6533b860fe1ef96bd12c392b
RESEARCH PRODUCT
Species-specific aggregation factor in sponges V. Influence on programmed syntheses
Werner E. G. M�llerIsabel M. MüllerRudolf K. Zahnsubject
Time FactorsCellPopulationStimulationBiologyModels BiologicalBiochemistry Genetics and Molecular Biology (miscellaneous)Bleomycinchemistry.chemical_compoundSpecies SpecificitymedicineProtein biosynthesisAnimalseducationMitosisCell Aggregationeducation.field_of_studyDNA synthesisRNADNAPoriferamedicine.anatomical_structurechemistryBiochemistryProtein BiosynthesisDactinomycinBiophysicsRNAPuromycinColchicineCell DivisionDNAdescription
Isolated cells from the siliceous sponge Geodia cydonium as well as small primary aggregates (diameter: 70 mum) consisting of them show no increase in rates of programmed syntheses and mitotic activity with time. After addition of a highly purified aggregation factor to a culture with primary aggregates which subsequently form secondary aggregates (diameter: larger than 1000 mum), a dramatic increase of DNA, RNA and protein synthesis occurs. Together with this increase, the cells show a high mitotic activity. The values for the mitotic coefficient reach a first maximum 8 h after the beginning of the secondary aggregation process. The stimulation of the mitotic activity of cells during the aggregation factor induced secondary aggregation process can be suppressed by inhibitors of RNA and protein synthesis as well as by a blocker of DNA synthesis. This finding may indicate that cells from the G0-population enter the proliferating cell pool via the G1-phase.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 1976-01-19 | Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis |