Search results for "Bios"
showing 10 items of 2557 documents
Low levels of both xanthine dehydrogenase and of cellular retinol binding protein are responsible for retinoic acid deficiency in malignant human mam…
2009
The seeming impairment of retinoid metabolism in human breast tumor cells has been attributed to the lower expression of cellular retinol binding proteins (CRBPs), of alcohol/retinol dehydrogenases, or aldehyde/retinaldehyde dehydrogenases. In a previous study we indicated that xanthine dehydrogenase (XDH) is able to oxidize actively both all-trans-retinol (t-ROL) bound to the CRBP (holo-CRBP) and all-trans-retinaldehyde (t-RAL) to all-trans-retinoic acid (t-RA) in human mammary epithelial cells (HMEC). Since both XDH and CRBP are required for the biosynthesis of t-RA, we have inspected their bioavailability in both estrogen-responsive and nonresponsive human mammary epithelial cancer cells…
Photoluminescent Detection of Human T-Lymphoblastic Cells by ZnO Nanorods.
2020
The precise detection of cancer cells currently remains a global challenge. One-dimensional (1D) semiconductor nanostructures (e.g., ZnO nanorods) have attracted attention due to their potential use in cancer biosensors. In the current study, it was demonstrated that the possibility of a photoluminescent detection of human leukemic T-cells by using a zinc oxide nanorods (ZnO NRs) platform. Monoclonal antibodies (MABs) anti-CD5 against a cluster of differentiation (CD) proteins on the pathologic cell surface have been used as a bioselective layer on the ZnO surface. The optimal concentration of the protein anti-CD5 to form an effective bioselective layer on the ZnO NRs surface was selected. …
Desarrollo de Aptazovid: aptasensor piezoeléctrico para la detección de SARS-CoV-2
2021
SARS-CoV-2 ha dado lugar a una pandemia en la que la detección y aislamiento de personas infectadas es clave para luchar contra el virus. La detección puede ser directa o indirecta con diferentes dianas y muestras de partida, aplicando técnicas tan conocidas como la RT-PCR o desarrollando nuevos métodos que integran herramientas como CRISPR, aunque con ciertas limitaciones. Desde hace algún tiempo, los biosensores están en auge y la pandemia ha favorecido la inversión en el desarrollo de tecnologías que superen los inconvenientes de metodologías más tradicionales. En este contexto, como solución surge Aptazovid, un aptasensor piezoeléctrico que detecta directamente la glicoproteína de super…
Survival, Growth and Mycorrhization of Containerised Pinus sylvestris and Picea abies Seedlings of Different Provenances Outplanted in a Forest Clear…
2017
Abstract We studied field performance of containerised Pinus sylvestris and Picea abies seedlings of different provenances. Shoot height, needle chemical composition, root collar diameter, root mycorrhization and mycorrhizal species composition were evaluated after four growing seasons following outplanting. The results showed that in general spruce had better survival than pine. Ectomycorrhizas on spruce were dominated by Wilcoxina, Amphinema and Tylospora, while on pine — by Suillus and Thelephora species. Spruce and pine showing best growth rates were colonised by ectomycorrhizal fungus Amphinema sp. In conclusion, the results demonstrated that forest nursery practices as well as provena…
Nuevas faunas de micromamíferos del Terciario continental de la Depresión Intermedia (provincia de Cuenca, España centro-oriental)
1986
In this paper the fossil content of nine new micromammal bearing localities and that of 15 levels in a borehole from the Loranca Basin (eastern Tague Basin, Prov. of Cuenca) is described. The age ranges from early Arvernian (Late Oligocene) to Turolian (Late Miocene). 47 rodent and lagomorph taxons have been found which are arranged in 8 biostratigraphic units. The Loranca-2 borehole contains 15 fossiliferous levels covering the Upper Oligocene/Lower Miocene. These additional data complete the faunal sequence published by Díaz & López(1919). The stratigraphic framework of the basin needs a revision because of the new faunal data.<br><br>Se presenta una serie de 9 nuevos yaci…
Influence of a Thiolate Chemical Layer on GaAs (100) Biofunctionalization: An Original Approach Coupling Atomic Force Microscopy and Mass Spectrometr…
2013
International audience; Widely used in microelectronics and optoelectronics; Gallium Arsenide (GaAs) is a III-V crystal with several interesting properties for microsystem and biosensor applications. Among these; its piezoelectric properties and the ability to directly biofunctionalize the bare surface, offer an opportunity to combine a highly sensitive transducer with a specific bio-interface; which are the two essential parts of a biosensor. To optimize the biorecognition part; it is necessary to control protein coverage and the binding affinity of the protein layer on the GaAs surface. In this paper; we investigate the potential of a specific chemical interface composed of thiolate molec…
Study of the role of phosphorylated pathway of serine biosynthesis in arabidopsis development and its connections with nitrogen and carbon metabolism
2016
En plantas se han descrito tres rutas diferentes de para la síntesis de serina: la ruta del glicolato, asociada a la fotorrespiración, y dos rutas independientes de ella, la ruta del glicerato y la ruta fosforilativa de biosíntesis de serina (PPSB). Como la ruta del glicolato es la más importante en términos cuantitativos, el papel de la PPSB ha sido ignorado hasta hace unos años. Esta tesis doctoral aporta nuevas perspectivas sobre la función de la PPSB tanto en el desarrollo como en el metabolismo de Arabidopsis. La PPSB se abastece de 3-fosfoglicerato (3-PGA) como sustrato, el cual puede ser suministrado por la glicolisis plastidial o citosólica. Se asume que la actividad de estas dos ru…
Caratterizzazione del contenuto aromatico e dei profili trascrizionali di geni coinvolti nella biosintesi degli aromi durante lo sviluppo dell’acino …
2010
Behaviour of sediment-associated silver nanoparticles and their toxicity to Lumbriculus variegatus
2017
The increasing use of silver nanoparticles (AgNPs) in industry and consumer products results in the increasing environmental concentrations of AgNPs. In the aquatic environment, sediment is the major sink for the AgNPs. The behaviour and toxicity of AgNPs in sediments is largely unknown, and therefore the potential risk to the benthic ecosystems is difficult to predict. The aim of this thesis was to study the toxicity and uptake of uncoated AgNPs (uc-AgNPs) and polyvinylpyrrolidone-coated AgNPs (PVP-AgNPs) to Lumbriculus variegatus in sediment exposures. Also, the association of AgNP-originated dissolved Ag with sediment components was studied using a sequential extraction method. Studies w…