic increase of DNA, RNA and protein synthesis occurs. Together with this increase, the cells show a high mitotic activity. The values for the mitotic coefficient reach a first maximum 8 h after the beginning of the secondary aggregation process. The stimulation of the mitotic activity of cells during the a…

Inhibition of giant cell formation by compound 48/80 after infection with herpesvirus hominis

1974

Choline kinase has been found to be a soluble enzyme with a molecular weight of 105,000 in the cytoplasm of primary rabbit kidney cells. It has been purified 150-fold. It was investigated whether the inhibiting effect of Cpd 48/80 on virus-induced giant cell formation is due to interference with this enzyme. Cpd 48/80-dimer was shown to inhibit the choline kinase activityin vitro without a concomitant inhibition of giant cell formation. Likewise, another competitive inhibitor of choline kinase, purinyl-6-histamine, does not prevent giant cell formation. This finding suggests that there is no correlation between choline kinase activity and giant cell formation.